SPHK1 (NM_001142602) Human Untagged Clone

CAT#: SC325028

SPHK1 (untagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPHK1
• Synonyms: SPHK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021972, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCCAGTGGTCGGTTGCGGACGTGGCCTCTTTGGTTTTGTTTTCTCAGCGGGCGGCCCCCGGGGCG
  TGCTCCCGCGGCCCTGCCGCGTGCTGGTGCTGCTGAACCCGCGCGGCGGCAAGGGCAAGGCCTTGCAGCT
  CTTCCGGAGTCACGTGCAGCCCCTTTTGGCTGAGGCTGAAATCTCCTTCACGCTGATGCTCACTGAGCGG
  CGGAACCACGCGCGGGAGCTGGTGCGGTCGGAGGAGCTGGGCCGCTGGGACGCTCTGGTGGTCATGTCTG
  GAGACGGGCTGATGCACGAGGTGGTGAACGGGCTCATGGAGCGGCCTGACTGGGAGACCGCCATCCAGAA
  GCCCCTGTGTAGCCTCCCAGCAGGCTCTGGCAACGCGCTGGCAGCTTCCTTGAACCATTATGCTGGCTAT
  GAGCAGGTCACCAATGAAGACCTCCTGACCAACTGCACGCTATTGCTGTGCCGCCGGCTGCTGTCACCCA
  TGAACCTGCTGTCTCTGCACACGGCTTCGGGGCTGCGCCTCTTCTCTGTGCTCAGCCTGGCCTGGGGCTT
  CATTGCTGATGTGGACCTAGAGAGTGAGAAGTATCGGCGTCTGGGGGAGATGCGCTTCACTCTGGGCACC
  TTCCTGCGTCTGGCAGCCCTGCGCACCTACCGCGGCCGACTGGCCTACCTCCCTGTAGGAAGAGTGGGTT
  CCAAGACACCTGCCTCCCCCGTTGTGGTCCAGCAGGGCCCGGTAGATGCACACCTTGTGCCACTGGAGGA
  GCCAGTGCCCTCTCACTGGACAGTGGTGCCCGACGAGGACTTTGTGCTAGTCCTGGCACTGCTGCACTCG
  CACCTGGGCAGTGAGATGTTTGCTGCACCCATGGGCCGCTGTGCAGCTGGCGTCATGCATCTGTTCTACG
  TGCGGGCGGGAGTGTCTCGTGCCATGCTGCTGCGCCTCTTCCTGGCCATGGAGAAGGGCAGGCATATGGA
  GTATGAATGCCCCTACTTGGTATATGTGCCCGTGGTCGCCTTCCGCTTGGAGCCCAAGGATGGGAAAGGT
  GTGTTTGCAGTGGATGGGGAATTGATGGTTAGCGAGGCCGTGCAGGGCCAGGTGCACCCAAACTACTTCT
  GGATGGTCAGCGGTTGCGTGGAGCCCCCGCCCAGCTGGAAGCCCCAGCAGATGCCACCGCCAGAAGAGCC
  CTTATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021972 unedited
  NNNNGGCGGTTTAGGTTTTGTATACGACTCATATAGGCGGCCGCGCAATTCGCACCAGGC
  GAGGCCGGGGAGTCCGCTCCACGGGGCGCTCCAGTCCCTCAGACGTGGGCTGAGCTTGGG
  ACGAGCTGCGTTCCGCCCCAGGCCACTGTAGGGAACGGCGGTGGCGCCTCCCCAGCAAAC
  CGGACCGACTGGGTCCAGCCGCCGCAGGGAATGACGCCGGTGCTCCTACAGCCACGGCTC
  CGGGCGGGGAAGGCGAGCCCCACAGCCGGCCCTGCGACGCCCGCCTGGGCAGCACCGATA
  AGGAGCTGAAGGCAGGAGCCGCCGCCACGGGCAGCGCCCCCACAGCGCCAGGGACCCCCT
  GGCAGCGGGAGCCGCGGGTCGAGGTTATGGATCCAGCGGGCGGCCCCCGGGGCGTGCTCC
  CGCGGCCCTGCCGCGTGCTGGTGCTGCTGAACCCGCGCGGCGGCAAGGGCAAGGCCTTGC
