CTCF (NM_006565) Human Untagged Clone

CAT#: SC324590

CTCF (untagged)-Human CCCTC-binding factor (zinc finger protein) (CTCF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTCF
• Synonyms: MRD21
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006565.2 GTGGAGCGATTAAACCGTGCGCGGAGCTGCTTCTTTGGCGGCAGCGGCGGCGGCGGTGGC
  CGGTGCGGACGCGCGGAGCTCGCCGGAGACGCCGGGTGGCCGGAGCCGTGGAGCGGCGGC
  GGAGCGGGCGCCGCGGGGGGTGTGGCGCGGAGAATGATTACGGACCTGAAGCCAAAGAAC
  AAGATGCGCTAGTGGACAGATTGCTGACCAGGGGCTTGAGAGCTGGGTTCTATTTTCCCT
  CCTCAAACTGACTTTGCAGCCACGGAGAGGCAGGGGAAATGGAAGGTGATGCAGTCGAAG
  CCATTGTGGAGGAGTCCGAAACTTTTATTAAAGGAAAGGAGAGAAAGACTTACCAGAGAC
  GCCGGGAAGGGGGCCAGGAAGAAGATGCCTGCCACTTACCCCAGAACCAGACGGATGGGG
  GTGAGGTGGTCCAGGATGTCAACAGCAGTGTACAGATGGTGATGATGGAACAGCTGGACC
  CCACCCTTCTTCAGATGAAGACTGAAGTAATGGAGGGCACAGTGGCTCCAGAAGCAGAGG
  CTGCTGTGGACGATACCCAGATTATAACTTTACAGGTTGTAAATATGGAGGAACAGCCCA
  TAAACATAGGAGAACTTCAGCTTGTTCAAGTACCTGTTCCTGTGACTGTACCTGTTGCTA
  CCACTTCAGTAGAAGAACTTCAGGGGGCTTATGAAAATGAAGTGTCTAAAGAGGGCCTTG
  CGGAAAGTGAACCCATGATATGCCACACCCTACCTTTGCCTGAAGGGTTTCAGGTGGTTA
  AAGTGGGGGCCAATGGAGAGGTGGAGACACTAGAACAAGGGGAACTTCCACCCCAGGAAG
  ATCCTAGTTGGCAAAAAGACCCAGACTATCAGCCACCAGCCAAAAAAACAAAGAAAACCA
  AAAAGAGCAAACTGCGTTATACAGAGGAGGGCAAAGATGTAGATGTGTCTGTCTACGATT
  TTGAGGAAGAACAGCAGGAGGGTCTGCTATCAGAGGTTAATGCAGAGAAAGTGGTTGGTA
  ATATGAAGCCTCCAAAGCCAACAAAAATTAAAAAGAAAGGTGTAAAGAAGACATTCCAGT
  GTGAGCTTTGCAGTTACACGTGTCCACGGCGTTCAAATTTGGATCGTCACATGAAAAGCC
  ACACTGATGAGAGACCACACAAGTGCCATCTCTGTGGCAGGGCATTCAGAACAGTCACCC
  TCCTGAGGAATCACCTTAACACACACACAGGTACTCGTCCTCACAAGTGCCCAGACTGCG
  ACATGGCCTTTGTGACCAGTGGAGAATTGGTTCGGCATCGTCGTTACAAACACACCCACG
  AGAAGCCATTCAAGTGTTCCATGTGCGATTACGCCAGTGTAGAAGTCAGCAAATTAAAAC
  GTCACATTCGCTCTCATACTGGAGAGCGTCCGTTTCAGTGCAGTTTGTGCAGTTATGCCA
  GCAGGGACACATACAAGCTGAAAAGGCACATGAGAACCCATTCAGGGGAAAAGCCTTATG
  AATGTTATATTTGTCATGCTCGGTTTACCCAAAGTGGTACCATGAAGATGCACATTTTAC
  AGAAGCACACAGAAAATGTGGCCAAATTTCACTGTCCCCACTGTGACACAGTCATAGCCC
  GAAAAAGTGATTTGGGTGTCCACTTGCGAAAGCAGCATTCCTATATTGAGCAAGGCAAGA
  AATGCCGTTACTGTGATGCTGTGTTTCATGAGCGCTATGCCCTCATCCAGCATCAGAAGT
  CACACAAGAATGAGAAGCGCTTTAAGTGTGACCAGTGTGATTACGCTTGTAGACAGGAGA
  GGCACATGATCATGCACAAGCGCACCCACACCGGGGAGAAGCCTTACGCCTGCAGCCACT
  GCGATAAGACCTTCCGCCAGAAGCAGCTTCTCGACATGCACTTCAAGCGCTATCACGACC
  CCAACTTCGTCCCTGCGGCTTTTGTCTGTTCTAAGTGTGGGAAAACATTTACACGTCGGA
  ATACCATGGCAAGACATGCTGATAATTGTGCTGGCCCAGATGGCGTAGAGGGGGAAAATG
  GAGGAGAAACGAAGAAGAGTAAACGTGGAAGAAAAAGAAAGATGCGCTCTAAGAAAGAAG
  ATTCCTCTGACAGTGAAAATGCTGAACCAGATCTGGACGACAATGAGGATGAGGAGGAGC
  CTGCCGTAGAAATTGAACCTGAGCCAGAGCCTCAGCCTGTGACCCCAGCCCCACCACCCG
