GEF H1 (ARHGEF2) (NM_004723) Human Untagged Clone

CAT#: SC323957

ARHGEF2 (untagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGEF2
• Synonyms: GEF; GEF-H1; GEFH1; LFP40; NEDMHM; P40
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004723.2 GAGACCAACGCGTGCGGGCCGAACCCCTCCCCCCGCCTTCCCCCAACAATACAGGACGCC
  GGGGTCCGCGCCGCGTCCTCCCTGGTCCCCCCGTCCGATTATGTCTCGGATCGAATCCCT
  CACGCGGGCGCGGATCGACCGGAGCAGAGAGCTGGCGAGCAAGACCCGGGAAAAGGAGAA
  GATGAAGGAAGCCAAGGATGCCCGCTATACCAATGGGCACCTCTTCACCACCATTTCAGT
  TTCAGGCATGACCATGTGCTATGCCTGTAACAAGAGCATCACAGCCAAGGAAGCCCTCAT
  CTGCCCAACCTGCAATGTGACTATCCACAACCGCTGTAAAGACACCCTCGCCAACTGTAC
  CAAGGTCAAGCAGAAGCAACAGAAAGCGGCCCTGCTGAAGAACAACACCGCCTTGCAGTC
  CGTTTCTCTTCGAAGTAAGACAACCATCCGGGAGCGGCCAAGCTCGGCCATCTACCCCTC
  CGACAGCTTCCGGCAGTCCCTCCTGGGCTCCCGCCGTGGCCGCTCCTCCTTGTCTTTAGC
  CAAGAGTGTTTCTACCACCAACATTGCTGGACATTTCAATGATGAGTCTCCCCTGGGGCT
  GCGCCGGATCCTCTCACAGTCCACAGACTCCCTCAACATGCGGAACCGAACCCTATCCGT
  GGAATCCCTCATTGACGAAGCAGAGGTAATCTACAGTGAGCTGATGAGTGACTTTGAGAT
  GGATGAGAAGGACTTTGCAGCTGACTCTTGGAGTCTTGCTGTGGACAGCAGCTTCCTGCA
  GCAGCATAAAAAGGAGGTGATGAAGCAGCAAGATGTCATCTATGAGCTAATCCAGACAGA
  GCTGCACCATGTGAGGACACTGAAGATCATGACCCGCCTCTTCCGCACGGGGATGCTGGA
  AGAGCTACACTTGGAGCCAGGAGTGGTCCAGGGCCTGTTCCCCTGCGTGGACGAGCTCAG
  TGACATCCATACACGCTTCCTCAGCCAGCTATTAGAACGCCGACGCCAGGCCCTGTGCCC
  TGGCAGCACCCGGAACTTTGTCATCCATCGCTTGGGTGATCTGCTCATCAGCCAGTTCTC
  AGGTCCTAGTGCGGAGCAGATGTGTAAGACCTACTCGGAGTTCTGCAGCCGCCACAGCAA
  GGCCTTAAAGCTCTATAAGGAGCTGTACGCCCGAGACAAACGCTTCCAGCAATTCATCCG
  GAAAGTGACCCGCCCCGCCGTGCTCAAGCGGCACGGGGTACAGGAGTGCATCCTGCTGGT
  GACTCAGCGCATCACCAAGTACCCGTTACTCATCAGCCGCATCCTGCAGCATTCCCACGG
  GATCGAGGAGGAGCGCCAGGACCTGACCACAGCACTGGGGCTAGTGAAGGAGCTGCTGTC
  CAATGTGGACGAGGGTATTTATCAGCTGGAGAAAGGGGCCCGTCTGCAGGAGATCTACAA
  CCGCATGGACCCTCGGGCCCAAACCCCAGTGCCTGGCAAGGGCCCCTTTGGCCGAGAGGA
  ACTTCTGAGGCGCAAACTCATCCACGATGGCTGCCTGCTCTGGAAGACAGCGACGGGGCG
  CTTCAAAGATGTGCTAGTGCTGCTGATGACAGATGTACTGGTGTTTCTCCAGGAAAAGGA
  CCAGAAGTACATCTTTCCTACCCTGGACAAGCCTTCAGTGGTATCGCTGCAGAATCTAAT
  CGTACGAGACATTGCCAACCAGGAGAAAGGGATGTTTCTGATCAGCGCAGCCCCACCTGA
  GATGTACGAGGTGCACACAGCATCCCGGGATGACCGGAGCACCTGGATCCGGGTCATTCA
  GCAGAGCGTGCGCACATGCCCATCCAGGGAGGACTTCCCCCTGATTGAGACAGAGGATGA
  GGCTTACCTGCGGCGAATTAAGATGGAGTTGCAGCAGAAGGACCGGGCACTGGTGGAGCT
  GCTGCGAGAGAAGGTCGGGCTGTTTGCTGAGATGACCCATTTCCAGGCCGAAGAGGATGG
  TGGCAGTGGGATGGCCCTGCCCACCCTGCCCAGGGGCCTTTTCCGCTCTGAGTCCCTTGA
  GTCCCCTCGTGGCGAGCGGCTGCTGCAGGATGCCATCCGTGAGGTGGAGGGTCTGAAAGA
  CCTGCTGGTGGGGCCAGGAGTGGAACTGCTCTTGACACCCCGAGAGCCAGCCCTGCCCTT
  GGAACCAGACAGCGGTGGTAACACGAGTCCTGGGGTCACTGCCAATGGTGAGGCCAGAAC
  CTTCAATGGCTCCATTGAACTCTGCAGAGCTGACTCAGACTCTAGCCAGAGGGATCGAAA
  TGGAAATCAGCTGAGATCACCGCAAGAGGAGGCGTTACAGCGATTGGTCAATCTCTATGG
  ACTTCTACATGGCCTACAGGCAGCTGTGGCCCAGCAGGACACTCTGATGGAAGCCCGGTT
  CCCTGAGGGCCCTGAGCGGCGGGAGAAGCTGTGCCGAGCCAACTCTCGGGATGGGGAGGC
