SYK (NM_001135052) Human Untagged Clone

CAT#: SC325233

SYK (untagged)-Human spleen tyrosine kinase (SYK), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SYK
• Synonyms: p72-Syk
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001135052 edited
  GGAGGTTACGGGGAACGACGCGCTGCTTTCATGCCCTTTCTTGTTCTACCTTCATCGGCC
  GAGGTGGACACCTGCGCAGGTGTGTGCCCTCCGGCCCCTGAAGCATGGCCAGCAGCGGCA
  TGGCTGACAGCGCCAACCACCTGCCCTTCTTTTTCGGCAACATCACCCGGGAGGAGGCAG
  AAGATTACCTGGTCCAGGGGGGCATGAGTGATGGGCTTTATTTGCTGCGCCAGAGCCGCA
  ACTACCTGGGTGGCTTCGCCCTGTCCGTGGCCCACGGGAGGAAGGCACACCACTACACCA
  TCGAGCGGGAGCTGAATGGCACCTACGCCATCGCCGGTGGCAGGACCCATGCCAGCCCCG
  CCGACCTCTGCCACTACCACTCCCAGGAGTCTGATGGCCTGGTCTGCCTCCTCAAGAAGC
  CCTTCAACCGGCCCCAAGGGGTGCAGCCCAAGACTGGGCCCTTTGAGGATTTGAAGGAAA
  ACCTCATCAGGGAATATGTGAAGCAGACATGGAACCTGCAGGGTCAGGCTCTGGAGCAGG
  CCATCATCAGTCAGAAGCCTCAGCTGGAGAAGCTGATCGCTACCACAGCCCATGAAAAAA
  TGCCTTGGTTCCATGGAAAAATCTCTCGGGAAGAATCTGAGCAAATTGTCCTGATAGGAT
  CAAAGACAAATGGAAAGTTCCTGATCCGAGCCAGAGACAACAACGGCTCCTACGCCCTGT
  GCCTGCTGCACGAAGGGAAGGTGCTGCACTATCGCATCGACAAAGACAAGACAGGGAAGC
  TCTCCATCCCCGAGGGAAAGAAGTTCGACACGCTCTGGCAGCTAGTCGAGCATTATTCTT
  ATAAAGCAGATGGTTTGTTAAGAGTTCTTACTGTCCCATGTCAAAAAATCGGCACACAGG
  GAAATGTTAATTTTGGAGGCCGTCCACAACTTCCAGGTTCCCATCCTGCGTCCTCCCCTG
  CCCAAGGGAACCGGCAAGAGAGTACTGTGTCATTCAATCCGTATGAGCCAGAACTTGCAC
  CCTGGGCTGCAGACAAAGGCCCCCAGAGAGAAGCCCTACCCATGGACACAGAGGTGTACG
  AGAGCCCCTACGCGGACCCCGAGGAGATCAGGCCCAAGGAGGTTTACCTGGACCGAAAGC
  TGCTGACGCTGGAAGACAAAGAACTGGGCTCTGGTAATTTTGGAACTGTGAAAAAGGGCT
  ACTACCAAATGAAAAAAGTTGTGAAAACCGTGGCTGTGAAAATACTGAAAAACGAGGCCA
  ATGACCCCGCTCTTAAAGATGAGTTATTAGCAGAAGCAAATGTCATGCAGCAGCTGGACA
  ACCCGTACATCGTGCGGATGATCGGGATATGCGAGGCCGAGTCCTGGATGCTGGTTATGG
  AGATGGCAGAACTTGGTCCCCTCAATAAGTATTTGCAGCAGAACAGACATGTCAAGGATA
  AGAACATCATAGAACTGGTTCATCAGGTTTCCATGGGCATGAAGTACTTGGAGGAGAGCA
  ATTTTGTGCACAGAGATCTGGCTGCAAGAAATGTGTTGCTAGTTACCCAACATTACGCCA
  AGATCAGTGATTTCGGACTTTCCAAAGCACTGCGTGCTGATGAAAACTACTACAAGGCCC
  AGACCCATGGAAAGTGGCCTGTCAAGTGGTACGCTCCGGAATGCATCAACTACTACAAGT
  TCTCCAGCAAAAGCGATGTCTGGAGCTTTGGAGTGTTGATGTGGGAAGCATTCTCCTATG
  GGCAGAAGCCATATCGAGGGATGAAAGGAAGTGAAGTCACCGCTATGTTAGAGAAAGGAG
  AGCGGATGGGGTGCCCTGCAGGGTGTCCAAGAGAGATGTACGATCTCATGAATCTGTGCT
  GGACATACGATGTGGAAAACAGGCCCGGATTCGCAGCAGTGGAACTGCGGCTGCGCAATT
  ACTACTATGACGTGGTGAACTAACCGCTCCCGCACCTGTCGGTGGCTGCCTTTGATCACA
  GGAGCAATCACAGGAAAATGTATCCAGAGGAATTGATTGTCAGCCACCTCCCTCTGCCAG
  TCGGGAGAGCCAGGCTTGGATGGAACATGCCCACAACTTGTCACCCAAAGCCTGTCCCAG
  GACTCACCCTCCACAAAGCAAAGGCAGTCCCGGGAGAAAAGACGGATGGCAGGATCCAAG
  GGGCTAGCTGGATTTGTTTGTTTTCTTGTCTGTGTGATTTTCATACAGGTTATTTTTACG
  ATCTGTTTCCAAATCCCTTTCATGTCTTTCCACTTCTCTGGGTCCCAGGGTGCATTTGTT
  ACTCATCGGGCCCAGGGACATTGCAGAGTGGCCTAGAGCACTCTCACCCCAAGCGGCCTT
  TTCCAAATGCCCAAGGATGCCTTAGCATGTGACTCCTGAAGGGAAGGCAAAGGCAGAGGA
  ATTTGGCTGCTTCTACGGCCATGAGACTGATCCCTGGCCACTGAAAAGCTTTCCTGACAA
  TAAAAATGTTTTGAGGCTTTAAAAAGAAAATCAAGTTTGACCAGTGCAGTTTCTAAGCAT
  GTAGCCAGTTAAGGAAAGAAAGAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001135052
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135052.1, NP_001128524.1
• RefSeq Size: 5012 bp
• RefSeq ORF: 1839 bp
• Locus ID: 6850
• Cytogenetics: 9q22.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity
• Gene Summary: 'This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (Syk(S)) that is shorter than isoform Syk(L). Both variants 2 and 4 encode isoform Syk(S). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.