TPP1 (NM_000391) Human Untagged Clone

CAT#: SC324263

TPP1 (untagged)-Human tripeptidyl peptidase I (TPP1)


  "NM_000391" in other vectors (7)

Reconstitution Protocol

SC324263 is the updated version of SC119896.

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TPP1
Synonyms CLN2; GIG1; LPIC; SCAR7; TPP-1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_000391.3 CGGAAGGGCAGAATGGGACTCCAAGCCTGCCTCCTAGGGCTCTTTGCCCTCATCCTCTCT
GGCAAATGCAGTTACAGCCCGGAGCCCGACCAGCGGAGGACGCTGCCCCCAGGCTGGGTG
TCCCTGGGCCGTGCGGACCCTGAGGAAGAGCTGAGTCTCACCTTTGCCCTGAGACAGCAG
AATGTGGAAAGACTCTCGGAGCTGGTGCAGGCTGTGTCGGATCCCAGCTCTCCTCAATAC
GGAAAATACCTGACCCTAGAGAATGTGGCTGATCTGGTGAGGCCATCCCCACTGACCCTC
CACACGGTGCAAAAATGGCTCTTGGCAGCCGGAGCCCAGAAGTGCCATTCTGTGATCACA
CAGGACTTTCTGACTTGCTGGCTGAGCATCCGACAAGCAGAGCTGCTGCTCCCTGGGGCT
GAGTTTCATCACTATGTGGGAGGACCTACGGAAACCCATGTTGTAAGGTCCCCACATCCC
TACCAGCTTCCACAGGCCTTGGCCCCCCATGTGGACTTTGTGGGGGGACTGCACCGTTTT
CCCCCAACATCATCCCTGAGGCAACGTCCTGAGCCGCAGGTGACAGGGACTGTAGGCCTG
CATCTGGGGGTAACCCCCTCTGTGATCCGTAAGCGATACAACTTGACCTCACAAGACGTG
GGCTCTGGCACCAGCAATAACAGCCAAGCCTGTGCCCAGTTCCTGGAGCAGTATTTCCAT
GACTCAGACCTGGCTCAGTTCATGCGCCTCTTCGGTGGCAACTTTGCACATCAGGCATCA
GTAGCCCGTGTGGTTGGACAACAGGGCCGGGGCCGGGCCGGGATTGAGGCCAGTCTAGAT
GTGCAGTACCTGATGAGTGCTGGTGCCAACATCTCCACCTGGGTCTACAGTAGCCCTGGC
CGGCATGAGGGACAGGAGCCCTTCCTGCAGTGGCTCATGCTGCTCAGTAATGAGTCAGCC
CTGCCACATGTGCATACTGTGAGCTATGGAGATGATGAGGACTCCCTCAGCAGCGCCTAC
ATCCAGCGGGTCAACACTGAGCTCATGAAGGCTGCCGCTCGGGGTCTCACCCTGCTCTTC
GCCTCAGGTGACAGTGGGGCCGGGTGTTGGTCTGTCTCTGGAAGACACGAGTTCCGCCCT
ACCTTCCCTGCCTCCAGCCCCTATGTCACCACAGTGGGAGGCACATCCTTCCAGGAACCT
TTCCTCATCACAAATGAAATTGTTGACTATATCAGTGGTGGTGGCTTCAGCAATGTGTTC
CCACGGCCTTCATACCAGGAGGAAGCTGTAACGAAGTTCCTGAGCTCTAGCCCCCACCTG
CCACCATCCAGTTACTTCAATGCCAGTGGCCGTGCCTACCCAGATGTGGCTGCACTTTCT
GATGGCTACTGGGTGGTCAGCAACAGAGTGCCCATTCCATGGGTGTCCGGAACCTCGGCC
TCTACTCCAGTGTTTGGGGGGATCCTATCCTTGATCAATGAGCACAGGATCCTTAGTGGC
CGCCCCCCTCTTGGCTTTCTCAACCCAAGGCTCTACCAGCAGCATGGGGCAGGACTCTTT
GATGTAACCCGTGGCTGCCATGAGTCCTGTCTGGATGAAGAGGTAGAGGGCCAGGGTTTC
TGCTCTGGTCCTGGCTGGGATCCTGTAACAGGCTGGGGAACACCCAACTTCCCAGCTTTG
CTGAAGACTCTACTCAACCCCTGACCCTTTCCTATCAGGAGAGATGGCTTGTCCCCTGCC
CTGAAGCTGGCAGTTCAGTCCCTTATTCTGCCCTGTTGGAAGCCCTGCTGAACCCTCAAC
TATTGACTGCTGCAGACAGCTTATCTCCCTAACCCTGAAATGCTGTGAGCTTGACTTGAC
TCCCAACCCTACCATGCTCCATCATACTCAGGTCTCCCTACTCCTGCCTTAGATTCCTCA
ATAAGATGCTGTAACTAGCATTTTTTGAATGCCTCTCCCTCCGCATCTCATCTTTCTCTT
TTCAATCAGGCTTTTCCAAAGGGTTGTATACAGACTCTGTGCACTATTTCACTTGATATT
CATTCCCCAATTCACTGCAAGGAGACCTCTACTGTCACCGTTTACTCTTTCCTACCCTGA
CATCCAGAAACAATGGCCTCCAGTGCATACTTCTCAATCTTTGCTTTATGGCCTTTCCAT
CATAGTTGCCCACTCCCTCTCCTTACTTAGCTTCCAGGTCTTAACTTCTCTGACTACTCT
TGTCTTCCTCTCTCATCAATTTCTGCTTCTTCATGGAATGCTGACCTTCATTGCTCCATT
TGTAGATTTTTGCTCTTCTCAGTTTACTCATTGTCCCCTGGAACAAATCACTGACATCTA
CAACCATTACCATCTCACTAAATAAGACTTTCTATCCAATAATGATTGATACCTCAAATG
TAAGATGCGTGATACTCAACATTTCATCGTCCACCTTCCCAACCCCAAACAATTCCATCT
CGTTTCTTCTTGGTAAATGATGCTATGCTTTTTCCAACCAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_000391
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000391.3, NP_000382.3
RefSeq Size 3540 bp
RefSeq ORF 1692 bp
Locus ID 1200
Cytogenetics 11p15.4
Protein Families Protease
Protein Pathways Lysosome
Gene Summary 'This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008]'

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.