IL1 alpha (IL1A) (NM_000575) Human Untagged Clone

CAT#: SC324639

IL1A (untagged)-Human interleukin 1, alpha (IL1A)


  "NM_000575" in other vectors (6)

Reconstitution Protocol

USD 660.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol IL1A
Synonyms IL-1 alpha; IL-1A; IL1; IL1-ALPHA; IL1F1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_000575.3 GTCATTTCATTGGCGTTTGAGTCAGCAAAGAAGTCAAGATGGCCAAAGTTCCAGACATGT
TTGAAGACCTGAAGAACTGTTACAGTGAAAATGAAGAAGACAGTTCCTCCATTGATCATC
TGTCTCTGAATCAGAAATCCTTCTATCATGTAAGCTATGGCCCACTCCATGAAGGCTGCA
TGGATCAATCTGTGTCTCTGAGTATCTCTGAAACCTCTAAAACATCCAAGCTTACCTTCA
AGGAGAGCATGGTGGTAGTAGCAACCAACGGGAAGGTTCTGAAGAAGAGACGGTTGAGTT
TAAGCCAATCCATCACTGATGATGACCTGGAGGCCATCGCCAATGACTCAGAGGAAGAAA
TCATCAAGCCTAGGTCAGCACCTTTTAGCTTCCTGAGCAATGTGAAATACAACTTTATGA
GGATCATCAAATACGAATTCATCCTGAATGACGCCCTCAATCAAAGTATAATTCGAGCCA
ATGATCAGTACCTCACGGCTGCTGCATTACATAATCTGGATGAAGCAGTGAAATTTGACA
TGGGTGCTTATAAGTCATCAAAGGATGATGCTAAAATTACCGTGATTCTAAGAATCTCAA
AAACTCAATTGTATGTGACTGCCCAAGATGAAGACCAACCAGTGCTGCTGAAGGAGATGC
CTGAGATACCCAAAACCATCACAGGTAGTGAGACCAACCTCCTCTTCTTCTGGGAAACTC
ACGGCACTAAGAACTATTTCACATCAGTTGCCCATCCAAACTTGTTTATTGCCACAAAGC
AAGACTACTGGGTGTGCTTGGCAGGGGGGCCACCCTCTATCACTGACTTTCAGATACTGG
AAAACCAGGCGTAGGTCTGGAGTCTCACTTGTCTCACTTGTGCAGTGTTGACAGTTCATA
TGTACCATGTACATGAAGAAGCTAAATCCTTTACTGTTAGTCATTTGCTGAGCATGTACT
GAGCCTTGTAATTCTAAATGAATGTTTACACTCTTTGTAAGAGTGGAACCAACACTAACA
TATAATGTTGTTATTTAAAGAACACCCTATATTTTGCATAGTACCAATCATTTTAATTAT
TATTCTTCATAACAATTTTAGGAGGACCAGAGCTACTGACTATGGCTACCAAAAAGACTC
TACCCATATTACAGATGGGCAAATTAAGGCATAAGAAAACTAAGAAATATGCACAATAGC
AGTTGAAACAAGAAGCCACAGACCTAGGATTTCATGATTTCATTTCAACTGTTTGCCTTC
TACTTTTAAGTTGCTGATGAACTCTTAATCAAATAGCATAAGTTTCTGGGACCTCAGTTT
TATCATTTTCAAAATGGAGGGAATAATACCTAAGCCTTCCTGCCGCAACAGTTTTTTATG
CTAATCAGGGAGGTCATTTTGGTAAAATACTTCTTGAAGCCGAGCCTCAAGATGAAGGCA
AAGCACGAAATGTTATTTTTTAATTATTATTTATATATGTATTTATAAATATATTTAAGA
TAATTATAATATACTATATTTATGGGAACCCCTTCATCCTCTGAGTGTGACCAGGCATCC
TCCACAATAGCAGACAGTGTTTTCTGGGATAAGTAAGTTTGATTTCATTAATACAGGGCA
TTTTGGTCCAAGTTGTGCTTATCCCATAGCCAGGAAACTCTGCATTCTAGTACTTGGGAG
ACCTGTAATCATATAATAAATGTACATTAATTACCTTGAGCCAGTAATTGGTCCGATCTT
TGACTCTTTTGCCATTAAACTTACCTGGGCATTCTTGTTTCAATTCCACCTGCAATCAAG
TCCTACAAGCTAAAATTAGATGAACTCAACTTTGACAACCATGAGACCACTGTTATCAAA
ACTTTCTTTTCTGGAATGTAATCAATGTTTCTTCTAGGTTCTAAAAATTGTGATCAGACC
ATAATGTTACATTATTATCAACAATAGTGATTGATAGAGTGTTATCAGTCATAACTAAAT
AAAGCTTGCAACAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_000575
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000575.3, NP_000566.3
RefSeq Size 2943 bp
RefSeq ORF 816 bp
Locus ID 3552
Cytogenetics 2q14.1
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Apoptosis, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, Prion diseases, Type I diabetes mellitus
Gene Summary 'The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]'

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.