SF2 (SRSF1) (NM_006924) Human Untagged Clone

CAT#: SC323930

SRSF1 (untagged)-Human serine/arginine-rich splicing factor 1 (SRSF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRSF1
• Synonyms: ASF; SF2; SF2p33; SFRS1; SRp30a
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006924.4 GGCCTGTTCTCGAGTCCGCGCTTTTCGTCACCGCCATGTCGGGAGGTGGTGTGATTCGTG
  GCCCCGCAGGGAACAACGATTGCCGCATCTACGTGGGTAACTTACCTCCAGACATCCGAA
  CCAAGGACATTGAGGACGTGTTCTACAAATACGGCGCTATCCGCGACATCGACCTCAAGA
  ATCGCCGCGGGGGACCGCCCTTCGCCTTCGTTGAGTTCGAGGACCCGCGAGACGCGGAAG
  ACGCGGTGTATGGTCGCGACGGCTATGATTACGATGGGTACCGTCTGCGGGTGGAGTTTC
  CTCGAAGCGGCCGTGGAACAGGCCGAGGCGGCGGCGGGGGTGGAGGTGGCGGAGCTCCCC
  GAGGTCGCTATGGCCCCCCATCCAGGCGGTCTGAAAACAGAGTGGTTGTCTCTGGACTGC
  CTCCAAGTGGAAGTTGGCAGGATTTAAAGGATCACATGCGTGAAGCAGGTGATGTATGTT
  ATGCTGATGTTTACCGAGATGGCACTGGTGTCGTGGAGTTTGTACGGAAAGAAGATATGA
  CCTATGCAGTTCGAAAACTGGATAACACTAAGTTTAGATCTCATGAGGGAGAAACTGCCT
  ACATCCGGGTTAAAGTTGATGGGCCCAGAAGTCCAAGTTATGGAAGATCTCGATCTCGAA
  GCCGTAGTCGTAGCAGAAGCCGTAGCAGAAGCAACAGCAGGAGTCGCAGTTACTCCCCAA
  GGAGAAGCAGAGGATCACCACGCTATTCTCCCCGTCATAGCAGATCTCGCTCTCGTACAT
  AAGATGATTGGTGACACTTTTTGTAGAACCCATGTTGTATACAGTTTTCCTTTATTCAGT
  ACAATCTTTTCATTTTTTAATTCAAACTGTTTTGTTCAGAATGGGCTAAAGTGTTGAATT
  GCATTCTTGTAATATCCCCTTGCTCCTAACATCTACATTCCCTTCGTGTCTTTGATAAAT
  TGTATTTTAAGTGATGTCATAGACAGGATTGTTTAAATTTAGTTAACTCCATACTCTTCA
  GACTGTGATATTGTGTAAATGTCTATCTGCCCTGGTTTGTGTGAACTGGGATGTTGGGGG
  TGTTTGTGGTTATCTTACCTGGGGAAGTTCTTATGTTTATCTTGCTTTTCATGTGTCTTT
  CTGTAGACATATCTGAAGAGATGGATTAAGAATGCTTTGGATTAAGGATTGTGGAGCACA
  TTTCAATCATTTTAGGATTGTCAAAAGGAGGATTGAGGAGGATCAGATCAATAATGGAGG
  CAATGGTATGACTCCAAGTGCTATTGTCACAGATGAAATTGGCAGTATTGACCTTATACT
  AAAAGGCAGGGGTTAAAAATGATTATATACATTTTCCTTAAAACACTTGCAAACATTTTA
  TTCAGTTGTCTTTAGCTACAATTGCTTTGCTTTTTAAACCTTGGCAATTGTGGCAAAATT
  ATATTGCCCATTTTGTAGCAACTTATTTTGCTCCCTTCCCCCCATTTTTGTTTTAATAGG
  GACTAATGTGGGAAGAACTGGCTAATTTGTCACAGTGCTTAGTTACAACTGTTAATGTGT
  GACCTGCTGTTGGTGTACATGTGGGTACAGGGTGTTTTTAAATCCAACAAGATAGAGTAT
  AATATCAATACTGCTAAATCTGCATGTCCTCTGTGTGACTGATAGAGCGTTGCTATTTCA
  TTTTTTTAAGACAAAATGAAAGCAAAATATAGAGTTCCAATGTATTGGTGTAGATAATCT
  AGTTGGGAATACTTTTAAGTCTCACCTTCCCCTTTAAACTAATATTCATAATTGGTTCAT
  ATGTTTAAAAGACTTTAATTTACAAATTAAATTGCAAATGGGAGCATTAGATTTAGTTTT
  AGACTTAGGTGGGTAGCAATGCCAGTAAACTTAAATTACGTAACTTCTTGCAACCACGAA
  ACCTGTAATACGCTGTACAGTAACAAGTGTTGGCATTATCAGTTGAACTGTAAATACAAA
  ATGCTTCTTCCAATTAGTCTCTATGATGATTAAGTTTCTAAAATTTATCTGAACACCATT
  CAGAAACTTGTTTTGGGGAATTTGATAGTTATTGATGTGCATCTGTTAAACTGATGACAG
  ACATAACTCATCATTCCCCAGAAACCTTTTTTGATTACAGTATCTAACATTTTGCCTCCT
  CTTTTTTGGTTTTGCTGGTTATAAAGGTTTGGATTGGAGAGGGCTCACTGGATCCCAATC
  CTTGGAGCTGGATCATTGGATTCAAATCATAATGTGGATAGGATAGGGAGGATGAATTAC
  CAGGATTCATGGAGCGGGATCAGATTACCAGGAACATAGGAGTGGATTCCTGCCCCAACC
  AAACCGCATTCGTGTGGATTTTTTTATTCAACTTAATTGGCTATTCCAAAGATTTTTTTT
  TTCCTATTTTTGACGATTGGAGCCCTTAAGATGCACGATGGAATTGTGTTTTGCGTTTTT
  TGGTAAAAGGAGCAAAGCGAGGACCTGGAGATAAACGCTGGAGCAATCTCCTTGGAAGGA
  TTCAGCACGAGTAGATGGTAAACATTTAAAGGGGAAAGGGGGGGTTTGTTTAAAATAGTA
  AATCAGTAAGTCACTTCTAAATTTAAAGAAAACAAAATTGGAGTTGAAGAATAAGTAGGT
  TTCCAATTGGCTATTGCCGTTTTCTTTGAAAAAATAAACATTTTTTAAAAAACAAAAAAA
  AAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006924
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006924.4, NP_008855.1
• RefSeq Size: 5468 bp
• RefSeq ORF: 747 bp
• Locus ID: 6426
• Cytogenetics: 17q22
• Domains: RRM
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Spliceosome
• Gene Summary: 'This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014]'
  Transcript Variant: This variant (1) encodes the longer isoform (1, also known as ASF-1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.