TDP1 (NM_001008744) Human Untagged Clone

CAT#: SC324260

TDP1 (untagged)-Human tyrosyl-DNA phosphodiesterase 1 (TDP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TDP1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001008744.1 GTTGGTTCTGTGCGCCTCAGAGTTGGAGCACACAGCTGTATTAAAAAGGCAAATCGAAGG
  CCGGGCGCGGTGACTCACGCCTGTCATCCTAGCACTTTGGGAGGCCGAGGCGGCTGAATC
  ACTTGAGGTTAGGAGTTTGAGATCAGCCCGGGCAACATGGTGAAACCCCGTCTCTACAAA
  AATAGAAAAATTAGCCGAGCGTGATGGTGGATGCCTGTAATCCTAGCTCCTCGGGAGGCT
  AAGGAGTATAATGTCTCAGGAAGGCGATTATGGGAGGTGGACCATATCTAGTAGTGATGA
  AAGTGAGGAAGAAAAGCCAAAACCAGACAAGCCATCTACCTCTTCTCTTCTCTGTGCCAG
  GCAAGGAGCAGCAAATGAGCCCAGGTACACCTGTTCCGAGGCCCAGAAAGCTGCACACAA
  GAGGAAAATATCACCTGTGAAATTCAGCAATACAGATTCAGTTTTACCTCCCAAAAGGCA
  GAAAAGCGGTTCCCAGGAGGACCTCGGCTGGTGTCTGTCCAGCAGTGATGATGAGCTGCA
  ACCAGAAATGCCGCAGAAGCAGGCTGAGAAAGTGGTGATCAAAAAGGAGAAAGACATCTC
  TGCTCCCAATGACGGCACTGCCCAAAGAACTGAAAATCATGGCGCTCCCGCCTGCCACAG
  GCTCAAAGAGGAGGAAGACGAGTATGAGACATCAGGGGAGGGCCAGGACATTTGGGACAT
  GCTGGATAAAGGGAACCCCTTCCAGTTTTACCTCACTAGAGTCTCTGGAGTTAAGCCAAA
  GTATAACTCTGGAGCCCTCCACATCAAGGATATTTTATCTCCTTTATTTGGGACGCTTGT
  TTCTTCAGCTCAGTTTAACTACTGCTTTGACGTGGACTGGCTCGTAAAACAGTATCCACC
  AGAGTTCAGGAAGAAGCCAATCCTGCTTGTGCATGGTGATAAGCGAGAGGCTAAGGCTCA
  CCTCCATGCCCAGGCCAAGCCTTACGAGAACATCTCTCTCTGCCAGGCAAAGTTGGATAT
  TGCGTTTGGAACACACCACACGAAAATGATGCTGCTGCTCTATGAAGAAGGCCTCCGGGT
  TGTCATACACACCTCCAACCTCATCCATGCTGACTGGCACCAGAAAACTCAAGGAATATG
  GTTGAGCCCCTTATACCCACGAATTGCTGATGGAACCCACAAATCTGGAGAGTCGCCAAC
  ACATTTTAAAGCTGATCTCATCAGTTACTTGATGGCTTATAATGCCCCTTCTCTCAAGGA
  GTGGATAGATGTCATTCACAAGCACGATCTCTCTGAAACAAATGTTTATCTTATTGGTTC
  AACCCCAGGACGCTTTCAAGGAAGTCAAAAAGATAATTGGGGACATTTTAGACTTAAGAA
  GCTTCTGAAAGACCATGCCTCATCCATGCCTAACGCAGAGTCCTGGCCTGTCGTAGGTCA
  GTTTTCAAGCGTTGGCTCCTTGGGAGCCGATGAATCAAAGTGGTTATGTTCTGAGTTTAA
  AGAGAGCATGCTGACACTGGGGAAGGAAAGCAAGACTCCAGGAAAAAGCTCTGTTCCTCT
  TTACTTGATCTATCCTTCTGTGGAAAATGTGCGGACCAGTTTAGAAGGATATCCTGCTGG
  GGGCTCTCTTCCCTATAGCATCCAGACAGCTGAAAAACAGAATTGGCTGCATTCCTATTT
  TCACAAATGGTCAGCTGAGACTTCTGGCCGCAGCAATGCCATGCCACATATTAAGACATA
  TATGAGGCCTTCTCCAGACTTCAGTAAAATTGCTTGGTTCCTTGTCACAAGCGCAAATCT
  GTCCAAGGCTGCCTGGGGAGCATTGGAGAAGAATGGCACCCAGCTGATGATCCGCTCCTA
  CGAGCTCGGGGTCCTTTTCCTCCCTTCAGCATTTGGTCTAGACAGTTTCAAAGTGAAACA
  GAAGTTCTTCGCTGGCAGCCAGGAGCCAATGGCCACCTTTCCTGTGCCATATGATTTGCC
  TCCAGAACTGTATGGAAGTAAAGATCGGCCATGGATATGGAACATTCCTTATGTCAAAGC
  ACCGGATACGCATGGGAACATGTGGGTGCCCTCCTGAGAATCTTGAGGCACTGTGAAATT
  TAAGTGTAAGACATTGAGCCACAAACATGGAATCTCTTCTTTGTACTGGATGTCCACTTC
  CCTTAAAGTCTTATTTGCACCCTTACAAAATCTTTCCAAAGGTCACTCTTATGAATGGAT
  GTTGGTTATACTTTTAATGGACATTAACATTCCTAATAAAGTATTAGTTTCTTAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAA
• Restriction Sites: Please inquire
• ACCN: NM_001008744
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008744.1, NP_001008744.1
• RefSeq Size: 3540 bp
• RefSeq ORF: 1827 bp
• Locus ID: 55775
• Cytogenetics: 14q32.11
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). [provided by RefSeq, Aug 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (a).