  CCAAGAAGCGGAGAGGACGACCCCCTGGCAGAACCAACCAGCCCAAACAGAACCAGCCAA
  CAGCTATCATTCAGGTTGAAGACCAGAATACAGGTGCAATTGAGAACATTATAGTTGAAG
  TAAAAAAAGAGCCAGATGCTGAGCCCGCAGAGGGAGAGGAAGAGGAGGCCCAGCCAGCTG
  CCACAGATGCCCCCAACGGAGACCTCACGCCCGAGATGATCCTCAGCATGATGGACCGGT
  GATGGCGGAGCCTTGTGCGTCGCCAGGACTTCTCTGGGCTGTGTTTAAACGGCCCGCATC
  TTAATTTTTCTCCCTTCTTTCTTTTTTTGGCTTTGGGAAAAGCATCATTTTACCAAACAT
  ACCGAGAACGAAAACTTCAAGGATGATGTTAGAAAAAAATGTGATTTAACTAGAACTTGC
  TGTCTGATGTTAGCAAATCATGGAATGTTCTGAGTCCCTGAGGGTTTACTGTGAAGTGCT
  GAGGACAGTGTTGACAACTAACTCGTTTTCCTAGATGGAAACGGAGACATTGACCCCTCC
  CTCCATGTGGTAAACCACTCCAGAATGGCCACCAGGCTTCCCAGAGTTCTATGGTCTTCT
  TCCCAAGAGAGTTTTTAATTGTAAATGCATACTTGGGAAGGACTTAGAGTTTTAAACTGT
  TTTTTGCTTTTGCTTTTCCCTGACTCCCTTTGCTTGGAGTCAGCTGCACACCAGTAGTAT
  GGCATGCTACGATCAGGTTCTGTCCTGAAAGCTTTGCCTCTTTCTTGGCAAAGTTTCTGG
  TATGGTCAAGCTTGTAAATAACTTTTTTTACATTTTAATCTTTTCCATTAATTAAGAGGT
  TGAAAAGAAGTGCAGTGTAAGAAAACCCAGCATTTTAATTACTTGCAAATTAAGTTACCA
  CAGACTCTGTAGTGTGTAAATGTTGACAAGGAATTGGATCACAATCATGTAGCAGAATGG
  CACCCAGACCACTGCCCACCAGTGACGGACATGCACGTGGCAGATCATGATTTCCAGCCC
  ACGGAGCCAGCATTTGAACCTTGTATAATTAACTTTCAGTTATGATTTCCCATCGACATT
  TTCTTTGCCCTGTTTGTAGCTGATTGTTGTGTTTTATAAATCTTCTGTTAAGGCAGAAGG
  GTGATTATGAGTGGTTCACAGCAGCCCTTATAAGCTGGGCCAGAAAATTTCACTAGGTCA
  GTAATTTAAACCTTGGATCTTCAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006565.2 unedited
  GGCGATCGTATACGACTCCTATAGGGCGGCCGGGAATCGGCACGAGGGTGGAGCGATTAA
  ACCGTGCGCGGAGCTGCTTCTTTGGCGGCAGCGGCGGCGGCGGTGGCCGGTGCGGACGCG
  CGGAGCTCGCCGGAGACGCCGGGTGGCCGGAGCCGTGGAGCGGCGGCGGAGCGGGCGCCG
  CGGGGGGTGTGGCGCGGAGAATGATTACGGACCTGAAGCCAAAGAACAAGATGCGCTAGT
  GGACAGATTGCTGACCAGGGGCTTGAGAGCTGGGTTCTATTTTCCCTCCTCAAACTGACT
  TTGCAGCCACGGAGAGGCAGGGGAAATGGAAGGTGATGCAGTCGAAGCCATTGTGGAGGA
  GTCCGAAACTTTTATTAAAGGAAAGGAGAGAAAGACTTACCAGAGACGCCGGGAAGGGGG
  CCAGGAAGAAGATGCCTGCCACTTACCCCAGAACCAGACGGATGGGGGTGAGGTGGTCCA
  GGATGTCAACAGCAGTGTACAGATGGTGATGATGGAACAGCTGGACCCCACCCTTCTTCA
  GATGAAGACTGAAGTAATGGAGGGCACAGTGGCTCCAGAAGCAGAGGCTGCTGTGGACGA
  TACCCAGATTATAACTTTACAGGTTGTAAATATGGAGGAACAGCCCATAAACATAGGAGA
  ACTTCAGCTTGTTCAAGTACCTGTTCCTGTGACTGTACCTGTTGCTACCACTTCAGTAGA
  AGAACTTCAGGGGCTTATTGAAAATGAAGTGTCTAATGAAGGCCTTGCTGAAAGTTGAAC
  CCATGATATGCCCACCCTACCTTTTGCCTGAAGGTTTCAGGTTGTTAAATGTGGGGGCCT
  AATGATATAGTTGATACCCTAGACTATGGTGGCACTTCCACTCCATGGAGGATCTCTAGC
  TTGGATAACGAGCACTCGATCTCTAGT
• Restriction Sites: SgfI-XhoI
• ACCN: NM_006565
• ORF Size: 2184 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_006565.2, NP_006556.1
• RefSeq Size: 3797
• RefSeq ORF: 2184
• Locus ID: 10664
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) is the longer transcript and encodes the longer isoform (1).