RAD17 (NM_002873) Human Untagged Clone

CAT#: SC324090

RAD17 (untagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 8

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD17
• Synonyms: CCYC; HRAD17; R24L; RAD17SP; RAD24
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002873.1 TTGCTGTCGAAAGTTTTACTATAATGAAAGATATTTTCATACTCTCAAAAATATAGAGGA
  AAGGGGCCAAGATTATAGTACCAGTCACAATCTTTTGATGAGGACGAAATGAATCAGGTA
  ACAGACTGGGTTGACCCATCATTTGATGATTTTCTAGAGTGTAGTGGCGTCTCTACTATT
  ACTGCCACATCATTAGGTGTGAATAACTCAAGTCATAGAAGAAAAAATGGGCCTTCTACA
  TTAGAAAGCAGCAGATTTCCAGCGAGAAAAAGAGGAAATCTATCTTCCTTAGAACAGATT
  TATGGTTTAGAAAATTCAAAAGAATATCTGTCTGAAAATGAACCATGGGTGGATAAATAT
  AAACCAGAAACTCAGCATGAACTTGCTGTGCATAAAAAGAAAATTGAAGAAGTCGAAACC
  TGGTTAAAAGCTCAAGTTTTAGAAAGGCAACCAAAACAGGGTGGATCTATTTTATTAATA
  ACAGGTCCTCCTGGATGTGGAAAGACAACGACCTTAAAAATACTATCAAAGGAGCATGGT
  ATTCAAGTACAAGAGTGGATTAATCCAGTTTTACCAGACTTCCAAAAAGATGATTTCAAG
  GGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGTCTCAGATAGCAGTT
  TTCAAAGAGTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAATGCTTGGAGATGAT
  CTGAGAACTGATAAGAAGATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATCGGGAT
  TCTCATACTTTACATGAAGTTCTAAGGAAGTATGTGAGGATTGGTCGATGTCCTCTTATA
  TTTATAATCTCGGACAGTCTCAGTGGAGATAATAATCAAAGGTTATTGTTTCCCAAAGAA
  ATTCAGGAAGAGTGTTCTATCTCAAATATTAGTTTCAACCCTGTGGCACCAACAATTATG
  ATGAAATTTCTTAATCGAATAGTGACTATAGAAGCTAACAAGAATGGAGGAAAAATTACT
  GTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCAGGGATGTTCTGGTGATATCAGAAGT
  GCAATAAACAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAACTTACGGCCAAGGAAA
  AAAGGAATGTCTTTAAAATCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAAAAACCT
  GATAGGGTTTTTGAAAATCAAGAGGTCCAAGCTATTGGTGGCAAAGATGTTTCTCTGTTT
  CTCTTCAGAGCTTTGGGGAAAATTCTATATTGTAAAAGAGCATCTTTAACAGAATTAGAC
  TCACCTCGGTTGCCCTCTCATTTATCAGAATATGAACGGGATACATTACTTGTTGAACCT
  GAGGAGGTAGTAGAAATGTCACACATGCCTGGAGACTTATTTAATTTATATCTTCACCAA
  AACTACATAGATTTCTTCATGGAAATTGATGATATTGTGAGAGCCAGTGAATTTCTGAGT
  TTTGCAGATATCCTCAGTGGTGACTGGAATACACGCTCTTTACTCAGGGAATATAGCACA
  TCTATAGCTACGAGAGGTGTGATGCATTCCAACAAAGCCCGAGGATATGCTCATTGCCAA
  GGAGGAGGATCAAGTTTTCGACCCTTGCACAAACCTCAGTGGTTTCTAATAAATAAAAAG
  TATCGGGAAAATTGCCTGGCAGCAAAAGCACTTTTTCCTGACTTCTGCCTACCAGCTTTA
  TGCCGCCAAACTCAGCTATTGCCATACCTTGCTCTACTAACCATTCCAATGAGAAATCAA
  GCTCAGATTTCTTTTATCCAAGATATTGGAAGGCTCCCTCTGAAGCGACACTTTGGAAGA
  TTGAAAATGGAAGCCCTGACTGACAGGGAACATGGAATGATAGACCCTGACAGCGGAGAT
  GAAGCCCAGCTTAATGGAGGACATTCTGCAGAGGAATCTCTGGGTGAACCCACTCAAGCC
  ACTGTGCCGGAAACCTGGTCTCTTCCTTTGAGTCAGAATAGTGCCAGTGAACTGCCTGCT
  AGCCAGCCCCAGCCCTTTTCAGCCCAAGGAGACATGGAAGAAAACATAATAATAGAAGAC
  TACGAGAGTGATGGGACATAGAAGCCAGCCTGCTAATCAGATTGCTACTTCACAGCTTCA
  TTTTTGTTTCATTCAGTGGTACTTCAGCAGAGTTAATATGCTTTTCTGATGAATTACACA
  ACAGTTTGTTAATTCTTCATTCTTGTAGTATTTCATCACAAGAAACCTACTCTTCTGTCA
  TCTTGAAGTAAATAGAAGATCAAGCCTTCAAATCTCTTAATTTTTTCGGTATTTATTAAA
  TCTGTGAGTGGTTTAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002873
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002873.1, NP_002864.1
• RefSeq Size: 3164 bp
• RefSeq ORF: 2013 bp
• Locus ID: 5884
• Cytogenetics: 5q13.2
• Domains: Rad17
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (8) has an alternate 5' terminal sequence and lacks an internal exon in the 5' UTR, when compared to variant 1. The encoded protein (isoform 1) is identical to that encoded by variants 1, 5, 6, 7 and 9.