Cytokeratin 6B (KRT6B) (NM_005555) Human Untagged Clone

CAT#: SC324177

KRT6B (untagged)-Human keratin 6B (KRT6B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT6B
• Synonyms: CK-6B; CK6B; K6B; KRTL1; PC2; PC4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005555.3 CGCCTCCAGCCTCTCACACTCTCCTAAGCCCTCTCATCTCCTGGAACCATGGCCAGCACA
  TCCACCACCATCAGGAGCCACAGCAGCAGCCGCCGGGGTTTCAGTGCCAACTCAGCCAGG
  CTCCCTGGGGTCAGCCGCTCTGGCTTCAGCAGCATCTCCGTGTCCCGCTCCAGGGGCAGT
  GGTGGCCTGGGTGGCGCATGTGGAGGAGCTGGCTTTGGCAGCCGCAGTCTGTATGGCCTG
  GGGGGCTCCAAGAGGATCTCCATTGGAGGGGGCAGCTGTGCCATCAGTGGCGGCTATGGC
  AGCAGAGCCGGAGGCAGCTATGGCTTTGGTGGCGCCGGGAGTGGATTTGGTTTCGGTGGT
  GGAGCCGGCATTGGCTTTGGTCTGGGTGGTGGAGCCGGCCTTGCTGGTGGCTTTGGGGGC
  CCTGGCTTCCCTGTGTGCCCCCCTGGAGGCATCCAAGAGGTCACTGTCAACCAGAGTCTC
  CTGACTCCCCTCAACCTGCAAATTGACCCCGCCATCCAGCGGGTGCGGGCCGAGGAGCGT
  GAGCAGATCAAGACCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCGGTTCCTA
  GAGCAGCAGAACAAGGTTCTGGACACCAAGTGGACCCTGCTGCAGGAGCAGGGCACCAAG
  ACTGTGAGGCAGAACCTGGAGCCGTTGTTCGAGCAGTACATCAACAACCTCAGGAGGCAG
  CTGGACAACATCGTGGGGGAACGGGGTCGTCTGGACTCGGAGCTGAGAAACATGCAGGAC
  CTGGTGGAGGACCTCAAGAACAAATATGAGGATGAAATCAACAAGCGCACAGCAGCAGAG
  AATGAATTTGTGACTCTGAAGAAGGATGTGGATGCTGCCTACATGAACAAGGTTGAACTG
  CAAGCCAAGGCAGACACTCTTACAGATGAGATCAACTTCCTGAGAGCCTTGTATGATGCA
  GAGCTGTCCCAGATGCAGACCCACATCTCAGACACATCCGTGGTGCTATCCATGGACAAC
  AACCGCAACCTGGACCTGGACAGCATCATCGCTGAGGTCAAGGCCCAATATGAGGAGATT
  GCTCAGAGGAGCAGGGCTGAGGCTGAGTCCTGGTACCAGACAAAGTACGAGGAGCTGCAG
  ATCACAGCAGGCAGACATGGGGACGACCTGCGCAACACCAAGCAGGAGATTGCTGAGATC
  AACCGCATGATCCAGAGGCTGAGATCTGAGATCGACCACGTCAAGAAGCAGTGTGCCAAC
  CTACAGGCCGCCATTGCTGATGCTGAGCAGCGTGGGGAGATGGCCCTCAAGGATGCTAAG
  AACAAGCTGGAAGGGCTGGAGGATGCCCTGCAGAAGGCCAAGCAGGACCTGGCCCGGCTG
  CTGAAGGAGTACCAGGAGCTGATGAACGTCAAGCTGGCCCTGGATGTGGAGATCGCCACC
  TACCGCAAGCTGCTGGAGGGCGAGGAGTGCAGGCTGAATGGCGAAGGCGTTGGACAAGTC
  AACATCTCTGTAGTGCAGTCCACCGTCTCCAGTGGCTATGGCGGTGCCAGCGGTGTCGGC
  AGTGGCTTAGGCCTGGGTGGAGGAAGCAGCTACTCCTATGGCAGTGGTCTTGGCGTTGGA
  GGCGGCTTTAGTTCCAGCAGCGGCAGAGCCACTGGGGGTGGCCTCAGCTCTGTTGGAGGC
  GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAGCTACAAGCAC
  TGAAGTGCTGCCGCCAGCTCTCAGTCCCACAGCTCTCAGGCCCCTCTCTGGCAGCAGAGC
  CCTCTCCTCAGGTTGCTTGTCCTCCCCTGGCCTCCAGTCTCCCCTGCCCTCCCGGGTAGA
  GCTGGGATGCCCTCACTTTTCTTCTCATCAATACCTGTTCCACTGAGCTCCTGTTGCTTA
  CCATCAAGTCAACAGTTATCAGCACTCAACATGCGAATGTCCTTTTTAGTTCCCGTATTA
  TTACAGGTATCTGAGTCTGCCATAATTCTGAGAAGAAAATGACCTATATCCCCATAAGAA
  CTGAAACTCAGTCTAGGTCCAGCTGCAGATGAGGAGTCCTCTCTTTAATTGCTAACCATC
  CTGCCCATTATAGCTACACTCAGGAGTTCTCATCTGACAAGTCAGTTGTCCTGATCTTCT
  CTTGCAGTGTCCCTGAATGGCAAGTGATGTACCTTCTGATGCAGTCTGCATTCCTGCACT
  GCTTTCTCTGCTCTCTTTGCCTTCTTTTGTTCTGTTGAATAAAGCATATTGAGAATGTGA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005555
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005555.3, NP_005546.2
• RefSeq Size: 2331 bp
• RefSeq ORF: 1695 bp
• Locus ID: 3854
• Cytogenetics: 12q13.13
• Gene Summary: 'The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]'