IL1 alpha (IL1A) (NM_000575) Human Untagged Clone

CAT#: SC324639

IL1A (untagged)-Human interleukin 1, alpha (IL1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL1A
• Synonyms: IL-1 alpha; IL-1A; IL1; IL1-ALPHA; IL1F1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000575.3 GTCATTTCATTGGCGTTTGAGTCAGCAAAGAAGTCAAGATGGCCAAAGTTCCAGACATGT
  TTGAAGACCTGAAGAACTGTTACAGTGAAAATGAAGAAGACAGTTCCTCCATTGATCATC
  TGTCTCTGAATCAGAAATCCTTCTATCATGTAAGCTATGGCCCACTCCATGAAGGCTGCA
  TGGATCAATCTGTGTCTCTGAGTATCTCTGAAACCTCTAAAACATCCAAGCTTACCTTCA
  AGGAGAGCATGGTGGTAGTAGCAACCAACGGGAAGGTTCTGAAGAAGAGACGGTTGAGTT
  TAAGCCAATCCATCACTGATGATGACCTGGAGGCCATCGCCAATGACTCAGAGGAAGAAA
  TCATCAAGCCTAGGTCAGCACCTTTTAGCTTCCTGAGCAATGTGAAATACAACTTTATGA
  GGATCATCAAATACGAATTCATCCTGAATGACGCCCTCAATCAAAGTATAATTCGAGCCA
  ATGATCAGTACCTCACGGCTGCTGCATTACATAATCTGGATGAAGCAGTGAAATTTGACA
  TGGGTGCTTATAAGTCATCAAAGGATGATGCTAAAATTACCGTGATTCTAAGAATCTCAA
  AAACTCAATTGTATGTGACTGCCCAAGATGAAGACCAACCAGTGCTGCTGAAGGAGATGC
  CTGAGATACCCAAAACCATCACAGGTAGTGAGACCAACCTCCTCTTCTTCTGGGAAACTC
  ACGGCACTAAGAACTATTTCACATCAGTTGCCCATCCAAACTTGTTTATTGCCACAAAGC
  AAGACTACTGGGTGTGCTTGGCAGGGGGGCCACCCTCTATCACTGACTTTCAGATACTGG
  AAAACCAGGCGTAGGTCTGGAGTCTCACTTGTCTCACTTGTGCAGTGTTGACAGTTCATA
  TGTACCATGTACATGAAGAAGCTAAATCCTTTACTGTTAGTCATTTGCTGAGCATGTACT
  GAGCCTTGTAATTCTAAATGAATGTTTACACTCTTTGTAAGAGTGGAACCAACACTAACA
  TATAATGTTGTTATTTAAAGAACACCCTATATTTTGCATAGTACCAATCATTTTAATTAT
  TATTCTTCATAACAATTTTAGGAGGACCAGAGCTACTGACTATGGCTACCAAAAAGACTC
  TACCCATATTACAGATGGGCAAATTAAGGCATAAGAAAACTAAGAAATATGCACAATAGC
  AGTTGAAACAAGAAGCCACAGACCTAGGATTTCATGATTTCATTTCAACTGTTTGCCTTC
  TACTTTTAAGTTGCTGATGAACTCTTAATCAAATAGCATAAGTTTCTGGGACCTCAGTTT
  TATCATTTTCAAAATGGAGGGAATAATACCTAAGCCTTCCTGCCGCAACAGTTTTTTATG
  CTAATCAGGGAGGTCATTTTGGTAAAATACTTCTTGAAGCCGAGCCTCAAGATGAAGGCA
  AAGCACGAAATGTTATTTTTTAATTATTATTTATATATGTATTTATAAATATATTTAAGA
  TAATTATAATATACTATATTTATGGGAACCCCTTCATCCTCTGAGTGTGACCAGGCATCC
  TCCACAATAGCAGACAGTGTTTTCTGGGATAAGTAAGTTTGATTTCATTAATACAGGGCA
  TTTTGGTCCAAGTTGTGCTTATCCCATAGCCAGGAAACTCTGCATTCTAGTACTTGGGAG
  ACCTGTAATCATATAATAAATGTACATTAATTACCTTGAGCCAGTAATTGGTCCGATCTT
  TGACTCTTTTGCCATTAAACTTACCTGGGCATTCTTGTTTCAATTCCACCTGCAATCAAG
  TCCTACAAGCTAAAATTAGATGAACTCAACTTTGACAACCATGAGACCACTGTTATCAAA
  ACTTTCTTTTCTGGAATGTAATCAATGTTTCTTCTAGGTTCTAAAAATTGTGATCAGACC
  ATAATGTTACATTATTATCAACAATAGTGATTGATAGAGTGTTATCAGTCATAACTAAAT
  AAAGCTTGCAACAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000575
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000575.3, NP_000566.3
• RefSeq Size: 2943 bp
• RefSeq ORF: 816 bp
• Locus ID: 3552
• Cytogenetics: 2q14.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, Prion diseases, Type I diabetes mellitus
• Gene Summary: 'The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]'