MCM5 (NM_006739) Human Untagged Clone

CAT#: SC323774

MCM5 (untagged)-Human minichromosome maintenance complex component 5 (MCM5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM5
• Synonyms: CDC46; MGORS8; P1-CDC46
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006739.2 CTTGTCTCCCCTGGTTTGTGAAGTGCGGAAAACCAGAGGCGCAGTCATGTCGGGATTCGA
  CGATCCTGGCATTTTCTACAGCGACAGCTTCGGGGGCGACGCCCAGGCCGACGAGGGGCA
  GGCCCGCAAATCGCAGCTGCAGAGGCGCTTCAAGGAGTTCCTGCGGCAGTACCGAGTGGG
  CACCGACCGCACGGGCTTCACCTTCAAATACAGGGATGAACTCAAGCGGCATTACAACCT
  GGGGGAGTACTGGATTGAGGTGGAGATGGAGGATCTGGCCAGCTTTGATGAGGACCTGGC
  CGACTACTTGTACAAGCAGCCAGCCGAGCACCTGCAGCTGCTGGAGGAAGCTGCCAAGGA
  GGTAGCTGATGAGGTGACCCGGCCCCGGCCTTCTGGGGAGGAGGTGCTCCAGGACATCCA
  GGTCATGCTCAAGTCGGACGCCAGCCCTTCCAGCATTCGTAGCCTGAAGTCGGACATGAT
  GTCACACCTGGTGAAGATCCCTGGCATCATCATCGCGGCCTCTGCGGTCCGTGCCAAGGC
  CACCCGCATCTCTATCCAGTGCCGCAGCTGCCGCAACACCCTCACCAACATTGCCATGCG
  CCCTGGCCTCGAGGGCTATGCCCTGCCCAGGAAGTGCAACACAGATCAGGCTGGGCGCCC
  CAAATGCCCATTGGACCCGTACTTCATCATGCCCGACAAATGCAAATGCGTGGACTTCCA
  GACCCTGAAGCTGCAGGAGCTGCCTGATGCAGTCCCCCACGGGGAGATGCCCAGACACAT
  GCAGCTCTACTGCGACAGGTACCTGTGTGACAAGGTCGTCCCTGGGAACAGGGTTACCAT
  CATGGGCATCTACTCCATCAAGAAGTTTGGCCTGACCACCAGCAGGGGCCGTGACAGGGT
  GGGCGTGGGCATCCGAAGCTCCTACATCCGTGTCCTGGGCATCCAGGTGGACACAGATGG
  CTCTGGCCGCAGCTTTGCTGGGGCCGTGAGCCCCCAGGAGGAGGAGGAGTTCCGTCGCCT
  GGCTGCCCTCCCAAATGTCTATGAGGTCATCTCCAAGAGCATCGCCCCCTCCATCTTTGG
  GGGCACAGACATGAAGAAGGCCATTGCCTGCCTGCTCTTTGGGGGCTCCCGAAAGAGGCT
  CCCTGATGGACTTACTCGCCGAGGAGACATCAACCTGCTGATGCTAGGGGACCCTGGGAC
  AGCCAAGTCCCAGCTTCTGAAGTTTGTGGAGAAGTGTTCTCCCATTGGGGTATACACGTC
  TGGGAAAGGCAGCAGCGCAGCTGGACTGACAGCCTCGGTGATGAGGGACCCTTCGTCCCG
  GAATTTCATCATGGAGGGCGGAGCCATGGTCCTGGCCGATGGTGGGGTCGTCTGTATTGA
  CGAGTTTGACAAGATGCGAGAAGATGACCGTGTGGCAATCCACGAAGCCATGGAGCAGCA
  GACCATCTCTATCGCCAAGGCTGGGATCACCACCACCCTGAACTCCCGCTGCTCCGTCCT
  GGCTGCTGCCAACTCAGTGTTCGGCCGCTGGGATGAGACGAAGGGGGAGGACAACATTGA
  CTTCATGCCCACCATCTTGTCGCGCTTCGACATGATCTTCATCGTCAAGGATGAGCACAA
  TGAGGAGAGGGATGTGATGCTGGCCAAGCATGTCATCACTCTGCACGTGAGCGCACTGAC
  ACAGACACAGGCTGTGGAGGGCGAGATTGACCTGGCCAAGCTGAAGAAGTTTATTGCCTA
  CTGCCGAGTGAAATGTGGCCCCCGGCTGTCAGCAGAGGCTGCAGAGAAACTGAAGAACCG
  CTACATCATCATGCGGAGCGGGGCCCGTCAGCACGAGAGGGACAGTGACCGCCGCTCCAG
  CATCCCCATCACTGTGCGGCAGCTGGAGGCCATTGTGCGCATCGCGGAAGCCCTCAGCAA
  GATGAAGCTGCAGCCCTTCGCCACAGAGGCAGATGTGGAGGAGGCCCTGCGGCTCTTCCA
  AGTGTCCACGTTGGATGCTGCCTTGTCCGGTACCCTGTCAGGGGTGGAGGGCTTCACCAG
  CCAGGAGGACCAGGAGATGCTGAGCCGCATCGAGAAGCAGCTCAAGCGCCGCTTTGCCAT
  TGGCTCCCAGGTGTCTGAGCACAGCATCATCAAGGACTTCACCAAGCAGAAATACCCGGA
  GCACGCCATCCACAAGGTGCTGCAGCTCATGCTGCGGCGCGGCGAGATCCAGCATCGCAT
  GCAGCGCAAGGTTCTCTACCGCCTCAAGTGAGTCGCGCCGCCTCACTGGACTCATGGACT
  CGCCCACGCCTCGCCCCTCCTGCCGCTGCCTGCCATTGACAATGTTGCTGGGACCTCTGC
  CTCCCCACTGCAGCCCTCGAACTTCCCAGGCACCCTCCTTTCTGCCCCAGAGGAAGGAGC
  TGTAGTGTCCTGCTGCCTCTGGGCGCCCGCCTCTAGCGCGGTTCTGGGAAGTGTGCTTTT
  GGCATCCGTTAATAATAAAGCCACGGTGTGTTCAGGTAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006739
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006739.2, NP_006730.2
• RefSeq Size: 2548 bp
• RefSeq ORF: 2205 bp
• Locus ID: 4174
• Cytogenetics: 22q12.3
• Domains: MCM
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: 'The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq, Jul 2008]'