14 3 3 gamma (YWHAG) (NM_012479) Human Untagged Clone

CAT#: SC324155

YWHAG (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: YWHAG
• Synonyms: 14-3-3GAMMA; EIEE56; PPP1R170
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012479.2 CAGCAGCCGCAGCCGCCTCGCGCCCGGTCCCGCGGTCGCAGCTCCAGCCGCCTCCTCCGC
  GCAGCCGCCGCCTCAGCTGCTCGCTCTGTGGGTCGGTCCTCTCCGGCACTTGGGCTCCAG
  TCGCGCCCTCCAAGCCCTTCAGGCCGCCCCAGTGTCCTCCTCCTTCTCCGGCCAGACCCA
  GCCCCGCGAAGATGGTGGACCGCGAGCAACTGGTGCAGAAAGCCCGGCTGGCCGAGCAGG
  CGGAGCGCTACGACGACATGGCCGCGGCCATGAAGAACGTGACAGAGCTGAATGAGCCAC
  TGTCGAATGAGGAACGAAACCTTCTGTCTGTGGCCTACAAGAACGTTGTGGGGGCACGCC
  GCTCTTCCTGGAGGGTCATCAGTAGCATTGAGCAGAAGACATCTGCAGACGGCAATGAGA
  AGAAGATTGAGATGGTCCGTGCGTACCGGGAGAAGATAGAGAAGGAGTTGGAGGCTGTGT
  GCCAGGATGTGCTGAGCCTGCTGGATAACTACCTGATCAAGAATTGCAGCGAGACCCAGT
  ACGAGAGCAAAGTGTTCTACCTGAAGATGAAAGGGGACTACTACCGCTACCTGGCTGAAG
  TGGCCACCGGAGAGAAAAGGGCGACGGTGGTGGAGTCCTCTGAGAAGGCCTACAGCGAAG
  CCCACGAGATCAGCAAAGAGCACATGCAGCCCACCCACCCCATCCGATTAGGCCTGGCTC
  TTAACTACTCCGTCTTCTACTATGAGATCCAGAACGCCCCAGAGCAAGCGTGCCACTTGG
  CCAAGACCGCGTTCGACGACGCCATCGCCGAGCTTGACACCCTCAACGAGGACTCCTACA
  AGGACTCCACGCTCATCATGCAGCTCCTCCGCGACAACCTCACGCTCTGGACGAGCGACC
  AGCAGGACGACGATGGCGGCGAAGGCAACAATTAAGGCCCCAGGGGAACTGGCAGCGCAC
  GCGGATGCTACTACTGCAGTCTTTATTTTTTTCCCATGAGTTGGGGGTCGGGTGGGGGAG
  GGAAAGGGAGGGATGACCTTCCCAGGGAGAAACCCACGACCTGTCCTGTCTTTGATCGCC
  TCTTTGACATTTTTGCCAAAATACCACTAGTGGAAAGTCAGGCTAGCTGTGCTGGTATTG
  GAATAGCAGCCTCACACTGGCGTCTGGACTGTTCTGTAGATTCATGCAAGTGGAGCTGTC
  TGTCTCTAATTTAACTTATTGCTAGATAATAGGGTTTTCAGATGAAAAGAAAACTTAAAG
  AGGAATGGCCCTCATTCAGTAAGTTCTGTGGTTCCAGTAAGGATTTTTATGTACATACGC
  TCTCGTCTCTCGTTTTGGGTACTTTCTATCTCATCTGTCTCGGCTCTGCATGTTTTCCAG
  GGTGTAGCCTACAGACATGGAACAGTGTAAATCCCAGACTGACAGACTTAGAACCTGAGG
  TCTCATTCATCCTTATGGTTTAGGCCTTGCCAGTTTTCCGAAGTCTCTGATTAGTTGACA
  GTATTAACACTAAATTGCAGTTTACAGTATTTCTACATTACAGCCATATGTAACATCAAG
  CCATCGATTGTGTACTTTTCCTTTGCTAGTTGTTTGGGCTTTAACATCCTTATTCAGCCT
  TATCCAGGTTGGTTTTGCTGTTGATCGGTCTCCTAGGCTAAATGAGAATGAAAGCGACTT
  CAGGTTTTTGGTTCATAGGTGCTCGGCAGGTGGCTGTGGGATTTTTTTTTTTTTTGGTCC
  TTCTTTCCTCTTAACGTAAATCCACCACCAAAATTATTAATCCTCTTGAGAGAAACGTGA
  AACGCCACAAAAATAGAGAAAATTCAGGTCTGTATGTCATGGATCGTGTTGGTATTTTCA
  GAGAACATCCCGCTTCTGAAGCTGCTGCAGCTCCCTCCTCAGGGATCACACTGCCGTCAC
