NFKBIL1 (NM_001144963) Human Untagged Clone

CAT#: SC324972

NFKBIL1 (untagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 (NFKBIL1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFKBIL1
• Synonyms: IKBL; NFKBIL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001144963, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCTCCACTTCCCGCCGCCAACGCCGAGAACGTCGCTTTCGTCGTTACTTGTCTGCAGGACGGCTGG
  TCCGGGCCCAGGCCCTCCTCCAGCGACACCCAGGCCTCGATGTAGATGCTGGGCAGCCCCCACCACTGCA
  CCGGGCCTGTGCCCGCCACGATGCCCCTGCCCTGTGCCTGCTGCTTCGGCTCGGGGCTGACCCTGCCCAC
  CAGGACCGCCATGGGGACACGGCACTGCATGCTGCTGCCCGCCAGGGCCCAGATGCCTACACCGATTTCT
  TCCTCCCGCTGCTAAGCCGCTGTCCCTCCGCCATGGGAATAAAGAATAAGGATGGGGAGACCCCTGGCCA
  AATTTTGGGCTGGGGACCCCCCTGGGATTCTGCTGAAGAGGAGGAAGAAGATGATGCCTCCAAGGAGCGG
  GAATGGAGACAGAAGCTCCAGGGTGATGCCTCCCATGAAACCCAGGAACCTGAGTCCTTCTCAGCCTGGT
  CAGATCGCCTGGCCCGGGAACATGCCCAGAAGTGCCAGCAGCAGCAGCGAGAAGCAGAGGGATCCCGTCG
  ACCCCCACGTGCTGAGGGCTCCAGCCAGAGCTGGCGACAGCAGGAGGAGGAGCAGCGGCTCTTCAGGGAG
  CGAGCCCGGGCCAAGGAGGAAGAGCTGCGTGAGAGCCGAGCCAGGAGGGCGCAGGAGGCTCTAGGGGACC
  GAGAACCCAAGCCAACCAGGGCCGGGCCCAGGGAAGAGCACCCCAGAGGAGCGGGGAGGGGCAGCCTCTG
  GCGATTTGGTGATGTGCCCTGGCCCTGCCCTGGGGGAGGGGACCCAGAGGCCATGGCTGCAGCCCTGGTG
  GCCAGGGGCCCCCCTTTGGAGGAACAGGGGGCTCTGAGGAGGTACTTGAGGGTCCAGCAGGTCCGCTGGC
  ACCCTGACCGCTTCCTGCAGCGATTCCGAAGCCAGATTGAGACCTGGGAGCTGGGCCGTGTGATGGGAGC
  AGTGACAGCCCTTTCTCAGGCCCTGAATCGCCATGCAGAGGCCCTCAAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001144963
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144963.1, NP_001138435.1
• RefSeq Size: 1412 bp
• RefSeq ORF: 1032 bp
• Locus ID: 4795
• Cytogenetics: 6p21.33
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a divergent member of the I-kappa-B family of proteins. Its function has not been determined. The gene lies within the major histocompatibility complex (MHC) class I region on chromosome 6. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]'
  Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (4) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform 1.