  AGCTCTTCCGGAGTCACGTGCAGCCCCTTTTGGCTGAGGCTGAAATCTCCTTCACGCTGA
  TGCTCACTGAGCGGCGGAACCACGCGCGGGAGCTGGTGCGGTCGGAGGAGCTGGGCCGCT
  GGGACGCTCTGGTGGTCATGTCTGGAGACGGGCTGATGCACGAGGTGGTGAACGGGCTCA
  TGGAGCGGCCTGACTGGGAGACCGCCATCCAGAAGCCCCTGTGTAGCCTCCCAGCAGGCT
  CTGGCAACGCGCTGGCAGGTTCCTTGAACCATTATGCTGGCTATGAGCAGGTCACCAATG
  AAGACCTCCTGACCAACTGCACGCTATTGCTGTGCCGCCGGCTGCTGTCACCCATGAACC
  TGCTGTCTCTGCACACGGCTTCGGGGCTGCGCCTCTTCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021972 unedited
  GTTGACCTTGGCGATGGCACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGAT
  GCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGCTCGAG
  TTTTTTTTTTTTTTTTTGGGAATGTCACTTTATTTGGATTTGGTTCGTGGGGTGGGGGTC
  TCAAAACAAACTAGAAGGCCTTACATAGGCAGCTGGGCCCAGCCAGCTGGGCTCCTGACC
  CAAGACTTCATTCTGGCCTGTCCCCCCAAAGCATAGCCTCCACCTTCTCACCCTTCTCCA
  GAGGAGTCTCCTCCACCCCCACAGGAGCTGTGGACAGGCCCTGCAGCCCTAGGGAAGGAG
  GAAGGGTCCTGCAAGTAGACACTAAGGCACAGCGCGGCCCAGGGGTCATAAGGGCTCTTC
  TGGCGGTGGCATCTGCTGGGGCTTCCAGCTGGGCGGGGGCTCCACGCAACCGCTGACCAT
  CCAGAAGTAGTTTGGGTGCACCTGGCCCTGCACGGCCTCGCTAACCATCAATTCCCCATC
  CACTGCAAACACACCTTTCCCATCCTTGGGCTCCAAGCGGAAGGCGACCACGGGCACGTA
  TACCAAGTAGGGGCATTCATACTCCATATGCCTGCCCTTCTCCATGGCCAGGAAGAGGCG
  CAGCAGCATGGCACGAGACACTCCCGCCCGCACGTAGAACAGATGCATGACGCCAGCTGC
  ACAGCGGCCCATGGGTGCAGCAAACATCTCACTGCCCAGGTGCGAGTGCAGCAGTGCCAG
  GACTAGCACAAAGTCCTCGTCGGGCACCACTGTCCAGTGAGAGGGCACTGGCTCCTCCAG
  TGGCACAAGGTGTGCATCTACCGGGCCCCTGCTGACACACGGGGGAGGCAGGTGTCTTGG
  ACCC
  
• Restriction Sites: Please inquire
• ACCN: NM_001142602
• ORF Size: 1155 bp
• Insert Size: 1800
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001142602.1, NP_001136074.1
• RefSeq Size: 1884
• RefSeq ORF: 1155
• Locus ID: 8877
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway
• Gene Summary: The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]
  Transcript Variant: This variant (4) contains an alternate in-frame segment in the 5' coding region and uses a downstream start codon, compared to variant 2. Isoform 3 has a shorter N-terminus, compared to isoform 2. Variants 3, 4 and 5 encode the same isoform (3).