PPM1B (NM_177969) Human Untagged Clone

CAT#: SC324040

PPM1B (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPM1B
• Synonyms: PP2C-beta; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_177969.2 TGCTCAATGGCGGAAAAGCCGCCGGTGCTCTGACGGCCTCGTTCCCCTAGCAGTTGCGGG
  GGAGTTTCCTGCCGGCGCGGCTGGAGTCTCTGATTCTCAGGGTTCGGTGGTTGGAAGATG
  CTCCAGAGAGACGAGGCTGCGGCGGAGGAGGTGGCGGCGGCCGAATCGGCAACGGCGCTA
  GGGTGGAGAGAAGGCGGCAGCGGCGGCGGCGGCGGCGTGAGGGGCCGGGCGGTGTAAACA
  GCCCCGGAGGCGGCGGAGGCGGCGGTCGAGACCCCGAGGGGGAAGCGGCGGCTGAGTCAG
  GGTCGCGCCTCCGTTGGAAACTTGGGCTGAGTACCGCGGCGGGCGCGAGCGAGGCGCCCT
  AGACATCTTCTCCCTCCCTTGCCTCAGATTTATTGCTAAACATGGGTGCATTTTTGGATA
  AACCCAAAACTGAAAAACATAATGCTCATGGTGCTGGGAATGGTTTACGTTATGGCCTGA
  GCAGCATGCAAGGATGGAGAGTGGAAATGGAAGATGCACACACAGCTGTTGTAGGTATTC
  CTCACGGCTTGGAAGACTGGTCATTTTTTGCAGTTTATGATGGTCATGCTGGATCCCGAG
  TGGCAAATTACTGCTCAACACATTTATTAGAACACATCACTACTAACGAAGACTTTAGGG
  CAGCTGGAAAATCAGGATCTGCTCTTGAGCTTTCAGTGGAAAATGTTAAGAATGGTATCA
  GAACTGGATTTTTGAAAATTGATGAATACATGCGTAACTTTTCAGACCTCAGAAACGGGA
  TGGACAGGAGTGGTTCAACTGCAGTGGGAGTTATGATTTCACCTAAGCATATCTACTTTA
  TCAACTGTGGTGATTCACGTGCTGTTCTGTATAGGAATGGACAAGTCTGCTTTTCTACCC
  AGGATCACAAACCTTGCAATCCAAGGGAAAAGGAGCGAATCCAAAATGCAGGAGGCAGCG
  TGATGATACAACGTGTTAATGGTTCATTAGCAGTATCTCGTGCTCTGGGGGACTATGATT
  ACAAGTGTGTTGATGGCAAGGGCCCAACAGAACAACTTGTTTCTCCAGAGCCTGAGGTTT
  ATGAAATTTTAAGAGCAGAAGAGGATGAATTTATCATCTTGGCTTGTGATGGGATCTGGG
  ATGTTATGAGTAATGAGGAGCTCTGTGAATATGTTAAATCTAGGCTTGAGGTATCTGATG
  ACCTGGAAAATGTGTGCAATTGGGTAGTGGACACTTGTTTACACAAGGGAAGTCGAGATA
  ACATGAGTATTGTACTAGTTTGCTTTTCAAATGCTCCCAAGGTCTCAGATGAAGCGGTGA
  AAAAAGATTCAGAGTTGGATAAGCACTTGGAATCACGGGTTGAAGAGATTATGGAGAAGT
  CTGGCGAGGAAGGAATGCCTGATCTTGCCCATGTCATGCGCATCTTGTCTGCAGAAAATA
  TCCCAAATTTGCCTCCTGGGGGAGGTCTTGCTGGCAAGCGTAATGTTATTGAAGCTGTTT
  ATAGTAGACTGAATCCACATAGAGAAAGTGATGGGGCCTCCGATGAAGCAGAGGAAAGTG
  GATCACAGGGAAAATTGGTGGAAGCTCTCAGGCAAATGAGAATTAATCATAGGGGAAACT
  ACCGACAACTTCTGGAGGAGATGCTGACTAGTTACAGGCTAGCTAAAGTAGAGGGAGAAG
  AAAGCCCTGCTGAACCAGCTGCCACAGCTACTTCTTCGAACAGTGATGCTGGAAACCCAG
  TGACAATGCAGGAAAGCCATACTGAATCAGAAAGTGGTCTTGCTGAATTAGACAGCTCTA
  ATGAAGATGCAGGGACAAAGATGAGTGGTGAAAAAATATGACTTTCCTTTTTGGTAATAT
  TTTTGTGATCTTTGATGGTTTTTAACCTAGGAAGTGTAATGTATGCATTTATATAACTGT
  TTTGTTATTTGAATCTTGGAAAACTAGTTTTATTATATTCAGATAGCCTTGTTTTTTAAA
  AAGGCCTTTGCATACACCTTTATGAGATAGTGTAAAATTGACTATTTATAGTACTATGGA
  TTTAATGAAATTATATGTCATTTCACATTGTATGCCAGAAATTAGGCTACCAATTATGAA
  TTAAAGTCAGTAGTTAAATTAATACTAGATAGAATTAGAAATTTTGATTAGAGAGATTAT
  GCTATATTATGGAAAAACTTGTTAATGTAGAATTATACTGCTTCATATTATTTTACCTAT
  TAGTACACTCATAGTTAGCTTTGTAATAAATTTATGTTTTCTTTAATAATTTTAGTTCTT
  CAAAGAATGGCTGATGCTGGCCTGTAATTTTTCTTTCAAGGATGATAATTTGTGTGTTGT
  TTGATTTGTTTATATTTTACATCTCTGTAGTTTTATTTTTAGAAGTTGTGAGATATTGGA
  TGTGTGGCTATTTTTCCTTTCTCTGTATTCTTTATGAAACATAACTTTTGAAAAACCTAT
  GTATTATTCATACAGCTTTGGTTTGTATATTCTGTATAGCCTAACTACACACATCAAAAT
  GTATGTCAACCAAGTGTTTAGAATGAAATTATAAGTGTTTAAGTCCAAATAAAGCATGTG
  ATGTGGAATAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_177969
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177969.2, NP_808908.1
• RefSeq Size: 1776 bp
• RefSeq ORF: 579 bp
• Locus ID: 5495
• Cytogenetics: 2p21
• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) lacks an alternate exon, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) is shorter at the N-terminus, compared to isoform 1.