PPM1B (NM_177969) Human Untagged Clone

CAT#: SC121298

PPM1B (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPM1B
• Synonyms: PP2C-beta; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_177969, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTATTGTACTAGTTTGCTTTTCAAATGCTCCCAAGGTCTCAGATGAAGCGGTGAAAAAAGATTCAG
  AGTTGGATAAGCACTTGGAATCACGGGTTGAAGAGATTATGGAGAAGTCTGGCGAGGAAGGAATGCCTGA
  TCTTGCCCATGTCATGCGCATCTTGTCTGCAGAAAATATCCCAAATTTGCCTCCTGGGGGAGGTCTTGCT
  GGCAAGCGTAATGTTATTGAAGCTGTTTATAGTAGACTGAATCCACATAGAGAAAGTGATGGGGCCTCCG
  ATGAAGCAGAGGAAAGTGGATCACAGGGAAAATTGGTGGAAGCTCTCAGGCAAATGAGAATTAATCATAG
  GGGAAACTACCGACAACTTCTGGAGGAGATGCTGACTAGTTACAGGCTAGCTAAAGTAGAGGGAGAAGAA
  AGCCCTGCTGAACCAGCTGCCACAGCTACTTCTTCGAACAGTGATGCTGGAAACCCAGTGACAATGCAGG
  AAAGCCATACTGAATCAGAAAGTGGTCTTGCTGAATTAGACAGCTCTAATGAAGATGCAGGGACAAAGAT
  GAGTGGTGAAAAAATATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177969 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGG
  CACGAGGGCCCAACAGAACAACTTGTTTCTCCAGAGCCTGAGGTTTATGAAATTTTAAGA
  GCAGAAGAGGATGAATTTATCATCTTGGCTTGTGATGGGATCTGGGATGTTATGAGTAAT
  GAGGAGCTCTGTGAATATGTTAAATCTAGGCTTGAGGTATCTGATGACCTGGAAAATGTG
  TGCAATTGGGTAGTGGACACTTGTTTACACAAGGGAAGTCGAGATAACATGAGTATTGTA
  CTAGTTTGCTTTTCAAATGCTCCCAAGGTCTCAGATGAAGCGGTGAAAAAAGATTCAGAG
  TTGGATAAGCACTTGGAATCACGGGTTGAAGAGATTATGGAGAAGTCTGGCGAGGAAGGA
  ATGCCTGATCTTGCCCATGTCATGCGCATCTTGTCTGCAGAAAATATCCCAAATTTGCCT
  CCTGGGGGAGGTCTTGCTGGCAAGCGTAATGTTATTGAAGCTGTTTATAGTAGACTGAAT
  CCACATAGAGAAAGTGATGGGGGTGCTGGAGATCTANAAGACCCATGGTAGCCTTAAAAA
  CCTTCTAAAATGCTTTTGATTCTGAAAATTGGGGGAAAAAACTTTTAATCACAATTTTCT
  TCAATACAAGGGGAAAATATTCTTGCGGATTCCCAACGTTNTGTGATATGAGCAGANAAT
  CATTAGCATTTCCCATCATTTGTTCATATTNTGNNGTTTCTGACAGTTGCCACTTGTAGC
  ATTGCCTGTACTACAGNTATTTNTTGCCAACCTCANGCATACTCGTTACATCTGTATTGA
  AACTTTCGCCCTAGAACCCANGGGAGNTATTTCACCACAATCAACATGTGCCCTGAGTGC
  ATGGGAAATATAGNTAGCTATCTCTGAATACATNTTGGTTT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_177969
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177969.1, NP_808908.1
• RefSeq Size: 1760 bp
• RefSeq ORF: 579 bp
• Locus ID: 5495
• Cytogenetics: 2p21
• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) lacks an alternate exon, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) is shorter at the N-terminus, compared to isoform 1.