DAK (TKFC) (NM_015533) Human Untagged Clone

CAT#: SC112650

DAK (untagged)-Human dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TKFC
• Synonyms: DAK; NET45
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112650 sequence for NM_015533 edited (data generated by NextGen Sequencing)
  ATGACCTCCAAGAAGCTGGTGAACTCGGTGGCTGGCTGTGCTGATGACGCTCTTGCTGGC
  CTGGTGGCCTGCAACCCCAACCTGCAGCTCCTGCAGGGCCACCGCGTGGCCCTCCGTTCT
  GACCTGGACAGCCTCAAGGGCCGGGTGGCACTGCTGTCGGGTGGGGGCTCTGGCCATGAG
  CCTGCCCATGCTGGTTTCATAGGGAAGGGGATGCTGACTGGGGTCATCGCGGGAGCTGTG
  TTCACCTCCCCGGCAGTGGGCAGCATCCTGGCAGCCATCAGGGCCGTGGCCCAGGCCGGC
  ACAGTGGGGACGCTCCTTATCGTGAAGAACTACACTGGGGATCGGCTCAACTTCGGCCTG
  GCCCGGGAGCAGGCCCGGGCTGAAGGCATCCCGGTGGAGATGGTGGTGATTGGGGACGAC
  AGCGCCTTCACTGTCCTGAAGAAGGCAGGCCGGCGGGGGCTGTGCGGCACCGTGCTTATA
  CACAAGGTGGCAGGTGCTCTGGCTGAGGCTGGTGTGGGGCTGGAGGAGATCGCAAAGCAG
  GTGAACGTGGTCACCAAGGCCATGGGTACCCTGGGGGTGAGCTTATCCTCCTGCAGCGTC
  CCTGGTTCCAAACCCACCTTCGAGCTCTCAGCCGACGAGGTGGAGCTGGGCCTGGGGATC
  CACGGGGAAGCTGGTGTGCGCCGGATAAAGATGGCAACCGCCGATGAGATTGTGAAACTC
  ATGCTCGACCACATGACAAACACCACCAACGCGTCCCATGTGCCTGTGCAGCCCGGCTCC
  TCAGTTGTGATGATGGTCAACAACCTGGGTGGCCTGTCATTCCTGGAACTGGGCATCATA
  GCCGACGCTACCGTCCGCTCCCTGGAGGGCCGCGGGGTGAAGATTGCCCGTGCCCTGGTG
  GGCACCTTCATGTCAGCACTGGAGATGCCTGGCATTTCTCTCACCCTCCTGCTGGTGGAT
  GAGCCTCTCCTGAAACTGATAGATGCTGAAACCACTGCAGCAGCCTGGCCTAACGTGGCT
  GCAGTCTCCATTACTGGGCGGAAGCGGAGCCGGGTAGCCCCTGCCGAGCCCCAGGAGGCC
  CCTGATTCCACTGCTGCAGGAGGCTCAGCCTCGAAGCGGATGGCGCTGGTGCTGGAACGG
  GTGTGCAGCACTCTCCTGGGCCTGGAGGAACACCTGAATGCCCTGGACCGGGCTGCTGGT
  GACGGCGACTGTGGCACCACCCACAGCCGTGCGGCCAGAGCAATCCAGGAGTGGCTGAAG
  GAGGGCCCACCCCCTGCCAGCCCTGCCCAGCTGCTCTCCAAGTTGTCTGTTCTGCTCCTG
  GAGAAGATGGGAGGCTCATCTGGGGCGCTCTATGGCCTGTTCCTGACTGCGGCTGCACAG
  CCCCTGAAGGCCAAGACCAGCCTCCCAGCCTGGTCTGCTGCCATGGATGCCGGCCTGGAA
  GCCATGCAGAAGTATGGCAAGGCTGCTCCAGGGGACAGGACTATGCTGGATTCTCTGTGG
  GCAGCGGGGCAGGAGCTCCAAGCCTGGAAGAGCCCAGGAGCTGATCTGTTACAAGTCCTG
  ACCAAAGCAGTCAAGAGTGCCGAAGCTGCAGCCGAGGCCACCAAGAATATGGAAGCTGGA
  GCCGGAAGAGCCAGTTATATCAGCTCAGCACGGCTGGAGCAGCCAGACCCCGGGGCGGTG
  GCAGCTGCTGCCATCCTCCGGGCCATCTTGGAGGTCTTGCAGAGCTAG
  
