DDX18 (NM_006773) Human Untagged Clone

CAT#: SC323819

DDX18 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX18
• Synonyms: Has1; MrDb
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006773.3 GGGAAGTAACGTCAGCCTGAGAACTGAGTAGCTGTACTGTGTGGCGCCTTATTCTAGGCA
  CTTGTTGGGCAGAATGTCACACCTGCCGATGAAACTCCTGCGTAAGAAGATCGAGAAGCG
  GAACCTCAAATTGCGGCAGCGGAACCTAAAGTTTCAGGGGGCCTCAAATCTGACCCTATC
  GGAAACTCAAAATGGAGATGTATCTGAAGAAACAATGGGAAGTAGAAAGGTTAAAAAATC
  AAAACAAAAGCCCATGAATGTGGGCTTATCAGAAACTCAAAATGGAGGCATGTCTCAAGA
  AGCAGTGGGAAATATAAAAGTTACAAAGTCTCCCCAGAAATCCACTGTATTAAGCAATGG
  AGAAGCAGCAATGCAGTCTTCCAATTCAGAATCAAAAAAGAAAAAGAAGAAAAAGAGAAA
  AATGGTGAATGATGCTGAGCCTGATACGAAAAAAGCAAAAACTGAAAACAAAGGGAAATC
  TGAAGAAGAAAGTGCCGAGACTACTAAAGAAACAGAAAATAATGTGGAGAAGCCAGATAA
  TGATGAAGATGAGAGTGAGGTGCCCAGTCTGCCCCTGGGACTGACAGGAGCTTTTGAGGA
  TACTTCGTTTGCTTCTCTATGTAATCTTGTCAATGAAAACACTCTGAAGGCAATAAAAGA
  AATGGGTTTTACAAACATGACTGAAATTCAGCATAAAAGTATCAGACCACTTCTGGAAGG
  CAGGGATCTTCTAGCAGCTGCAAAAACAGGCAGTGGTAAAACCCTGGCTTTTCTCATCCC
  TGCAGTTGAACTCATTGTTAAGTTAAGGTTCATGCCCAGGAATGGAACAGGAGTCCTTAT
  TCTCTCACCTACTAGAGAACTAGCCATGCAAACCTTTGGTGTTCTTAAGGAGCTGATGAC
  TCACCACGTGCATACCTATGGCTTGATAATGGGTGGCAGTAACAGATCTGCTGAAGCACA
  GAAACTTGGTAATGGGATCAACATCATTGTGGCCACACCAGGCCGTCTGCTGGACCATAT
  GCAGAATACCCCAGGATTTATGTATAAAAACCTGCAGTGTCTGGTTATTGATGAAGCTGA
  TCGTATCTTGGATGTGGGGTTTGAAGAGGAATTAAAGCAAATTATTAAACTTTTGCCAAC
  ACGTAGACAGACTATGCTCTTTTCTGCCACCCAAACTCGAAAAGTTGAAGACCTGGCAAG
  GATTTCTCTGAAAAAGGAGCCATTGTATGTTGGCGTTGATGATGATAAAGCGAATGCAAC
  AGTGGATGGTCTTGAACAGGGATATGTTGTTTGTCCTTCTGAAAAGAGATTCCTTCTGCT
  CTTTACATTCCTTAAGAAGAACCGAAAGAAGAAGCTTATGGTCTTCTTTTCATCTTGTAT
  GTCTGTGAAATACCACTATGAGTTGCTGAACTACATTGATTTGCCCGTCTTGGCCATTCA
  TGGAAAGCAAAAGCAAAATAAGCGTACAACCACATTCTTCCAGTTCTGCAATGCAGATTC
  GGGAACACTATTGTGTACGGATGTGGCAGCGAGAGGACTAGACATTCCTGAAGTCGACTG
  GATTGTTCAGTATGACCCTCCGGATGACCCTAAGGAATATATTCATCGTGTGGGTAGAAC
  AGCCAGAGGCCTAAATGGGAGAGGGCATGCCTTGCTCATTTTGCGCCCAGAAGAATTGGG
  TTTTCTTCGCTACTTGAAACAATCCAAGGTTCCATTAAGTGAATTTGACTTTTCCTGGTC
  TAAAATTTCTGACATTCAGTCTCAGCTTGAGAAATTGATTGAAAAGAATTACTTTCTTCA
  TAAGTCAGCCCAGGAAGCATATAAGTCATACATACGAGCCTATGATTCCCATTCTCTGAA
  ACAGATCTTTAATGTTAATAACCTAAATTTGCCTCAGGTTGCTCTGTCATTTGGTTTCAA
  GGTGCCTCCCTTCGTTGATCTGAACGTCAACAGTAATGAAGGCAAGCAGAAAAAGCGAGG
  AGGTGGTGGTGGATTTGGCTACCAGAAAACCAAGAAAGTTGAGAAATCCAAAATCTTTAA
  ACACATTAGCAAGAAATCATCTGACAGCAGGCAGTTCTCTCACTGAACACATGCCTTCCT
  TTCATCTTGAATAACTTTGTCCTAAAATGAATTTTTTTTCCCCTTGATTTAACAGGATTT
  TTGTAGACTTTAGAATTTGGACTTACCTAACAAGAGTATAAATTGACTTGGGTTGCAAGC
  ACTGAGCACTGTTACTTCTATCACGTCTCTCTTTTATTTCTGGGATATAAAACAGGCTTT
  AAGTTTCTTGGTTGCCCAAGGGCAGAGCAAGGAATATCTGGTGTTTCTTGTGATGATAAT
  ATTTTAATTTTAAATATCCCTCCCTCATACAAGTGTATGTTACCATTTTAATATAATTCT
  TTTTGTACCTTTCCTTCTTGTTTTGTGAAGATTTTTGTGGCATGGATTGCTGTGCTCACT