  TGGCAGGGCTGGGGCTGCCCCTGTGGCCCCTGAAAAGCAGGCCACGGAACTGGCATTACT
  GCAGCGGCAACATGCGCTGCTGCAGGAGGAGCTACGGCGCTGCCGGCGGCTAGGTGAAGA
  ACGGGCAACCGAAGCTGGCAGCCTGGAGGCCCGGCTCCGGGAGAGTGAGCAGGCCCGGGC
  ACTGCTGGAGCGTGAGGCCGAAGAGGCTCGAAGGCAGCTGGCCGCCCTGGGCCAGACCGA
  GCCACTCCCAGCTGAGGCCCCCTGGGCCCGCAGACCTGTGGATCCTCGGCGGCGCAGCCT
  CCCCGCAGGCGATGCCCTGTACTTGAGTTTCAACCCCCCACAGCCCAGCCGAGGCACTGA
  CCGCCTGGATCTACCTGTCACTACTCGCTCTGTCCATCGAAACTTTGAGGACCGAGAGAG
  GCAGGAACTGGGGAGCCCCGAAGAGCGGCTGCAAGACAGCAGTGACCCTGACACTGGCAG
  CGAGGAGGAAGGTAGCAGCCGTCTGTCTCCGCCCCACAGTCCACGAGACTTTACCAGAAT
  GCAGGACATCCCGGAGGAGACGGAGAGCCGCGACGGGGAGGCTGTAGCCTCCGAGAGCTA
  AGGGGGCCCCTCCCCCCTGCCCCGTGCCCCACTGAAGAACATTACTGAGGGGGCTAACCT
  TGGGGACTCCAATTTGCCAATGATGAGGGAACATTTGAAAGAACTGCAAATTGTCCTTGC
  CAGCTCTTGGGATCCTTGGATACCTGGGGCCATTTAAGAAGCTAGGGGAATTAGGCCACA
  ACACCCCCTGGGACATCCGAAAGCTACACCACAGATGCCAGTGGTTCATGCCTTCTTCCC
  GCAACTTTAGGAAAATTTATTTATTTATTGTTTATTAGTTATGGGGGGAGAGGGGAGATT
  TAAAGGACCAGGGACATGGGAACCAAGCCATAGGGATCAGAGGGCCTTGTCCTTGAACAC
  TACTGGGGTATATTCAGGCTCATCCACGCAGCTGCTGGGTTCTTGCCCTAACGGCCCTCC
  CCTGCAACATCCGTCTTGGAGGAGAGGCTGCAGCCACAGCACCCTACTGCCCTTTAAATA
  AAGGAGGGCTGTGGGCAGGGCCATGTCCCTTTCTCCTCTCCCCTCAACCTCTTACTGCTG
  TTCTCCCTTTCTCCGTCCTTCATGGAAGCCCTGGGAGATAACCTGGCTTCCTGGAGTTGA
  TGGAATAAAGGTTGGGGTGGCCATAATGGTTTGTTGGGGGTGAGGGAAAAAACCCACAGG
  GACCAGAATGTTTTGTTGTTCTTTTGTTTTCTTTTTTGTACCAAAGTCAACTGCACGTGT
  TTTTATATTTTTAAGAGATCGTAGGCAATTAGAGATCGAAGCCTCCTATCTCCACATCTC
  TGAAGAAGTTGAGGGGTGGGGGAGAGAATGACTTCTGCCTTCATCTGCAGTAACGGGGGG
  ACCTATACTGACCTCTTCCCCAGCCATTTAGAAACAAGTTCTAGGGTGGGTTGGAAAATC
  TCCAAGAGCCCTGACCTCATCTTCCACCTCAGCAACCATGACCTGAAACCTCAGCGTGAA
  TTTGGGGGATTTTTCAGTGGAACCCTTGCCCCCAAATGTCGACCAGCCCCCAAATGTCGA
  AGAATTTTCTTCTTGCCAATTTTGTTGTTTAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004723.2 unedited
  CTCCATAGATGTTAAACGACATCCATCTAGGGCGGCCGGCGATCGCGGCGCGCCGGTACC
  GGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGAGACCAACGCGTGCGGGCCG
  AACCCCTCCCCCCGCCTTCCCCCAACAATACAGGACGCCGGGGTCCGCGCCGCGTCCTCC
  CTGGTCCCCCCGTCCGATTATGTCGCGGATCGAATCCCTCACGCGGGCGCGGATCGACCG
  GAGCAGAGAGCTGGCGAGCAAGACCGGGGAAAAGGAGAAATGAAGGAAGCCAAGGATGCC
  CGCTATACCAATGGGCACCTCTTCAACACAATTTCGGTTTAAGCCGGGGGGGGGGGTATG
  CCTGTACAAAAGTAGCACAGCCAGGGAAGCCATCATCTACCGGAACAGAATGTTAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004723
• ORF Size: 2877 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_004723.2, NP_004714.2
• RefSeq Size: 4093
• RefSeq ORF: 2877
• Locus ID: 9181
• Domains: RhoGEF, PH, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: Pathogenic Escherichia coli infection
• Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2009]
  Transcript Variant: This variant (3) has an alternate 5' exon, which results in a downstream AUG start codon, as compared to variant 1. The resulting isoform (3) has a shorter N-terminus, as compared to isoform 1.