PBX3 (NM_001134778) Human Untagged Clone

CAT#: SC324994

PBX3 (untagged)-Human pre-B-cell leukemia homeobox 3 (PBX3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PBX3
• Synonyms: OTTHUMP00000022145; OTTHUMP00000064213; pre-B-cell leukemia homeobox 3; pre-B-cell leukemia transcription factor 3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001134778, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAACCAGCGCTCTTCAGCGTCCTGTGTGAGATCAAAGAGAAAACAGGTCTCAGCATCAGAGGAGCCC
  AGGAGGAGGACCCTCCCGATCCCCAGCTAATGAGACTGGACAATATGCTTTTGGCAGAAGGGGTTTCAGG
  TCCTGAGAAAGGTGGGGGATCGGCGGCAGCAGCTGCAGCCGCGGCAGCCTCTGGAGGTTCTTCAGATAAC
  TCTATTGAACACTCAGATTACAGAGCCAAATTGACCCAGATCAGACAAATCTATCACACAGAACTGGAGA
  AATATGAACAGGCATGTAATGAATTTACTACACATGTGATGAACCTTCTCCGAGAACAGAGTAGAACACG
  TCCCATTTCTCCAAAAGAGATTGAAAGAATGGTGGGCATCATCCATCGAAAATTTAGTTCCATTCAGATG
  CAGCTCAAACAAAGCACTTGTGAAGCAGTTATGATTTTAAGATCAAGGTTCCTTGATGCCAGACGGAAAA
  GGCGTAACTTCAGTAAACAGGCCACAGAAATCTTGAATGAATATTTTTACTCACACCTCAGCAACCCCTA
  CCCCAGTGAAGAAGCCAAAGAGGAGCTGGCCAAGAAATGCAGCATCACAGTGTCACAGGTATCCAATTGG
  TTTGGCAACAAACGAATCAGGTACAAGAAGAACATTGGCAAGTTTCAGGAAGAAGCCAACCTCTATGCTG
  CAAAGACGGCCGTGACAGCTGCACACGCAGTAGCAGCAGCTGTGCAGAACAACCAGACCAATTCGCCCAC
  CACACCAAATTCCGGTTCTTCTGGTTCTTTTAACCTCCCAAATTCTGGGGACATGTTCATGAACATGCAG
  AGTCTGAATGGGGATTCTTACCAAGGGTCCCAAGTCGGAGCCAATGTGCAATCACAGGTGGATACCCTCC
  GTCATGTTATCAATCAGACGGGAGGCTACAGTGATGGCCTTGGAGGAAATTCACTGTACAGTCCACATAA
  TTTAAATGCTAATGGAGGCTGGCAGGACGCAACAACTCCATCTTCTGTGACTTCTCCTACAGAAGGCCCA
  GGAAGTGTGCACTCGGATACCTCTAACTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001134778
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134778.1, NP_001128250.1
• RefSeq Size: 2825 bp
• RefSeq ORF: 1080 bp
• Locus ID: 5090
• Cytogenetics: 9q33.3
• Protein Families: Transcription Factors
• Gene Summary: ''
  Transcript Variant: This variant (2) utilizes an alternate first exon and 5'UTR; thus, the first likely translation initiation site corresponds to an internal methionine in variant 1. The predicted protein (isoform 2) is N-terminally truncated compared to isoform 1 and is not predicted to have a signal peptide.