CDK15 (NM_139158) Human Untagged Clone

CAT#: SC323695

CDK15 (untagged)-Kinase deficient mutant (K81M) of Human PFTAIRE protein kinase 2 (PFTK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK15
• Synonyms: ALS2CR7; PFTAIRE2; PFTK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323695 sequence for NM_139158 edited (data generated by NextGen Sequencing)
  ATGACTTCATTTCACCCCAGGGGACTTCAAGCTGCCCGTGCCCAGAAGTTCAAGAGTAAA
  AGGCCACGGAGTAACAGTGATTGTTTTCAGGAAGAGGATCTGAGGCAGGGTTTTCAGTGG
  AGGAAGAGCCTCCCTTTTGGGGCAGCCTCATCTTACTTGAACTTGGAGAAGCTGGGTGAA
  GGCTCTTATGCGACAGTTTACAAGGGGATTAGCAGAATAAATGGACAACTAGTGGCTTTA
  AWRGTCATCAGCATGAATGCAGAGGAAGGAGTCCCATTTACAGCTATCCGAGAAGCTTCT
  CTCCTGAAGGGTTTGAAACATGCCAATATTGTGCTCCTGCATGACATAATCCACACCAAA
  GAGACACTGACATTCGTTTTTGAATACATGCACACAGACCTGGCCCAGTATATGTCTCAG
  CATCCAGGAGGGCTTCATCCTCATAATGTCAGACTTTTCATGTTTCAACTTTTGCGGGGC
  CTGGCGTACATCCACCACCAACACGTTCTTCACAGGGACCTGAAACCTCAGAACTTACTC
  ATCAGTCACCTGGGAGAGCTCAAACTGGCTGATTTTGGTCTTGCCCGGGCCAAGTCCATT
  CCCAGCCAGACATACTCTTCAGAAGTCGTGACCCTCTGGTACCGGCCCCCTGATGCTTTG
  CTGGGAGCCACTGAATATTCCTCTGAGCTGGACATATGGGGTGCAGGCTGCATCTTTATT
  GAAATGTTCCAGGGTCAACCTTTGTTTCCTGGGGTTTCCAACATCCTTGAACAGCTGGAG
  AAAATCTGGGAGGTGCTGGGAGTCCCTACAGAGGATACTTGGCCGGGAGTCTCCAAGCTA
  CCTAACTACAATCCAGAATGGTTCCCACTGCCTACGCCTCGAAGCCTTCATGTTGTCTGG
  AACAGGCTGGGCAGGGTTCCTGAAGCTGAAGACCTGGCCTCCCAGATGCTAAAAGGCTTT
  CCCAGAGACCGCGTCTCCGCCCAGGAAGCACTTGTTCATGATTATTTCAGCGCCCTGCCA
  TCTCAGCTGTACCAGCTTCCTGATGAGGAGTCTTTGTTTACAGTTTCAGGAGTGAGGCTA
  AAGCCAGAAATGTGTGACCTTTTGGCCTCCTACCAGAAAGGTCACCACCCAGCCCAGTTT
  AGCAAATGCTGGTGA
  
  Clone variation with respect to NM_139158.1
  242 a=>w;243 a=>r
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_139158 unedited
  ACCGCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAGGAGTCCCCAGAG
  AGGCAGGAGGAAAACCAAGGAAGTGCCCACTTCACAGAATCAAGGAGGCTTTCAAAAAGGGTGGCATGAT
  TATGGTGTTAAATGCCACCAAAGAGTAAAGCAAGGACTCTTGCTCTGTCACCCAGTCTGAAGTGCTGTGG
  CGCAACCATGGCTCTATGCAGCCTTGACCTCCTGAACTCAAGCAATTGTCCCTGCCTCAGCCTTCCCAGT
  TGCTGGGACCGCGGCTGCCTCGTTTTCAGTTTGTGCACATCTGATTGCCAAGCAATCCCGGTCTGAGGTG
  GAAGTTTCTTGCACTGATAAGGAAAAACTGCTGAGGTTGTGAGGCTGCTCCAAGGCCGAAGCATCATCCT
  ACCGGACTAAAAAGAAGGCTCATGGTTCCAGAGACTTCATTTACCCAAGGGAATCTTAAGCCGCGCCCTG
  TGCCAGAAATTTCAGAGAGTAAAAGGCACAGGGATATAACGTGTGATGTGTTTTCGAGAAGAGTCTCGAG
  GCACAGGTTTCAGTGGGGCGGAAGGGGAGACCCTCCTTTGGGCGCGCCCTCATCTCTTTAGATTTGGAAG
  ACTGTTGAAGCGCTCTTGCGCAGTTTCACAGGGGTATCACAGAATAGGCACTCTGTGTCTATAGTGTCTC
  ACGATGCCAGAGAGTCTCTATACTTCCAAGCTTCTCTCGAGGTTGAATGCACATGGTGCTGCGTGGATAT
  ACCACAGACCTCGATCTCGGTTGATCGACGAACTGCAAATTCATCAGGCTTCTATGGCACTAGTGACATC
  GGCTGCATCCACAGCGTAAGAACGAACTGACATCCCTA
  
  Kinase domain sequence

  >SC323695 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CTCGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAGGAGTCCCCAGAGAGGCAGG
  AGGAAAACCAAGGAAGTGCCCACTTCACAGAATCAAGGAGGCTTTCAAAAAGGGTGGCATGATTATGGTG
  TTAAATGCCACCAAAGAGTAAAGCAAGGATCTTGCTCTGTCACCC
  
• Restriction Sites: Please inquire
• ACCN: NM_139158
• ORF Size: 1155 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_139158.1, NP_631897.1
• RefSeq Size: 1534
• RefSeq ORF: 1155
• Locus ID: 65061
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Serine/threonine-protein kinase that acts like an antiapoptotic protein that counters TRAIL/TNFSF10-induced apoptosis by inducing phosphorylation of BIRC5 at 'Thr-34'. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has an alternate in-frame 5' exon and initiates translation at an upstream AUG compared to variant 1. This results in a shorter protein (isoform 3) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.