ADPRH (NM_001125) Human Untagged Clone

CAT#: SC323885

ADPRH (untagged)-Human ADP-ribosylarginine hydrolase (ADPRH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADPRH
• Synonyms: ARH1; hARH1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001125.2 CAGGTGCGGGGGCCCTCGCCGCCACCCTGCTTATCTGGGGACAGTCCCGGAACCCGGTGA
  CCCGGCTCCGCCCCAACCCGCGCCCTCGTCCACCCGCAGCAGCCAAGGCCTCTTCCCCGG
  CCCCGGGAGCCCGCGCCACGCCCGTCGCTCGCGCGTTCCAGTCCAGGCTACAGCAGGGGC
  TCTCCAGCCTGCATTCCATGGCCAACAGCAGTGCAGCTGCAGAAGCACTTCTGGAAGCCT
  CCTGACTTGAACTAGACCTTGGACACCCTCTCCAGAGCCCAGCAATTGTGAGGGACTGAT
  GGAGAAGTATGTGGCTGCTATGGTGCTGAGTGCAGCTGGAGATGCCCTGGGGTACTACAA
  TGGGAAGTGGGAGTTCCTCCAGGATGGGGAGAAGATACACCGGCAGTTGGCCCAGCTGGG
  CGGCTTGGATGCCCTAGACGTGGGAAGGTGGAGAGTTAGTGACGACACAGTGATGCACTT
  GGCCACAGCAGAAGCTCTTGTGGAAGCTGGGAAAGCCCCTAAGTTGACTCAACTGTATTA
  CCTCCTTGCTAAGCATTACCAAGACTGCATGGAAGACATGGATGGGCGGGCACCAGGTGG
  TGCCTCGGTGCACAACGCCATGCAGCTGAAGCCGGGCAAGCCCAATGGCTGGAGGATTCC
  CTTCAACAGCCATGAGGGCGGCTGTGGGGCTGCCATGCGGGCCATGTGCATCGGTCTCAG
  GTTCCCACACCATAGCCAACTGGACACACTGATCCAAGTGAGCATCGAGAGTGGTCGGAT
  GACCCACCACCACCCAACAGGCTACCTGGGGGCCCTTGCGTCTGCTCTTTTTACAGCCTA
  TGCAGTGAATAGCAGACCACCCTTGCAGTGGGGAAAAGGACTGATGGAGCTGCTACCAGA
  AGCTAAAAAGTACATTGTCCAATCAGGCTACTTTGTAGAGGAAAATCTTCAACACTGGTC
  CTACTTCCAAACCAAATGGGAAAATTACCTAAAACTTAGAGGGATTTTGGATGGAGAATC
  AGCCCCTACCTTCCCTGAGTCTTTCGGTGTGAAGGAGAGGGATCAGTTCTACACCTCCCT
  GAGCTACTCTGGCTGGGGTGGCAGCAGTGGGCACGATGCCCCCATGATTGCCTACGATGC
  TGTTCTTGCTGCAGGAGACTCCTGGAAGGAGCTTGCCCACCGAGCCTTTTTCCATGGTGG
  AGACAGTGATTCTACAGCTGCCATTGCTGGCTGCTGGTGGGGAGTTATGTATGGTTTTAA
  AGGAGTGAGTCCCTCCAACTATGAGAAACTAGAATACAGAAACCGGCTGGAAGAGACAGC
  TAGGGCTTTATATTCTCTCGGGTCAAAAGAAGACACTGTAATTTCCCTTTAGGGAGACGT
  GATGTTCACTTCTGATGGATTCTTCTTTTGTGTATTTCCTTTTCTGCTATTTCTTTTCAG
  TTTTTCCAAAGTCAAGAGTCTTAACCTTGTACTCAGGGAATTTTGAGATAACAAGTCCCT
  TGGGCACCTTAAGCTCAGTTTTTTCAGGCTCATCCTGTTCTTCCAGAATCTATCCCTTTT
  CTTACACTGAAGAGTCCTTTAGCTCAATTGATAGGGTCAGCGTCTCGCAAGCAAAAAATC
  TCATGACCTCATATTTGAGTCTTCATTTTCATCATCTACTTGTTCTAAAAGTTTTTTCTG
  TTTGTCTCAAGTTGGGTTCCCACAAAGTGGACTTTGAGACAAAGATCTGAATGCAAGCAG
  TTTATATGGGAGATGATTCCAGGAAATACCGATATGAGAATGGGGAAATGAAACCAGGAA
