MYL12B (NM_001144944) Human Untagged Clone

CAT#: SC324759

MYL12B (untagged)-Human myosin, light chain 12B, regulatory (MYL12B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYL12B
• Synonyms: MLC-B; MRLC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001144944 edited
  GGTGTTTGGCGTCGGAATTAAACAACCACCATGTCGAGCAAAAAGGCAAAGACCAAGACC
  ACCAAGAAGCGCCCTCAGCGTGCAACATCCAATGTGTTTGCCATGTTTGACCAGTCACAG
  ATTCAGGAGTTCAAAGAGGCCTTCAACATGATTGATCAGAACAGAGATGGCTTCATCGAC
  AAGGAAGATTTGCATGATATGCTTGCTTCTCTAGGGAAGAATCCCACTGATGCATACCTT
  GATGCCATGATGAATGAGGCCCCAGGGCCCATCAATTTCACCATGTTCCTGACCATGTTT
  GGTGAGAAGTTAAATGGCACAGATCCTGAAGATGTCATCAGAAACGCCTTTGCTTGCTTT
  GATGAAGAAGCAACAGGCACCATTCAGGAAGATTACCTAAGAGAGCTGCTGACAACCATG
  GGGGATCGGTTTACAGATGAGGAAGTGGATGAGCTGTACAGAGAAGCACCTATTGACAAA
  AAGGGGAATTTCAATTACATCGAGTTCACACGCATCCTGAAACATGGAGCCAAAGACAAA
  GATGACTGAAAGAACTTTAGCTAAAATCTTCCAGTTACATTGTCTTACTCTCTTTTACTT
  CTCAGACACTTCCCCCACCCTCATAGAACCTGTTGCATGCAACTTAGTTTCACAGCTTTG
  CCTCTTCTTTTTGATGTATTTATTCCAGACCTTTCTGCCACTTAGCACTTGTATAATCAG
  ACTGGAAATGGGGATGAGGGTGTAAATTGTATTGAAAAAGATCGCGAATAAAAATCAACA
  AATGTGAAAGCCCAGAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006471 unedited
  TATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGGTGTTTGGCGTCGGAATTA
  AACAACCACCATGTCGAGCAAAAAGGCAAAGACCAAGACCACCAAGAAGCGCCCTCAGCG
  TGCAACATCCAATGTGTTTGCCATGTTTGACCAGTCACAGATTCAGGAGTTCAAAGAGGC
  CTTCAACATGATTGATCAGAACAGAGATGGCTTCATCGACAAGGAAGATTTGCATGATAT
  GCTTGCTTCTCTAGGGAAGAATCCCACTGATGCATACCTTGATGCCATGATGAATGAGGC
  CCCAGGGCCCATCAATTTCACCATGTTCCTGACCATGTTTGGTGAGAAGTTAAATGGCAC
  AGATCCTGAAGATGTCATCAGAAACGCCTTTGCTTGCTTTGATGAAGAAGCAACAGGCAC
  CATTCAGGAAGATTACCTAAGAGAGCTGCTGACAACCATGGGGNGATCGGTTTACAGATG
  AGGAAGTGGATGAGCTGTACAGAGAAGCACCTATTGACAAAAAGGGGAATTTCAATTACA
  TCGAGTTCACACGCATCCTGAAACATGGAGCCAAAGACAAAGATGACTGAAAGAACTTTA
  GCTAAAATCTTCCAGTTACATTGTCTTACTCTCTTTTACTTCTCAGACACTTCCCCCACC
  CTCATAGAACCTGTTGCATGCAACTTAGTTTCACAGCTTTGCCTCTTCTTTTTGATGTAT
  TTATTCCAGACCTTTCTGCCACTTAGCACTTGTATAATCAGACTGGAAATGGNGATTGAG
  GNTGTAAATTGTATTGAAAAAAGATCGCGAATAAAAATCAACAATGTGNAAAGCCCCCAG
  AAAAANAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATTAGCTGTTTCTGAACGA
  TACCGGNTTGGCATCCTTGTGAACCCTCCCAGTGCCTTTCCTGGNCCTGAAAGNTGCCAC
  TCCATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006471 unedited
  TGGTATGGCAACTTCCAGGGCCAGTANAGGCACTGGGGNAGGGTCACAGGGATGCCACCC
  GGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTT
  TTTTTTTTCTGGGCTTTCACATTTGTTGATTTTTATTCGCGATCTTTTTCAATACAATTT
  ACACCCTCATCCCCATTTCCAGTCTGATTATACAAGTGCTAAGTGGCAGAAAGGTCTGGA
  ATAAATACATCAAAAAGAAGAGGCAAAGCTGTGAAACTAAGTTGCATGCAACAGGTTCTA
  TGAGGGTGGGGGAAGTGTCTGAGAAGTAAAAGAGAGTAAGACAATGTAACTGGAAGATTT
  TAGCTAAAGTTCTTTCAGTCATCTTTGTCTTTGGCTCCATGTTTCAGGATGCGTGTGAAC
  TCGATGTAATTGAAATTCCCCTTTTTGTCAATAGGTGCTTCTCTGTACAGCTCATCCACT
  TCCTCATCTGTAAACCGATCCCCCATGGTTGTCAGCAGCTCTCTTAGGTAATCTTCCTGA
  ATGGTGCCTGTTGCTTCTTCATCAAAGCAAGCAAAGGCGTTTCTGATGACATCTTCAGGA
  TCTGTGCCATTTAACTTCTCACCAAACATGGTCAGGAACATGGTGAAATTGATGGGCCCT
  GNGGCCTCATTCATCATGGCATCAAGGTATGCATCAGTGGGATTCTTCCCTAGAGAGCAG
  CATATATGCANATCTTCCTTGTCGATGAACCATCTCTGTTCTGATCAATCATGTTGAGGC
  CTCTTTTGACTCCTGAATCTGTGACTGGTCAACATGGCANACACATTGNATGGTGCCGCT
  GAGGCGCTTCTTGGNTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001144944
• ORF Size: 519 bp
• Insert Size: 800
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001144944.1, NP_001138416.1
• RefSeq Size: 1041
• RefSeq ORF: 519
• Locus ID: 103910
• Protein Pathways: Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction
• Gene Summary: The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1-3 all encode the same protein.