SF2 (SRSF1) (NM_006924) Human Untagged Clone

CAT#: SC323930

SRSF1 (untagged)-Human serine/arginine-rich splicing factor 1 (SRSF1), transcript variant 1


  "NM_006924" in other vectors (7)

Reconstitution Protocol

USD 420.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SRSF1
Synonyms ASF; SF2; SF2p33; SFRS1; SRp30a
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_006924.4 GGCCTGTTCTCGAGTCCGCGCTTTTCGTCACCGCCATGTCGGGAGGTGGTGTGATTCGTG
GCCCCGCAGGGAACAACGATTGCCGCATCTACGTGGGTAACTTACCTCCAGACATCCGAA
CCAAGGACATTGAGGACGTGTTCTACAAATACGGCGCTATCCGCGACATCGACCTCAAGA
ATCGCCGCGGGGGACCGCCCTTCGCCTTCGTTGAGTTCGAGGACCCGCGAGACGCGGAAG
ACGCGGTGTATGGTCGCGACGGCTATGATTACGATGGGTACCGTCTGCGGGTGGAGTTTC
CTCGAAGCGGCCGTGGAACAGGCCGAGGCGGCGGCGGGGGTGGAGGTGGCGGAGCTCCCC
GAGGTCGCTATGGCCCCCCATCCAGGCGGTCTGAAAACAGAGTGGTTGTCTCTGGACTGC
CTCCAAGTGGAAGTTGGCAGGATTTAAAGGATCACATGCGTGAAGCAGGTGATGTATGTT
ATGCTGATGTTTACCGAGATGGCACTGGTGTCGTGGAGTTTGTACGGAAAGAAGATATGA
CCTATGCAGTTCGAAAACTGGATAACACTAAGTTTAGATCTCATGAGGGAGAAACTGCCT
ACATCCGGGTTAAAGTTGATGGGCCCAGAAGTCCAAGTTATGGAAGATCTCGATCTCGAA
GCCGTAGTCGTAGCAGAAGCCGTAGCAGAAGCAACAGCAGGAGTCGCAGTTACTCCCCAA
GGAGAAGCAGAGGATCACCACGCTATTCTCCCCGTCATAGCAGATCTCGCTCTCGTACAT
AAGATGATTGGTGACACTTTTTGTAGAACCCATGTTGTATACAGTTTTCCTTTATTCAGT
ACAATCTTTTCATTTTTTAATTCAAACTGTTTTGTTCAGAATGGGCTAAAGTGTTGAATT
GCATTCTTGTAATATCCCCTTGCTCCTAACATCTACATTCCCTTCGTGTCTTTGATAAAT
TGTATTTTAAGTGATGTCATAGACAGGATTGTTTAAATTTAGTTAACTCCATACTCTTCA
GACTGTGATATTGTGTAAATGTCTATCTGCCCTGGTTTGTGTGAACTGGGATGTTGGGGG
TGTTTGTGGTTATCTTACCTGGGGAAGTTCTTATGTTTATCTTGCTTTTCATGTGTCTTT
CTGTAGACATATCTGAAGAGATGGATTAAGAATGCTTTGGATTAAGGATTGTGGAGCACA
TTTCAATCATTTTAGGATTGTCAAAAGGAGGATTGAGGAGGATCAGATCAATAATGGAGG
CAATGGTATGACTCCAAGTGCTATTGTCACAGATGAAATTGGCAGTATTGACCTTATACT
AAAAGGCAGGGGTTAAAAATGATTATATACATTTTCCTTAAAACACTTGCAAACATTTTA
TTCAGTTGTCTTTAGCTACAATTGCTTTGCTTTTTAAACCTTGGCAATTGTGGCAAAATT
ATATTGCCCATTTTGTAGCAACTTATTTTGCTCCCTTCCCCCCATTTTTGTTTTAATAGG
GACTAATGTGGGAAGAACTGGCTAATTTGTCACAGTGCTTAGTTACAACTGTTAATGTGT
GACCTGCTGTTGGTGTACATGTGGGTACAGGGTGTTTTTAAATCCAACAAGATAGAGTAT
AATATCAATACTGCTAAATCTGCATGTCCTCTGTGTGACTGATAGAGCGTTGCTATTTCA
TTTTTTTAAGACAAAATGAAAGCAAAATATAGAGTTCCAATGTATTGGTGTAGATAATCT
AGTTGGGAATACTTTTAAGTCTCACCTTCCCCTTTAAACTAATATTCATAATTGGTTCAT
ATGTTTAAAAGACTTTAATTTACAAATTAAATTGCAAATGGGAGCATTAGATTTAGTTTT
AGACTTAGGTGGGTAGCAATGCCAGTAAACTTAAATTACGTAACTTCTTGCAACCACGAA
ACCTGTAATACGCTGTACAGTAACAAGTGTTGGCATTATCAGTTGAACTGTAAATACAAA
ATGCTTCTTCCAATTAGTCTCTATGATGATTAAGTTTCTAAAATTTATCTGAACACCATT
CAGAAACTTGTTTTGGGGAATTTGATAGTTATTGATGTGCATCTGTTAAACTGATGACAG
ACATAACTCATCATTCCCCAGAAACCTTTTTTGATTACAGTATCTAACATTTTGCCTCCT
CTTTTTTGGTTTTGCTGGTTATAAAGGTTTGGATTGGAGAGGGCTCACTGGATCCCAATC
CTTGGAGCTGGATCATTGGATTCAAATCATAATGTGGATAGGATAGGGAGGATGAATTAC
CAGGATTCATGGAGCGGGATCAGATTACCAGGAACATAGGAGTGGATTCCTGCCCCAACC
AAACCGCATTCGTGTGGATTTTTTTATTCAACTTAATTGGCTATTCCAAAGATTTTTTTT
TTCCTATTTTTGACGATTGGAGCCCTTAAGATGCACGATGGAATTGTGTTTTGCGTTTTT
TGGTAAAAGGAGCAAAGCGAGGACCTGGAGATAAACGCTGGAGCAATCTCCTTGGAAGGA
TTCAGCACGAGTAGATGGTAAACATTTAAAGGGGAAAGGGGGGGTTTGTTTAAAATAGTA
AATCAGTAAGTCACTTCTAAATTTAAAGAAAACAAAATTGGAGTTGAAGAATAAGTAGGT
TTCCAATTGGCTATTGCCGTTTTCTTTGAAAAAATAAACATTTTTTAAAAAACAAAAAAA
AAAAAAAA
Restriction Sites ECoRI-NOT     
ACCN NM_006924
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006924.4, NP_008855.1
RefSeq Size 5468 bp
RefSeq ORF 747 bp
Locus ID 6426
Cytogenetics 17q22
Domains RRM
Protein Families Stem cell - Pluripotency
Protein Pathways Spliceosome
Gene Summary 'This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014]'
Transcript Variant: This variant (1) encodes the longer isoform (1, also known as ASF-1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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