Solute carrier family 22 member 5 (SLC22A5) (NM_003060) Human Untagged Clone

CAT#: SC324472

SLC22A5 (untagged)-Human solute carrier family 22 (organic cation/carnitine transporter), member 5 (SLC22A5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC22A5
• Synonyms: CDSP; OCTN2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003060.2 CCGCGGCAGGACCAAGGCGGCGGTGTCAGCTCGCGAGCCTACCCTCCGCGGACGGTCTTG
  GGTCGCCTGCTGCCTGGCTTGCCTGGTCGGCGGCGGGTGCCCCGCGCGCACGCGCAAAGC
  CCGCCGCGTTCCCCGACCCCAGGCCGCGCTCTGTGGGCCTCTGAGGGCGGCATGCGGGAC
  TACGACGAGGTGACCGCCTTCCTGGGCGAGTGGGGGCCCTTCCAGCGCCTCATCTTCTTC
  CTGCTCAGCGCCAGCATCATCCCCAATGGCTTCACCGGCCTGTCCTCCGTGTTCCTGATA
  GCGACCCCGGAGCACCGCTGCCGGGTGCCGGACGCCGCGAACCTGAGCAGCGCCTGGCGC
  AACCACACTGTCCCACTGCGGCTGCGGGACGGCCGCGAGGTGCCCCACAGCTGCCGCCGC
  TACCGGCTCGCCACCATCGCCAACTTCTCGGCGCTTGGGCTGGAGCCGGGGCGCGACGTG
  GACCTGGGGCAGCTGGAGCAGGAGAGCTGTCCGGATGGCTGGGAGTTCAGTCAGGACGTC
  TACCTGTCCACCATTGTGACCGAGTGGAACCTGGTGTGTGAGGACGACTGGAAGGCCCCA
  CTCACAATCTCCTTGTTCTTCGTGGGTGTGCTGTTGGGCTCCTTCATTTCAGGGCAGCTG
  TCAGACAGGTTTGGCCGGAAGAATGTGCTGTTCGTGACCATGGGCATGCAGACAGGCTTC
  AGCTTCCTGCAGATCTTCTCGAAGAATTTTGAGATGTTTGTCGTGCTGTTTGTCCTTGTA
  GGCATGGGCCAGATCTCCAACTATGTGGCAGCATTTGTCCTGGGGACAGAAATTCTTGGC
  AAGTCAGTTCGTATAATATTCTCTACGTTAGGAGTGTGCATATTTTATGCATTTGGCTAC
  ATGGTGCTGCCACTGTTTGCTTACTTCATCCGAGACTGGCGGATGCTGCTGGTGGCGCTG
  ACGATGCCGGGGGTGCTATGCGTGGCACTCTGGTGGTTCATCCCTGAGTCCCCCCGATGG
  CTCATCTCTCAGGGACGATTTGAAGAGGCAGAGGTGATCATCCGCAAGGCTGCCAAAGCC
  AATGGGATTGTTGTGCCTTCCACTATCTTTGACCCGAGTGAGTTACAAGACCTAAGTTCC
  AAGAAGCAGCAGTCCCACAACATTCTGGATCTGCTTCGAACCTGGAATATCCGGATGGTC
  ACCATCATGTCCATAATGCTGTGGATGACCATATCAGTGGGCTATTTTGGGCTTTCGCTT
  GATACTCCTAACTTGCATGGGGACATCTTTGTGAACTGCTTCCTTTCAGCGATGGTTGAA
  GTCCCAGCATATGTGTTGGCCTGGCTGCTGCTGCAATATTTGCCCCGGCGCTATTCCATG
  GCCACTGCCCTCTTCCTGGGTGGCAGTGTCCTTCTCTTCATGCAGCTGGTACCCCCAGAC
  TTGTATTATTTGGCTACAGTCCTGGTGATGGTGGGCAAGTTTGGAGTCACGGCTGCCTTT
  TCCATGGTCTACGTGTACACAGCCGAGCTGTATCCCACAGTGGTGAGAAACATGGGTGTG
  GGAGTCAGCTCCACAGCATCCCGCCTGGGCAGCATCCTGTCTCCCTACTTCGTTTACCTT
  GGTGCCTACGACCGCTTCCTGCCCTACATTCTCATGGGAAGTCTGACCATCCTGACAGCC
  ATCCTCACCTTGTTTCTCCCAGAGAGCTTCGGTACCCCACTCCCAGACACCATTGACCAG
  ATGCTAAGAGTCAAAGGAATGAAACACAGAAAAACTCCAAGTCACACAAGGATGTTAAAA
  GATGGTCAAGAAAGGCCCACAATCCTTAAAAGCACAGCCTTCTAACATCGCTTCCAGTAA
  GGGAGAAACTGAAGAGGAAAGACTGTCTTGCCAGAAATGGCCAGCTTGTGCAGACTCCGA
  GTCCTTCAGTGACAAAGGCCTTTGCTGTTTGTCCTCTTGACCTGTGTCTGACTTGCTCCT
  GGATGGGCACCCACACTCAGAGGCTACATATGGCCCTAGAGCACCACCTTCCTCTAGGGA
  CACTGGGGCTACCTACAGACAACTTCATCTAAGTCCTAACTATTACAATGATGGACTCAG
  CACCTCCAAAGCAGTTAATTTTTCACTAGAACCAGTGAGATCTGGAGGAATGTGAGAAGC
  ATATGCTAAATGTACATTTTAATTTTAGACTACTTGAAAAGGCCCCTAATAAGGCTAGAG
  GTCTAAGTCCCCCACCCCTTTCCCCACTCCCCTCTAGTGGTGAACTTTAGAGGAAAAGGA
  AGTAATTGCACAAGGAGTTTGATTCTTACCTTTTCTCAGTTACAGAGGACATTAACTGGA
  TCATTGCTTCCCCAGGGCAGGAGAGCGCAGAGCTAGGGAAAGTGAAAGGTAATGAAGATG
  GAGCAGAATGAGCAGATGCAGATCACCAGCAAAGTGCACTGATGTGTGAGCTCTTAAGAC
  CACTCAGCATGACGACTGAGTAGACTTGTTTACATCTGATCAAAGCACTGGGCTTGTCCA
  GGCTCATAATAAATGCTCCATTGAATCTACTATTCTTGTTTTCCACTGCTGTGGAAACCT
  CCTTGCTACTATAGCGTCTTATGTATGGTTTAAAGGAAATTTATCAGGTGAGAGAGATGA
  GCAACGTTGTCTTTTCTCTCAAAGCTGTAATGTGGGTTTTGTTTTACTGTTTATTTGTTT
  GTTGTTGTATCCTTTTCTCCTTGTTATTTGCCCTTCAGAATGCACTTGGGAAAGGCTGGT
  TCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003060
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003060.2, NP_003051.1
• RefSeq Size: 3252 bp
• RefSeq ORF: 1674 bp
• Locus ID: 6584
• Cytogenetics: 5q31.1
• Domains: sugar_tr
• Protein Families: Transmembrane
• Gene Summary: 'Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is a plasma integral membrane protein which functions both as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. The encoded protein is involved in the active cellular uptake of carnitine. Mutations in this gene are the cause of systemic primary carnitine deficiency (CDSP), an autosomal recessive disorder manifested early in life by hypoketotic hypoglycemia and acute metabolic decompensation, and later in life by skeletal myopathy or cardiomyopathy. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2015]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.