  GGGAAGAGAGCTAGTATGGGGTATGTTAATGAGCAGGTTTCCACTGTGGGCGGTTGGCAC
  TCTGCCATCTGAGTCCTCTGCCTAAAGCATTCCTCAGAACTGTCCCATTTGAAGGGCAAG
  CAAACCTGGGGTATTTATCCACTGACTTCCATCTGACACTGGTTGAGGACCACTGCCAGG
  GATGCTAACTCCCTGGTAATTCTTGGCTTACCCTTTGTACAAACCAAACCTTAGTGTCTT
  CAGGTAAAGACTCAGAGATACTTGCAGGGGCAAGCTGTAAAGGGCATGGGAACAGTGAGG
  GACATATGTGGGGTCCTAACAATATCTGCTAGGCTGGTCTACCTCCTAAATATACTCTGA
  ATCTATCACCTCTATTGCCTTCTTGCACTGAGGATTTTTCAGCTGGATTTCTGCAATCCA
  AGCTACTCAAGTCTGGGAGATGCTGCTCTGAGCTGCTCTCAGTACTCTCCTCATCATGGC
  AGTTAACACATTACCAATTTTGTTATTGTCTGTTTGCTTGTTTGCATTATTTCTAAACAG
  AACCTAGCACAGTGCTTGGGCAGCCAATAGGCATTAATCAGTATCTATTGAGTGTTATGA
  ACTAGCCTCCTAGCTCGTGCTTCCCTCATTACCCTCCAGAGTTATGTTTACGAATAACAA
  ATTGACCATGCTAAACTAATCATGTTCCAATGAATACATCACCTTTCTTCATTTCTTGAT
  GCTCTTCCCAATCCTTGTCTACCTGATAATAAATTCTTACTTGTCCTTCAAGACCCAACT
  CAGATAGATAGAGCTTCCTGAGTGAAACCTGCTTTGATTGTTCAGGCACTTGGCCACCTT
  CTCCTCACTCCTCCCACAGCCTTCTGTCTTGGCCTTGTCATCACATCAATCCTGTTGTGT
  GGTGCTGTGTGTCTTCTGGGTCAGTCCTAGGCACATGCTGCCCAGCACATGTCCACCTGG
  GAAATGAGATGCCATCGTCACATTCATTTGGTCACCCTCAGTCCAGCCAGTGGGCTTGCT
  CTCATTTGGCCTGTTCTCAGGGGTGGGAAGGAAGAGCGTCCCTTCCTTCTCCTTTTGAGA
  AAACCACAGAACTATGACTCTCATGATTTTTCCTTTGTGAAATCCCTGGTTTTCAGCTGG
  AAGCGGTGGGTAAATGAGGTCTGATGATAGCAGTCCTGCCACCTTCTAGTTCATCCCATA
  GGAATGTTTTTCCAAGTCCTTCCAGTCTTTTTAGAACGTGGTGGAGGAGGGTTGTGTGTG
  CCCCAGGGGGTCGGGGGTAGGGGATGCAGGGAGGATCTTAGCATTTGTTGTTTTCTAGTG
  CAGGGCTTTAGCCACAATTGTAAACAACAGGAAAAGGCTCTTTAGAGATCCTATTAGATT
  TGCTTTGCCGATTGTCACTCTTCATCACAGGCACATTTCAAAAAGAGGTTAGACTTTCAG
  ACTTCTTTTTTCTCAAACCTCAAAACTTCCACCCCAAACTTCGCTCTCAGCTGGTGACTT
  TGCTTCTTTATGTGTGTGTATGTGTGTCAGAGAAAGAGAGAGAGAGAGAGAGAAGGGAGG
  ATGAGAACGAATGGGGGGAAGAGATAAACTTTATGCATAACATTAGGGTAAATCAATAAA
  GATGGTCTGACACATCGTAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001125
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001125.2, NP_001116.1
• RefSeq Size: 3461 bp
• RefSeq ORF: 1074 bp
• Locus ID: 141
• Cytogenetics: 3q13.33
• Domains: ADP-ribosyl_GH
• Gene Summary: 'The enzyme encoded by this gene catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes that require DTT for maximal activity, the human enzyme is DTT-independent. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.