  GCTGTAAAAGGTGACCTAGTGTACTGGGCAGCTGGTGGCGGTGCAGAAAAGAGTCTCAGG
  TTATTTTTTGTTTTTAGTTATTTCTTGGACCTTGACAGTATCTAATGACTCCTCCTGAAA
  ATGCTGCAGTATAAAAGAGCAAAGAGCTTTGGGAAATACCTAAGAAGCACCTTAAGATTA
  GGGTGGCATTGCTTTTATAGATTCTTGATTTTAAAGCAACAGGCCTTTCTCAGGTGTTGC
  ATTTTTTGGAGCAAAAACTATGGGTTGTAATTTGAATAAAGTGTCACTAAGCAGTTATAA
  CGTTTGATGGCTGGGGGGTAGGAAGAGGATGGAATTGAGATGTTTGAGCCTCATTTACAT
  CAATAGAGGTGTAATGTACTGCATTTCTTCATTTGGTAACATAACAAAGACTTTCATACA
  AAGAACGATGATGCTCCTCATTAAGATTTGTTTAATTCAAGGTGGTTTGGATTTGGTAAG
  CCTTTGCACTCTGTAGAGTACTTAGAAGACAAGGGCAACTTACTTGGAGTTAGAGCCAAG
  CTGTCAGACGGTGCCCAGCACACATTAATGTTAGCTTCTTTCTGAGAAAAAAATACCTCT
  TCCAGGCCCTGAAACAAAAAATACATTTGCTGTGAAGATTGAAAATGAACAAAGTTAGAA
  AAAAAAACAGCAAAATCAGTGATTTAGTCAGATGAGTTTTTCGTTGTAGGAGCACTTGAT
  TTCTAGTGTGTTTTGTACAGTATATAACTACAAGATAGTACATTTTGTAGCAGTTCAAAG
  CCAAAGTTGCTAGCATCATTTTGCTGTTGTGCCAGTTAATCATAGGATCCCATTAAATAA
  GTGTGCTAACATCGAATATAGAGAAAACTGGTAAAGAACATTCCAGTAGGAAAAGAAAAG
  AACAATCTTCCATTTCTGGGCTTGGCCACCATCACCCTGGTCGGACCTGTCCTGGACTTC
  CAACCTTGACTGCTGAGCTCCTGGCTTAGCTTCTTGGGTTCCTAATTCCTGGTGTTTAAT
  AATTCTCTCCACGATCATGTTTTTCTGATTTTTTTTTTCAGAAATAATGTTTTTTAAAAG
  ACAAAAACAAAGGGAAGAATATTTAATTACTGAGCAGAAGTAAATACTGTTGGTATTTTG
  TACATAATCTAATTTTTATATGCATGTTCATGCTTTTTAATTTTTTTATCAAAAATTAAG
  TCATCTACCTACTACTTGTAACCAGCTTGTTTCATAACATGTTATTTTCCTGTGTCATTA
  AATAATTACTTCAATGTTGAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006773.3 unedited
  GGCCTAGGTGGCTAACAACGAACCTCACTACTAGAGGCCGGCACGGCGACTCGCGGCGCG
  CCGGTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGGAAGTAACGTC
  AGCCTGAGAACTGAGTAGCTGTACTGTGTGGCGCCTTATTCTAGGCACTTGTTGGGCAGA
  ATGTCACACCTGCCGATGAAACTCCTGCGTAAGAAGATCGAGAAGCGGAACCTCAAATTG
  CGGCAGCGGAACCTAAAGTTTCAGGGGGCCTCAAATCTGACCCTATCGGAAACTCAAAAT
  GGAGATGTATCTGAAGAAACAATGGGAAGTAGAAAGGTTAAAAAATCAAAACAAAAGCCC
  ATGAATGTGGGCTTATCAGAAACTCAAAATGGAGGCATGTCTCAAGAAGCAGTGGGAAAT
  ATAAAAGTTACAAAGTCTCCCCAGAAATCCACTGTATTAAGCAATGGAGAAGCAGCAATG
  CAGTCTTCCAATTCAGAATCAAAAAAGAAAAAGAAGAAAAAGAGAAAAATGGTGAATGAT
  GCTGAGCCTGATACGAAAAAAGCAAAAACTGAAAACAAAGGGAAATCTGAAGAAGAAAGT
  GCCGAGACTACTAAAGAAACAGAAAATAATGTGGAGAAGCCAGATAATGATGAAGATGAG
  AGTGAGGTGCCCAGTCTGCCCCTGGGACTGACAGGAGCTTTTGAGGATACTTCGTTTGCT
  TCTCTATGTAATCTTGTCAATGAAAACACTCTGAAGGCAATAAAGAATGGGTTTACAAAC
  ATGACTGAAATTCAGCATAAAAGTATCAGACCACTTCTGGAAGGCAGGGATTCTTCTAGC
  AGCTGCAAAACCAGGCAGTGTAAAACCCTGCCTTTTCTTCATCCTTGCAGTTGACTCATT
  GGTTAAGTAAAGTCATGCCAGATATGAACAGGATCCCTAATTCTCCACTCATGAGAACAT
  ACCATCGAACCTTTGTGTATTCTATGAGCCCGGTAGTATACGCACGCTCGGCATACT
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006773
• ORF Size: 2013 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_006773.3, NP_006764.3
• RefSeq Size: 3790
• RefSeq ORF: 2013
• Locus ID: 8886
• Domains: DEAD, helicase_C
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein. [provided by RefSeq, Jul 2008]