Prostate Specific Antigen (KLK3) (NM_001648) Human Untagged Clone

CAT#: SC324412

KLK3 (untagged)-Human kallikrein-related peptidase 3 (KLK3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KLK3
• Synonyms: APS; hK3; KLK2A1; PSA
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001648.2 AGCCCCAAGCTTACCACCTGCACCCGGAGAGCTGTGTCACCATGTGGGTCCCGGTTGTCT
  TCCTCACCCTGTCCGTGACGTGGATTGGCGCTGCGCCCCTCATCCTGTCTCGGATTGTGG
  GAGGCTGGGAGTGCGAGAAGCATTCCCAACCCTGGCAGGTGCTTGTGGCCTCTCGTGGCA
  GGGCAGTCTGCGGCGGTGTTCTGGTGCACCCCCAGTGGGTCCTCACAGCTGCCCACTGCA
  TCAGGAACAAAAGCGTGATCTTGCTGGGTCGGCACAGCTTGTTTCATCCTGAAGACACAG
  GCCAGGTATTTCAGGTCAGCCACAGCTTCCCACACCCGCTCTACGATATGAGCCTCCTGA
  AGAATCGATTCCTCAGGCCAGGTGATGACTCCAGCCACGACCTCATGCTGCTCCGCCTGT
  CAGAGCCTGCCGAGCTCACGGATGCTGTGAAGGTCATGGACCTGCCCACCCAGGAGCCAG
  CACTGGGGACCACCTGCTACGCCTCAGGCTGGGGCAGCATTGAACCAGAGGAGTTCTTGA
  CCCCAAAGAAACTTCAGTGTGTGGACCTCCATGTTATTTCCAATGACGTGTGTGCGCAAG
  TTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGCTGGACAGGGGGCAAAA
  GCACCTGCTCGGGTGATTCTGGGGGCCCACTTGTCTGTAATGGTGTGCTTCAAGGTATCA
  CGTCATGGGGCAGTGAACCATGTGCCCTGCCCGAAAGGCCTTCCCTGTACACCAAGGTGG
  TGCATTACCGGAAGTGGATCAAGGACACCATCGTGGCCAACCCCTGAGCACCCCTATCAA
  CTCCCTATTGTAGTAAACTTGGAACCTTGGAAATGACCAGGCCAAGACTCAAGCCTCCCC
  AGTTCTACTGACCTTTGTCCTTAGGTGTGAGGTCCAGGGTTGCTAGTAAAAGAAATCAGC
  AGACACAGGTGTAGACCAGAGTGTTTCTTAAATGGTGTAATTTTGTCCTCTCTGTGTCCT
  GGGGAATACTGGCCATGCCTGGAGACATATCACTCAATTTCTCTGAGGACACAGATAGGA
  TGGGGTGTCTGTGTTATTTGTGGGATACAGAGATGAAAGAGGGGTGGGATCCACACTGAG
  AGAGTGGAGAGTGACATGTGCTGGACACTGTCCATGAAGCACTGAGCAGAAGCTGGAGGC
  ACAACGCACCAGACACTCACAGCAAGGATGGAGCTGAAAACATAACCCACTCTGTCCTGG
  AGGCACTGGGAAGCCTAGAGAAGGCTGTGAGCCAAGGAGGGAGGGTCTTCCTTTGGCATG
  GGATGGGGATGAAGTAAGGAGAGGGACTGGACCCCCTGGAAGCTGATTCACTATGGGGGG
  AGGTGTATTGAAGTCCTCCAGACAACCCTCAGATTTGATGATTTCCTAGTAGAACTCACA
  GAAATAAAGAGCTGTTATACTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001648
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001648.2, NP_001639.1
• RefSeq Size: 1464 bp
• RefSeq ORF: 786 bp
• Locus ID: 354
• Cytogenetics: 19q13.33
• Domains: Tryp_SPc
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Pathways in cancer, Prostate cancer
• Gene Summary: 'Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease which is synthesized in the epithelial cells of the prostate gland, and is present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. The serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Dec 2019]'
  Transcript Variant: This variant (1) encodes the longest isoform (1) of this protein.