Prostatic Acid Phosphatase (ACPP) (NM_001134194) Human Untagged Clone

CAT#: SC325069

ACPP (untagged)-Human acid phosphatase, prostate (ACPP), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACPP
• Synonyms: 5'-NT; ACP-3; ACP3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001134194 edited
  CAACATGAGAGCTGCACCCCTCCTCCTGGCCAGGGCAGCAAGCCTTAGCCTTGGCTTCTT
  GTTTCTGCTTTTTTTCTGGCTAGACCGAAGTGTACTAGCCAAGGAGTTGAAGTTTGTGAC
  TTTGGTGTTTCGGCATGGAGACCGAAGTCCCATTGACACCTTTCCCACTGACCCCATAAA
  GGAATCCTCATGGCCACAAGGATTTGGCCAACTCACCCAGCTGGGCATGGAGCAGCATTA
  TGAACTTGGAGAGTATATAAGAAAGAGATATAGAAAATTCTTGAATGAGTCCTATAAACA
  TGAACAGGTTTATATTCGAAGCACAGACGTTGACCGGACTTTGATGAGTGCTATGACAAA
  CCTGGCAGCCCTGTTTCCCCCAGAAGGTGTCAGCATCTGGAATCCTATCCTACTCTGGCA
  GCCCATCCCGGTGCACACAGTTCCTCTTTCTGAAGATCAGTTGCTATACCTGCCTTTCAG
  GAACTGCCCTCGTTTTCAAGAACTTGAGAGTGAGACTTTGAAATCAGAGGAATTCCAGAA
  GAGGCTGCACCCTTATAAGGATTTTATAGCTACCTTGGGAAAACTTTCAGGATTACATGG
  CCAGGACCTTTTTGGAATTTGGAGTAAAGTCTACGACCCTTTATATTGTGAGAGTGTTCA
  CAATTTCACTTTACCCTCCTGGGCCACTGAGGACACCATGACTAAGTTGAGAGAATTGTC
  AGAATTGTCCCTCCTGTCCCTCTATGGAATTCACAAGCAGAAAGAGAAATCTAGGCTCCA
  AGGGGGTGTCCTGGTCAATGAAATCCTCAATCACATGAAGAGAGCAACTCAGATACCAAG
  CTACAAAAAACTCATCATGTATTCTGCGCATGACACTACTGTGAGTGGCCTACAGATGGC
  GCTAGATGTTTACAACGGACTCCTTCCTCCCTATGCTTCTTGCCACTTGACGGAATTGTA
  CTTTGAGAAGGGGGAGTACTTTGTGGAGATGTACTATCGGAATGAGACGCAGCACGAGCC
  GTATCCCCTCATGCTACCTGGCTGCAGCCCCAGCTGTCCTCTGGAGAGGTTTGCTGAGCT
  GGTTGGCCCTGTGATCCCTCAAGACTGGTCCACGGAGTGTATGACCACAAACAGCCATCA
  AGTTCTAAAGGTCATCTTTGCTGTTGCCTTTTGCCTGATATCTGCTGTCCTAATGGTACT
  ACTGTTTATCCACATTCGCCGTGGACTCTGCTGGCAGAGAGAATCCTATGGGAACATCTG
  AACAGACAGCTGGATAAGCCAGGCCAACCTCCTGTGACACAGCATCTCTCAGTGGTGCCG
  CATCTAAAGGACAGGCTTTTGCCATGTGGGCATTGCCATCCTCACACCCTGCCTTTTGAA
  TCTGCCCAGAGCAGGGTCTGAACTCACAGTGACCCACAGACTGCTGCTGGATGAACACTC
  AGGCTACCTAGGTCTTCTCATAATGGAGTGGTAGTGCAGGCTGCTTGTTCAGCTAGAAAG
  AGGCTGTTATGCATAGGATGGTTGTATTCTCTGCCACAGTTGTCCGGAGAGAAATAAGAA
  GCCAAACTCCAATCAGCCTTTTACCTGCAAAGAGTGAAGGGTGAGAAGGTGGACAAAGAC
  ATAGATTAGAAAAATACTGGATATTGGGGGAAGTTAGACTAGAAGTATTATTATACAGGG
  TGATTCTTACGAAAGAAGACCCAATCATAAATTATAAATAAACTTTTTATTTAGCTATTA
  AAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001134194
• Insert Size: 1800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134194.1, NP_001127666.1
• RefSeq Size: 1842 bp
• RefSeq ORF: 1257 bp
• Locus ID: 55
• Cytogenetics: 3q22.1
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Riboflavin metabolism
• Gene Summary: 'This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]'
  Transcript Variant: This variant (2) differs at the 3' end compared to variant 1 and encodes a longer isoform (TM-PAP) with a distinct C-terminus compared to isoform PAP. TM-PAP is a type 1 transmembrane protein.