MTHFD2 (NM_006636) Human Untagged Clone

CAT#: SC323742

MTHFD2 (untagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTHFD2
• Synonyms: NMDMC
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006636 edited
  CGGTCTATGGCTGCGACTTCTCTAATGTCTGCTTTGGCTGCCCGGCTGCTGCAGCCCGCG
  CACAGCTGCTCCCTTCGCCTTCGCCCTTTCCACCTCGCGGCAGTTCGAAATGAAGCTGTT
  GTCATTTCTGGAAGGAAACTGGCCCAGCAGATCAAGCAGGAAGTGCGGCAGGAGGTAGAA
  GAGTGGGTGGCCTCAGGCAACAAACGGCCACACCTGAGTGTGATCCTGGTTGGCGAGAAT
  CCTGCAAGTCACTCCTATGTCCTCAACAAAACCAGGGCAGCTGCAGTTGTGGGAATCAAC
  AGTGAGACAATTATGAAACCAGCTTCAATTTCAGAGGAAGAATTGTTGAATTTAATCAAT
  AAACTGAATAATGATGATAATGTAGATGGCCTCCTTGTTCAGTTGCCTCTTCCAGAGCAT
  ATTGATGAGAGAAGGATCTGCAATGCTGTTTCTCCAGACAAGGATGTTGATGGCTTTCAT
  GTAATTAATGTAGGACGAATGTGTTTGGATCAGTATTCCATGTTACCGGCTACTCCATGG
  GGTGTGTGGGAAATAATCAAGCGAACTGGCATTCCAACCCTAGGGAAGAATGTGGTTGTG
  GCTGGAAGGTCAAAAAACGTTGGAATGCCCATTGCAATGTTACTGCACACAGATGGGGCG
  CATGAACGTCCCGGAGGTGATGCCACTGTTACAATATCTCATCGATATACTCCCAAAGAG
  CAGTTGAAGAAACATACAATTCTTGCAGATATTGTAATATCTGCTGCAGGTATTCCAAAT
  CTGATCACAGCAGATATGATCAAGGAAGGAGCAGCAGTCATTGATGTGGGAATAAATAGA
  GTTCACGATCCTGTAACTGCCAAACCCAAGTTGGTTGGAGATGTGGATTTTGAAGGAGTC
  AGACAAAAAGCTGGGTATATCACTCCAGTTCCTGGAGGTGTTGGCCCCATGACAGTGGCA
  ATGCTAATGAAGAATACCATTATTGCTGCAAAAAAGGTGCTGAGGCTTGAAGAGCGAGAA
  GTGCTGAAGTCTAAAGAGCTTGGGGTAGCCACTAATTAACTACTGTGTCTTCTGTGTCAC
  AAACAGCACTCCAGGCCAGCTCAAGAAGCAAAGCAGGCCAATAGAAATGCAATATTTTTA
  ATTTATTCTACTGAAATGGTTTAAAATGATGCCTTGTATTTATTGAAAGCTTAAATGGGT
  GGGTGTTTCTGCACATACCTCTGCAGTACCTCACCAGGGAGCATTCCAGTATCATGCAGG
  GTCCTGTGATCTAGCCAGGAGCAGCCATTAACCTAGTGATTAATATGGGAGACATTACCA
  TATGGAGGATGGATGCTTCACTTTGTCAAGCACCTCAGTTACACATTCGCCTTTTCTAGG
  ATTGCATTTCCCAAGTGCTATTGCAATAACAGTTGATACTCATTTTAGGTACCAAACCTT
  TTGAGTTCAACTGATCAAACCAAAGGAAAAGTGTTGCTAGAGAAAATTAGGGAAAAGGTG
  AAAAAGAAAAAATGGTAGTAATTGAGCAGAAAAAAATTAATTTATATATGTATTGATTGG
  CAACCAGATTTATCTAAGTAGAACTGAATTGGCTAGGAAAAAAGAAAAACTGCATGTTAA
  TCATTTTCCTAAGCTGTCCTTTTGAGGCTTAGTCAGTTTATTGGGAAAATGTTTAGGATT
  ATTCCTTGCTATTAGTACTCATTTTATGTATGTTACCCTTCAGTAAGTTCTCCCCATTTT
  AGTTTTCTAGGACTGAAAGGATTCTTTTCTACATTATACATGTGTGTTGTCATATTTGGC
  TTTTGCTATATACTTTAACTTCATTGTTAAATTTTTGTATTGTATAGTTTCTTTGGTGTA
  TCTTAAAACCTATTTTTGAAAAACAAACTTGGCTTGATAATCATTTGGGCAGCTTGGGTA
  AGTACGCAACTTACTTTTCCACCAAAGAACTGTCAGCAGCTGCCTGCTTTTCTGTGATGT
  ATGTATCCTGTTGACTTTTCCAGAAATTTTTTAAGAGTTTGAGTTACTATTGAATTTAAT
  CAGACTTTCTGATTAAAGGGTTTTCTTTCTTTTTTAATAAAACACATCTGTCTGGTATGG
  TAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006636.3 unedited
  GACATTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGTCCGG
  AATTCCCGGGATATCGTCGACCCACGCGTCCGGCGGTCTATGGCTGCGACTTCTCTAATG
  TCTGCTTTGGCTGCCCGGCTGCTGCAGCCCGCGCACAGCTGCTCCCTTCGCCTTCGCCCT
  TTCCACCTCGCGGCAGTTCGAAATGAAGCTGTTGTCATTTCTGGAAGGAAACTGGCCCAG
  CAGATCAAGCAGGAAGTGCGGCAGGAGGTAGAAGAGTGGGTGGCCTCAGGCAACAAACGG
  CCACACCTGAGTGTGATCCTGGTTGGCGAGAATCCTGCAAGTCACTCCTATGTCCTCAAC
  AAAACCAGGGCAGCTGCAGTTGTGGGAATCAACAGTGAGACAATTATGAAACCAGCTTCA
  ATTTCAGAGGAAGAATTGTTGAATTTAATCAATAAACTGAATAATGATGATAATGTAGAT
  GGCCTCCTTGTTCAGTTGCCTCTTCCAGAGCATATTGATGAGAGAAGGATCTGCAATGCT
  GTTTCTCCAGACAAGGATGTTGATGGCTTTCATGTAATTAATGTAGGACGAATGTGTTTG
  GATCAGTATTCCATGTTACCGGCTACTCCATGGGGTGTGTGGGAAATAATCAAGCGAACT
  GGCATTCCAACCCTAGGGAAGAATGTGGTTGTGGCTGGGAAGGTCAAAAAACGTTGGAAT
  GCCCATTGCAATGTTACTGCACACAGATGGGGCGCATGAACGTCCCGGAGGTGATGCCAC
  TGTTACAATTATCTCATCGATAATACTCCCAAAGAGCAGTTGAAGAAACATTACAATTCT
  TGCAGATATTTGTAATATTCTGCTGCAAGTTATTCAAAATCTGATCACAAGCAGATTTGA
  TCAAGGAGGAGCCAGGCAGTCATTGATTGTGGAAAAAATAGAGTTTCACGATTCTGTTTA
  CTTGCCTAAACCAGTTTGTTTGGGAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006636
• ORF Size: 1053 bp
• Insert Size: 2100
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_006636.3, NP_006627.2
• RefSeq Size: 2208
• RefSeq ORF: 1053
• Locus ID: 10797
• Domains: THF_DHG_CYH
• Protein Families: Druggable Genome
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate
• Gene Summary: This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (1) represents the shorter transcript and is the protein-coding transcript.