TPP1 (NM_000391) Human Untagged Clone

CAT#: SC324263

TPP1 (untagged)-Human tripeptidyl peptidase I (TPP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TPP1
• Synonyms: CLN2; GIG1; LPIC; SCAR7; TPP-1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000391.3 CGGAAGGGCAGAATGGGACTCCAAGCCTGCCTCCTAGGGCTCTTTGCCCTCATCCTCTCT
  GGCAAATGCAGTTACAGCCCGGAGCCCGACCAGCGGAGGACGCTGCCCCCAGGCTGGGTG
  TCCCTGGGCCGTGCGGACCCTGAGGAAGAGCTGAGTCTCACCTTTGCCCTGAGACAGCAG
  AATGTGGAAAGACTCTCGGAGCTGGTGCAGGCTGTGTCGGATCCCAGCTCTCCTCAATAC
  GGAAAATACCTGACCCTAGAGAATGTGGCTGATCTGGTGAGGCCATCCCCACTGACCCTC
  CACACGGTGCAAAAATGGCTCTTGGCAGCCGGAGCCCAGAAGTGCCATTCTGTGATCACA
  CAGGACTTTCTGACTTGCTGGCTGAGCATCCGACAAGCAGAGCTGCTGCTCCCTGGGGCT
  GAGTTTCATCACTATGTGGGAGGACCTACGGAAACCCATGTTGTAAGGTCCCCACATCCC
  TACCAGCTTCCACAGGCCTTGGCCCCCCATGTGGACTTTGTGGGGGGACTGCACCGTTTT
  CCCCCAACATCATCCCTGAGGCAACGTCCTGAGCCGCAGGTGACAGGGACTGTAGGCCTG
  CATCTGGGGGTAACCCCCTCTGTGATCCGTAAGCGATACAACTTGACCTCACAAGACGTG
  GGCTCTGGCACCAGCAATAACAGCCAAGCCTGTGCCCAGTTCCTGGAGCAGTATTTCCAT
  GACTCAGACCTGGCTCAGTTCATGCGCCTCTTCGGTGGCAACTTTGCACATCAGGCATCA
  GTAGCCCGTGTGGTTGGACAACAGGGCCGGGGCCGGGCCGGGATTGAGGCCAGTCTAGAT
  GTGCAGTACCTGATGAGTGCTGGTGCCAACATCTCCACCTGGGTCTACAGTAGCCCTGGC
  CGGCATGAGGGACAGGAGCCCTTCCTGCAGTGGCTCATGCTGCTCAGTAATGAGTCAGCC
  CTGCCACATGTGCATACTGTGAGCTATGGAGATGATGAGGACTCCCTCAGCAGCGCCTAC
  ATCCAGCGGGTCAACACTGAGCTCATGAAGGCTGCCGCTCGGGGTCTCACCCTGCTCTTC
  GCCTCAGGTGACAGTGGGGCCGGGTGTTGGTCTGTCTCTGGAAGACACGAGTTCCGCCCT
  ACCTTCCCTGCCTCCAGCCCCTATGTCACCACAGTGGGAGGCACATCCTTCCAGGAACCT
  TTCCTCATCACAAATGAAATTGTTGACTATATCAGTGGTGGTGGCTTCAGCAATGTGTTC
  CCACGGCCTTCATACCAGGAGGAAGCTGTAACGAAGTTCCTGAGCTCTAGCCCCCACCTG
  CCACCATCCAGTTACTTCAATGCCAGTGGCCGTGCCTACCCAGATGTGGCTGCACTTTCT
  GATGGCTACTGGGTGGTCAGCAACAGAGTGCCCATTCCATGGGTGTCCGGAACCTCGGCC
  TCTACTCCAGTGTTTGGGGGGATCCTATCCTTGATCAATGAGCACAGGATCCTTAGTGGC
  CGCCCCCCTCTTGGCTTTCTCAACCCAAGGCTCTACCAGCAGCATGGGGCAGGACTCTTT
  GATGTAACCCGTGGCTGCCATGAGTCCTGTCTGGATGAAGAGGTAGAGGGCCAGGGTTTC
  TGCTCTGGTCCTGGCTGGGATCCTGTAACAGGCTGGGGAACACCCAACTTCCCAGCTTTG
  CTGAAGACTCTACTCAACCCCTGACCCTTTCCTATCAGGAGAGATGGCTTGTCCCCTGCC
  CTGAAGCTGGCAGTTCAGTCCCTTATTCTGCCCTGTTGGAAGCCCTGCTGAACCCTCAAC
  TATTGACTGCTGCAGACAGCTTATCTCCCTAACCCTGAAATGCTGTGAGCTTGACTTGAC
  TCCCAACCCTACCATGCTCCATCATACTCAGGTCTCCCTACTCCTGCCTTAGATTCCTCA
  ATAAGATGCTGTAACTAGCATTTTTTGAATGCCTCTCCCTCCGCATCTCATCTTTCTCTT
  TTCAATCAGGCTTTTCCAAAGGGTTGTATACAGACTCTGTGCACTATTTCACTTGATATT
  CATTCCCCAATTCACTGCAAGGAGACCTCTACTGTCACCGTTTACTCTTTCCTACCCTGA
  CATCCAGAAACAATGGCCTCCAGTGCATACTTCTCAATCTTTGCTTTATGGCCTTTCCAT
  CATAGTTGCCCACTCCCTCTCCTTACTTAGCTTCCAGGTCTTAACTTCTCTGACTACTCT
  TGTCTTCCTCTCTCATCAATTTCTGCTTCTTCATGGAATGCTGACCTTCATTGCTCCATT
  TGTAGATTTTTGCTCTTCTCAGTTTACTCATTGTCCCCTGGAACAAATCACTGACATCTA
  CAACCATTACCATCTCACTAAATAAGACTTTCTATCCAATAATGATTGATACCTCAAATG
  TAAGATGCGTGATACTCAACATTTCATCGTCCACCTTCCCAACCCCAAACAATTCCATCT
  CGTTTCTTCTTGGTAAATGATGCTATGCTTTTTCCAACCAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000391
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000391.3, NP_000382.3
• RefSeq Size: 3540 bp
• RefSeq ORF: 1692 bp
• Locus ID: 1200
• Cytogenetics: 11p15.4
• Protein Families: Protease
• Protein Pathways: Lysosome
• Gene Summary: 'This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008]'