ATP6V0B (NM_001039457) Human Untagged Clone

CAT#: SC324389

ATP6V0B (untagged)-Human ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b (ATP6V0B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V0B
• Synonyms: ATP6F; HATPL; VMA16
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001039457.1 GTGGACGCTGGGACGCGTTTGTAGCTCCGGCCCCGCCGTTCCGACCCCCGCCGCCGTCGC
  CGCCATGACGGGGCTAGCACTGCTCTACTCCGGGGTCTTCGTGGCCTTCTGGGCCTGCGC
  GCTGGCCGTGGGAGTCTGCTACACCATTTTTGATTTGGGCTTCCGCTTTGATGTGGCATG
  GTTCCTGACGGAGACTTCGCCCTTCATGTGGTCCAACCTGGGCATTGGCCTAGCTATCTC
  CCTGTCTGTGGTTGGGGCAGCCTGGGGCATCTATATTACCGGCTCCTCCATCATTGGTGG
  AGGAGTGAAGGCCCCCAGGATCAAGACCAAGAACCTGGTCAGCATCATCTTCTGTGAGGC
  TGTGGCCATCTACGGCATCATCATGGCAATTGTCATTAGCAACATGGCTGAGCCTTTCAG
  TGCCACAGACCCCAAGGCCATCGGCCATCGGAACTACCATGCAGGCTACTCCATGTTTGG
  GGCTGGCCTCACCGTAGGCCTGTCTAACCTCTTCTGTGGAGTCTGCGTGGGCATCGTGGG
  CAGTGGGGCTGCCCTGGCCGATGCTCAGAACCCCAGCCTCTTTGTAAAGATTCTCATCGT
  GGAGATCTTTGGCAGCGCCATTGGCCTCTTTGGGGTCATCGTCGCAATTCTTCAGACCTC
  CAGAGTGAAGATGGGTGACTAGATGATATGTGTGGGTGGGGCCGTGCCTCACTTTTATTT
  ATTGCTGGTTTTCCTGGGACAGCTGGAGCTGTGTCCCTTAGCCTTTCAGAGGCTTGGTGT
  TCAGGGCCCTCCCTGCACTCCCCTCTTGCTGCGTGTTGATTTGGAGGCACTGCAGTCCAG
  GCCGAGTCCTCAGTGCGGGGAGCAGGCTGCTGCTGCTGACTCTGTGCAGCTGCGCACCTG
  TGTCCCCCACCTCCACCCTCAACCCATCTTCCTAGTGTTTGTGAAATAAACTTGGTATTT
  GTCTGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001039457.1 unedited
  GGCAACGCTGTATACGACTCCTATAGGGCGGCCGGGAATTCTCGGCACGAGGGTGGACGC
  TGGGACGCGTTTGTAGCTCCGGCCCCGCCGTTCCGACCCCCGCCGCCGTCGCCGCCATGA
  CGGGGCTAGCACTGCTCTACTCCGGGGTCTTCGTGGCCTTCTGGGCCTGCGCGCTGGCCG
  TGGGAGTCTGCTACACCATTTTTGATTTGGGCTTCCGCTTTGATGTGGCATGGTTCCTGA
  CGGAGACTTCGCCCTTCATGTGGTCCAACCTGGGCATTGGCCTAGCTATCTCCCTGTCTG
  TGGTTGGGGCAGCCTGGGGCATCTATATTACCGGCTCCTCCATCATTGGTGGAGGAGTGA
  AGGCCCCCAGGATCAAGACCAAGAACCTGGTCAGCATCATCTTCTGTGAGGCTGTGGCCA
  TCTACGGCATCATCATGGCAATTGTCATTAGCAACATGGCTGAGCCTTTCAGTGCCCAGA
  CCCCAAGGCCATCTGCGATCAGAAATACCATGCCAGC
• Restriction Sites: Please inquire
• ACCN: NM_001039457
• ORF Size: 477 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001039457.1, NP_001034546.1
• RefSeq Size: 979
• RefSeq ORF: 477
• Locus ID: 533
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: This gene encodes a portion of the V0 domain of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Activity of this enzyme is necessary for such varied processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
  Transcript Variant: This variant (2) lacks an alternate in-frame in the 5' coding region and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.