DAK (TKFC) (NM_015533) Human Untagged Clone

CAT#: SC324128

DAK (untagged)-Human dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TKFC
• Synonyms: DAK; NET45
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015533.3 AGCAGCTCAGTGCAACGGTGTGAACTCAGCCTGTTTCAGAGCCTCCACACCATGACCTCC
  AAGAAGCTGGTGAACTCGGTGGCTGGCTGTGCTGATGACGCTCTTGCTGGCCTGGTGGCC
  TGCAACCCCAACCTGCAGCTCCTGCAGGGCCACCGCGTGGCCCTCCGTTCTGACCTGGAC
  AGCCTCAAGGGCCGGGTGGCACTGCTGTCGGGTGGGGGCTCTGGCCATGAGCCTGCCCAT
  GCTGGTTTCATAGGGAAGGGGATGCTGACTGGGGTCATCGCGGGAGCTGTGTTCACCTCC
  CCGGCAGTGGGCAGCATCCTGGCAGCCATCAGGGCCGTGGCCCAGGCCGGCACAGTGGGG
  ACGCTCCTTATCGTGAAGAACTACACTGGGGATCGGCTCAACTTCGGCCTGGCCCGGGAG
  CAGGCCCGGGCTGAAGGCATCCCGGTGGAGATGGTGGTGATTGGGGACGACAGCGCCTTC
  ACTGTCCTGAAGAAGGCAGGCCGGCGGGGGCTGTGCGGCACGGTGCTTATACACAAGGTG
  GCAGGTGCTCTGGCTGAGGCTGGTGTGGGGCTGGAGGAGATCGCAAAGCAGGTGAACGTG
  GTCGCCAAGGCCATGGGTACCCTGGGGGTGAGCTTATCCTCCTGCAGCGTCCCTGGTTCC
  AAACCCACCTTCGAGCTCTCAGCCGACGAGGTGGAGCTGGGCCTGGGGATCCACGGGGAA
  GCTGGTGTGCGCCGGATAAAGATGGCAACCGCCGATGAGATTGTGAAACTCATGCTCGAC
  CACATGACAAACACCACCAACGCGTCCCATGTGCCTGTGCAGCCCGGCTCCTCAGTTGTG
  ATGATGGTCAACAACCTGGGTGGCCTGTCATTCCTGGAACTGGGCATCATAGCCGACGCT
  ACCGTCCGCTCCCTGGAGGGCCGCGGGGTGAAGATTGCCCGTGCCCTGGTGGGCACCTTC
  ATGTCAGCACTGGAGATGCCTGGCATTTCTCTCACCCTCCTGCTGGTGGATGAGCCTCTC
  CTGAAACTGATAGATGCTGAAACCACTGCAGCAGCCTGGCCTAACGTGGCTGCAGTCTCC
  ATTACTGGGCGGAAGCGGAGCCGGGTAGCCCCTGCCGAGCCCCAGGAGGCCCCTGATTCC
  ACTGCTGCAGGAGGCTCAGCCTCGAAGCGGATGGCGCTGGTGCTGGAACGGGTGTGCAGC
  ACTCTCCTGGGCCTGGAGGAACACCTGAATGCCCTGGACCGGGCTGCTGGTGACGGCGAC
  TGTGGCACCACCCACAGCCGTGCGGCCAGAGCAATCCAGGAGTGGCTGAAGGAGGGCCCA
  CCCCCTGCCAGCCCTGCCCAGCTGCTCTCCAAGTTGTCTGTCCTGCTCCTGGAGAAGATG
  GGAGGCTCATCTGGGGCGCTCTATGGCCTGTTCCTGACTGCGGCTGCACAGCCCCTGAAG
  GCCAAGACCAGCCTCCCAGCCTGGTCTGCTGCCATGGATGCCGGCCTGGAAGCCATGCAG
  AAGTATGGCAAGGCTGCTCCAGGGGACAGGACTATGCTGGATTCTCTGTGGGCAGCGGGG
  CAGGAGCTCCAAGCCTGGAAGAGCCCAGGAGCTGATCTGTTACAAGTCCTGACCAAAGCA
  GTCAAGAGTGCCGAAGCTGCAGCCGAGGCCACCAAGAATATGGAAGCTGGAGCCGGAAGA
  GCCAGTTATATCAGCTCAGCACGGCTGGAGCAGCCAGACCCCGGGGCGGTGGCAGCTGCT
  GCCATCCTCCGGGCCATCTTGGAGGTCTTGCAGAGCTAGGGTGTGTGACTGCCTCCCTTG
  GCCTCAGCTCCTCTCACTGCTGTGCTGAGGTGGCCTTTGTCACTTCCTTCTGCCTTCCAA
  CCCTCACCTTCCCCCGGCCTGGCCCCATTGGCCCACCCTCTAAGTTGAGCAGGAAATCCT
  CCACCAAGCTTCCAGAACTACAGACAGCACCCAGAGTGAGCTGGAGTGGGTCCCCATGCC
  TCTCCAGCATGCCCTTTCCCTTTGCAGGAGGGTGGAGTCCCTGGGTCATGCCCTCCCCTG
  CCAGCTCTGGGCTTCAGAGATAAGGCATTTTCCTTGTGCAGCCTTTACCTGGCAATCCTA
  ATTTGGTTTTAAGACTCCCTGTGAAATGCTTTCCGCACCTTAACCCCAGTGAGCGTGAAA
  AAGAAAGTTAATAAACTATAATACATGGAAGCAAGAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015533.3 unedited
  GACGCTTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGTCCG
  GAATTCCCGGGATATCGTCGACCCACGCGTCCGAGCAGCTCAGTGCAACGGTGTGAACTC
  AGCCTGTTTCAGAGCCTCCACACCATGACCTCCAAGAAGCTGGTGAACTCGGTGGCTGGC
  TGTGCTGATGACGCTCTTGCTGGCCTGGTGGCCTGCAACCCCAACCTGCAGCTCCTGCAG
  GGCCACCGCGTGGCCCTCCGTTCTGACCTGGACAGCCTCAAGGGCCGGGTGGCACTGCTG
  TCGGGTGGGGGCTCTGGCCATGAGCCTGCCCATGCTGGTTTCATAGGTGGTTGGTATGCT
  GACTGGGGTCATCGCGGGAGCTGTGTTCACCTCCCCGGCAATGTTTTTTTTTGTGGGGGG
  CCATCACGGCCGTGGCCCAGGCCGGCAAGTTTCGACGCTACTTATAGTGCACAAATACAC
  TGCAATCGGATAAAAAAAAAAAAAACCATAAAAAAATACACCGCTGACTGATTTTAGATG
  AGGAATTGCAG
• Restriction Sites: ECoRI-NOT
• ACCN: NM_015533
• ORF Size: 1728 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_015533.3, NP_056348.2
• RefSeq Size: 4248
• RefSeq ORF: 1728
• Locus ID: 26007
• Domains: Dak1, Dak2
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways, RIG-I-like receptor signaling pathway
• Gene Summary: This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2017]
  Transcript Variant: This variant (2), as well as variant 3, encodes isoform b.