TFIP11 (NM_001008697) Human Untagged Clone

CAT#: SC324426

TFIP11 (untagged)-Human tuftelin interacting protein 11 (TFIP11), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TFIP11
• Synonyms: bK445C9.6; NTR1; Spp382; STIP; STIP-1; TIP39
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001008697.1 GCGTCTTCTCGCGCGGATGGTGAAGCTGAATTTGGTACGCTGGACCCTCGGACAGACAGC
  AAGAGAGAGACAAGCCCGAGGCCTGACTTCTAGCACTCCTGAGTTTGAGCCCTATGAGCA
  GTGGAACCTAGGGACCACCAAGGATCCAGATATAATTCTTTCCTCAGTAATAGAAAAAAG
  AATCTGGGATCTAAGAAGATACCTACTCATTTTCTAAGTGACTGGCCATGTCATTGTCCC
  ACTTATACCGGGATGGGGAAGGCCGCATTGATGATGATGATGACGAGCGGGAGAACTTTG
  AGATCACTGACTGGGATCTCCAGAATGAGTTCAACCCCAACCGACAGCGCCACTGGCAGA
  CCAAGGAAGAAGCCACCTACGGGGTGTGGGCAGAGCGAGACTCGGATGATGAGAGGCCCA
  GCTTTGGAGGCAAACGGGCCCGTGACTACTCTGCGCCAGTCAACTTCATCAGCGCAGGGC
  TCAAGAAAGGGGCAGCGGAGGAGGCAGAGTTGGAAGATTCTGATGACGAAGAGAAACCTG
  TTAAGCAGGACGACTTTCCTAAGGATTTTGGACCAAGGAAGCTAAAAACGGGTGGCAATT
  TTAAGCCCAGCCAGAAAGGTTTTGCAGGAGGAACCAAATCTTTCATGGACTTCGGCAGCT
  GGGAAAGACACACAAAAGGAATTGGACAGAAGCTTCTTCAGAAGATGGGCTACGTCCCTG
  GACGGGGCCTCGGGAAGAATGCACAAGGTATCATTAACCCAATTGAAGCCAAGCAGAGAA
  AGGGAAAAGGTGCTGTGGGGGCTTATGGATCCGAGCGCACCACTCAGTCCATGCAAGACT
  TCCCTGTGGTTGACTCAGAGGAAGAAGCTGAAGAGGAGTTTCAGAAGGAGCTGAGCCAGT
  GGAGGAAAGACCCAAGTGGAAGCAAGAAGAAGCCCAAATACTCTTACAAGACCGTGGAAG
  AGTTGAAGGCCAAGGGCAGGATTAGCAAGAAGCTCACTGCTCCCCAGAAGGAACTTTCTC
  AAGTCAAGGTCATAGACATGACAGGCCGGGAGCAGAAGGTCTACTACAGCTACAGTCAGA
  TCAGCCACAAGCACAACGTTCCCGATGATGGGCTGCCGCTACAGTCCCAACAGCTGCCAC
  AGTCTGGCAAAGAGGCCAAGGCCCCCGGCTTCGCGCTGCCCGAGCTGGAGCACAACCTGC
  AGCTGCTCATCGACCTCACGGAGCAGGAGATCATCCAGAATGACCGGCAGCTACAGTATG
  AGCGGGACATGGTGGTCAACCTCTTCCACGAGCTGGAGAAGATGACCGAGGTCCTGGACC
  ACGAGGAGCGGGTCATCTCGAACCTCAGCAAGGTCCTGGAGATGGTGGAGGAGTGCGAGC
  GGCGGATGCAGCCCGACTGCAGCAACCCCCTCACCCTGGACGAGTGTGCCCGCATCTTCG
  AAACCCTGCAGGACAAGTACTATGAGGAGTACAGGATGTCCGACCGTGTGGACCTTGCTG
  TGGCCATCGTCTATCCACTCATGAAGGAGTACTTCAAGGAGTGGGATCCCCTCAAAGACT
  GCACTTATGGCACCGAGATCATCTCTAAGTGGAAAAGCCTCCTAGAGAATGACCAGCTCT
  TGTCCCATGGCGGACAGGACCTCTCAGCAGATGCCTTTCACAGGTTGATATGGGAAGTCT
  GGATGCCTTTTGTTCGAAATATTGTCACCCAGTGGCAGCCAAGGAACTGTGACCCGATGG
  TGGACTTTTTGGATAGTTGGGTGCACATTATTCCTGTGTGGATCTTAGATAACATACTGG
  ACCAACTCATCTTCCCCAAGCTGCAAAAGGAGGTGGAAAACTGGAACCCGCTCACAGACA
  CTGTTCCCATCCACTCTTGGATCCACCCATGGCTGCCCCTTATGCAGGCACGGCTGGAGC
  CACTCTATTCCCCCATCCGTAGTAAGCTGTCCAGCGCCCTGCAGAAGTGGCACCCCAGCG
  ACTCCTCTGCCAAGCTCATCCTCCAGCCCTGGAAGGATGTCTTCACTCCTGGCTCCTGGG
  AAGCATTCATGGTCAAAAACATAGTGCCCAAGCTGGGGATGTGTCTTGGTGAGCTAGTCA
  TTAACCCCCACCAGCAGCACATGGATGCATTCTATTGGGTGATTGACTGGGAAGGGATGA
  TCTCTGTCTCTAGCCTGGTGGGACTTCTTGAAAAGCACTTCTTCCCCAAGTGGCTTCAGG
  TGCTGTGCTCTTGGCTCAGTAACAGCCCAAATTATGAGGAGATCACCAAGTGGTACCTGG
  GTTGGAAGTCGATGTTCTCAGACCAAGTGCTGGCACATCCATCTGTCAAGGACAAATTTA
  ATGAGGCACTTGATATCATGAACCGGGCGGTGTCCTCCAACGTTGGTGCCTACATGCAGC
  CAGGAGCACGGGAGAACATTGCCTATCTCACCCACACGGAGCGGAGGAAGGACTTCCAGT
  ACGAGGCCATGCAGGAGAGGCGGGAGGCTGAGAACATGGCTCAGAGGGGCATTGGCGTGG
  CCGCTAGCTCTGTGCCCATGAACTTTAAGGACCTCATTGAGACCAAGGCTGAGGAGCACA
  ACATTGTCTTCATGCCCGTCATTGGGAAGCGACACGAAGGGAAGCAGCTCTACACCTTTG
  GCCGCATTGTGATCTACATTGACCGGGGAGTGGTCTTTGTCCAGGGCGAGAAGACGTGGG
  TGCCCACCTCACTGCAGAGCCTGATCGACATGGCCAAGTGAACTGTGGCAGGTCCAGAAC
  CAGTCAGAGACTTGCCCCTAAGAGGGATGTACTGTAAATAAACAGTATTTTTAGATCACC
  TTCAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001008697
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008697.1, NP_001008697.1
• RefSeq Size: 2941 bp
• RefSeq ORF: 2514 bp
• Locus ID: 24144
• Cytogenetics: 22q12.1
• Gene Summary: This gene encodes a protein component of the spliceosome that promotes the release of the lariat-intron during late-stage splicing through the recruitment of a pre-mRNA splicing factor called DEAH-box helicase 15. The encoded protein contains a G-patch domain, a hallmark of RNA-processing proteins, that binds DEAH-box helicase 15. This protein contains an atypical nuclear localization sequence as well as a nuclear speckle-targeting sequence, enabling it to localize to distinct speckled regions within the cell nucleus. Polymorphisms in this gene are associated with dental caries suggesting a role in amelogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1-6 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.