  Clone variation with respect to NM_015533.3
  471 g=>c;553 g=>a;1311 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015533 unedited
  NTGTTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCGCACGCT
  TCGGGGTCTCCGGGNAGTCGCGGCGCCTTCGGATGTGGCGGATGCGGCCGTGAGCCGGCG
  GGGGAGGTGCTGCTGCTGCCTCCACTGTACTCAGACCCAGGTAGCACAGGATTGTCCATC
  CTCCAGCAGCTCAGTGCAACGGTGTGAACTCAGCCTGTTTCAGAGCCTCCACACCATGAC
  CTCCAAGAAGCTGGTGAACTCGGTGGCTGGCTGTGCTGATGACGCTCTTGCTGGCCTGGT
  GGCCTGCAACCCCAACCTGCAGCTCCTGCAGGGCCACCGCGTGGCCCTCCGTTCTGACCT
  GGACAGCCTCAAGGGCCGGGTGGCACTGCTGTCGGGTGGGGGCTCTGGCCATGAGCCTGC
  CCATGCTGGTTTCATAGGGAAGGGGATGCTGACTGGGGTCATCGCGGGAGCTGTGTTCAC
  CTCCCCGGCAGTGGGCAGCATCCTGGCAGCCATCAGGGCCGTGGCCCAGGCCGGCACAGT
  GGGGACGCTCCTTATCGTGAAGAACTACACTGGGGATCGGCTCAACTTCGGCCTGGCCCG
  GGAGCAGGCCCGGGCTGAAGGCATCCCGGTGGAGATGGTGGTGATTGGGGACGACAGCGC
  CTTCACTGTCCTGAAGAAGCAGGCCGGCGGNGGCTGTGCGGCACCGTGCTTATACACAAG
  GTGGCAGGTGCTCTGGCTGANNGCTGTGTGGNGCTGGNAGAGATCGCANAGCAGGTGAAC
  GTGGTCACCAAGCCATGGGTACCCTGNGGGTGAGCTNATTCTCCTGCAGCGTCCCTGGNT
  CCAAACCCACTTCGAGCTCTCAGCCGACGAGGGGGAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015533 unedited
  CCGCGGCCGCAATTTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTT
  CCATGTATTATAGTTTATTAACTTTCTTTTTCACGCTCACTGGGGTTAAGGGGCGGAAAG
  CATTTCACAGGGAGTCTTAAAACCAAATTAGGATTGCCAGGTAAAGGCTGCACAAGGAAA
  ATGCCTTATCTCTGAAGCCCAAAGCTGGCAGGGGAGGGCATGACCCAGGGACTCCACCCT
  CCTGCAAAGGGAAAGGGCATGCTGGAGAGGCATGGGGACCCACTCCAGCTCACTCTGGGT
  GCTGTCTGTAGTTCTGGAAGCTTGGTGGAGGATTTCCTGCTCAACTTAAAGGGTGGGCCA
  ATGGGGCCAGGCCGGGGGAAGGTGAGGGTTGGAAGGCAAAAGGAAGTGACAAAGGCCACC
  TCAACACAGCAGTGAGAGGAGCTGAGGCCAAGGGAGGCAGTCACACACCCTAGCTCTGCA
  AGACCTCCAAGATGGCCCGGAGGATGGCAGCAGCTGCCACCGCCCCGGGGTCTGGCTGCT
  CCAGCCGTGCTGAGCTGATATAACTGGCTCTTCCGGCTCCAGCTTCCATATTCTTGGTGG
  CCTCGGCTGCAGCTTCGGCACTCTTGACTGCTTTGGTCAGGACCTTGAACAGATCAGCTT
  CTGGGCTCTTCCAGCTTGGAGCTCCTGCCCCGCTGCCCCCAGAGAATCAGCATAATCCTG
  TCCCCTGGAGCAGCCCTGGCATATTTCTGCATGGGTTCCAAGCCCGCATTCCAGGCNAGC
  AGACCCGGCTGGGGAGGCTGGTCTTGGCCTTCAAGGGCTGTGCACCCGCATTCAGGAACA
  CGCCTAAAACGCCCCCATGAACCCTCCATTTTTTCCGGAGCAGAAAAAACCTTGGAACCA
  CCTGCCAAGCCTGGAAGGGGGGGCCCCTCTTCAACACTCTGGG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_015533
• ORF Size: 1728 bp
• Insert Size: 2500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_015533.2, NP_056348.2
• RefSeq Size: 2210
• RefSeq ORF: 1728
• Locus ID: 26007
• Domains: Dak1, Dak2
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways, RIG-I-like receptor signaling pathway
• Gene Summary: This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2017]
  Transcript Variant: This variant (2), as well as variant 3, encodes isoform b.