  CCACTCTGCACTGGGGCGTTTCCTACTGCGCCTCGTGCTGGCGGACGCAGCTGGGTGCAG
  AAGCTGTGGGGTCGGAGAGGCGTTTGGAGAAGGTCTGTGGTGCAGTGTGTGAAAATTCAG
  GTGCTAGAAGCCTACTGGTAGAAAAACCCAAAAGGAAGAGCTATATCCTTAACCATTCTG
  TCCAATTTCGGGAGCCTTGTCAGTGTGTCAGTTTTTCCTCCCCGAAGACACTCCTTCCCC
  AAGTAATTGTAGGAAGATAAAAAAACTGTTACCAGATAACAAACACTGAACTCCTATTTG
  ACCAGAACTTTTTCCTCTCGAGATAGTTTTTTCTTTTTAATGAAAAAAGCATAGGAATTG
  GAGATTGGCTTGTCTCACGCAGCCAGTGCACATTTGGAATTGACGGAAACAACGTTGCTA
  TTTCCACCCATTTGTTTTCGGCAGCCTTAAGGCCCTCATTCTCATTTCGGGTGAATCTGT
  CTATCTGTGAACGTGGCCCGCATGTGCATTCTTTTTTTATATATATAAAGTCAGTGACGA
  GGAACTCCCGAGACGTGTAATGACACCACACTTGTTTTCTTTGTTTCTTTGTTTTATTTA
  GGCAAGAAGAGGTGTGAGTAATTGAGGAAAAACTGACAGATGCTTTTGCTAATACCAAAA
  TTGAGCTTACAATTAGGAACTGAGTATGTGTAACAGGATACAGGTGACAGTGAAGATAGA
  AGAACCACGATGACCACAGACTCAATGTGCTCTGTAACATCGCACAGTTTACCCAGCATG
  ACTTTCCTTAGGAGGCCCCCTCCTCACGCTAGAGTAAAAGTCCCAGTTAAGTGAAGCCTA
  CCAGAAGAACTAGTAGAAGAAGCTTTGCCGCTTTTGTGCCTCTCACAGGCGCCTAAAGTC
  ATTGCCATGGGAGGAAGACGATTTGGGGGGGGGGCAGGGTAGGTGGGGCTTTCCCTAATT
  TATCTTCATGTCCAGTGAGCAGTGTTGCGTTTTTCCTTGTAGCATTTGGAAATGATTTAC
  TGGAATTACAAAACCTATTTTTCCTTTAAATTTCAGCTTTGGCTCTGGCTGCTTTTTAGA
  ATAATGCAAGATAAAAATCACACCTGAGGGCTGAAAACGGAGAGGGAATGGGAGACTTGA
  TATTTAAGCAGCTTGAATGGTTTTTCTTTTCTTTATTTTTAAAGAAATGCACTTGCCTAT
  GATACTGTCTCTCCAGTGAAATGATTACTCCTCCATTACTCTATTGATACAATATTGTGC
  ATGCTAGTGTTGTATTTCTATACAGTAGCTTGAAATTGATTAACTTATACTGTAGGTGTT
  ATGTATTCCTATGACAAAAAAAATTAAGTCTTCAAATTTTTTAAAGGTTTTTTTTTTTAA
  TTTAATTTTTCCTTTTGGGGGTAAAGTTTGCTCTACCAAATAGTGATTGTAACAAATTGA
  TCTGTTTTGGATGTTGCTATAGTGACATGCAGTTATATATTTTGTTTTTAAAAGGGGGGG
  AGCAAAAGAAACACCAGTGTTAGCTTAATCTTAATGTCTGGTGTTTGTCATGGTGAAATT
  ATAACTATTACAGTGTTGGAGAACAACAAATATGTTCTCTGAATGAGCCTTTGTGCTTTT
  TGTCATGTTATGCAGTGAACTATTTTTAAGGTCTAATCAGTGATTATTTTTCCAGCTCCG
  TGTTTCTCTAAGGAATTATTTCACACACGGACCATCTTTAGCAGTTTCCTCAGTGATGGA
  ATATCATGAATGTGAGTCATTATGTAGCTGTCGTACATTGAGCAAATAAACTTACAGATC
  TGAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_012479
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012479.2, NP_036611.2
• RefSeq Size: 3747 bp
• RefSeq ORF: 744 bp
• Locus ID: 7532
• Cytogenetics: 7q11.23
• Domains: 14-3-3
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis
• Gene Summary: 'This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]'