PLS1 (NM_001145319) Human Untagged Clone

CAT#: SC325247

PLS1 (untagged)-Human plastin 1 (PLS1), transcript variant 1


  "NM_001145319" in other vectors (4)

Reconstitution Protocol

USD 760.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PLS1
Synonyms I-PLASTIN; I isoform; Plastin-1; plastin 1; plastin 1 (I isoform)
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001145319, the custom clone sequence may differ by one or more nucleotides


ATGGAAAACAGTACTACTACCATTTCTCGGGAGGAGCTTGAAGAACTACAAGAGGCATTTAATAAAATAG
ATATTGACAATAGTGGGTATGTCAGTGACTATGAACTTCAAGACCTGTTTAAGGAAGCAAGCCTTCCTCT
GCCTGGCTACAAGGTGCGCGAGATTGTGGAGAAAATTCTATCAGTTGCTGACAGCAACAAAGATGGCAAA
ATCAGTTTTGAAGAGTTTGTGTCACTAATGCAAGAATTAAAAAGCAAAGATATCAGCAAAACATTCCGAA
AAATAATTAACAAGAGGGAAGGGATTACTGCTATTGGAGGAACTTCAACTATTTCCAGTGAGGGCACACA
GCATTCTTATTCAGAGGAAGAAAAAGTGGCTTTTGTTAACTGGATAAACAAAGCCCTGGAGAATGACCCT
GACTGTAAGCATCTTATACCCATGAATCCCAATGATGATAGTCTTTTCAAGTCACTTGCAGATGGCATCC
TTCTTTGCAAAATGATCAACTTATCTGAACCAGATACAATTGATGAAAGAGCCATCAATAAGAAAAAGCT
CACGCCATTCACTATTTCTGAAAATTTAAACCTAGCTCTGAATTCTGCCTCAGCCATTGGTTGTACAGTG
GTCAACATTGGTGCATCAGATCTCAAAGAAGGAAAACCTCACTTGGTCTTGGGACTTCTCTGGCAGATCA
TCAAAGTTGGCCTTTTTGCTGATATTGAGATTTCCAGGAATGAAGCTCTGATTGCATTGTTAAATGAAGG
TGAGGAACTAGAGGAGCTGATGAAGCTTTCTCCCGAGGAATTACTGCTGCGATGGGTGAACTACCATCTG
ACCAATGCAGGATGGCATACCATCAGCAACTTCAGCCAAGACATTAAGGACTCGAGAGCCTATTTTCATC
TGCTTAATCAGATTGCCCCTAAAGGTGGGGAAGATGGACCTGCCATTGCCATTGACCTTTCAGGAATTAA
TGAGACAAATGACCTGAAGCGTGCTGGACTCATGCTTCAAGAAGCAGATAAACTGGGCTGCAAACAGTTT
GTTACTCCTGCAGATGTGGTTTCAGGCAATCCTAAACTTAATTTAGCTTTTGTAGCTAATTTGTTTAACA
CATACCCGTGCCTGCACAAGCCGAATAATAATGACATCGATATGAATTTACTGGAAGGAGAGAGCAAGGA
AGAGAGAACATTTCGGAACTGGATGAATTCCTTGGGAGTCAACCCATACATTAATCATTTGTACAGTGAC
CTTGCAGATGCTTTAGTGATCTTTCAGCTCTATGAGATGATCCGAGTGCCAGTCAACTGGAGCCATGTCA
ACAAACCTCCTTATCCTGCCCTTGGAGGGAACATGAAGAAGATTGAAAACTGTAACTATGCAGTGGAACT
TGGGAAGAACAAGGCCAAATTCTCCTTGGTTGGCATTGCTGGGCAGGACCTAAATGAAGGGAATTCAACA
CTTACCCTGGCATTGGTATGGCAGCTGATGAGAAGGTACACATTGAATGTGTTATCGGATCTTGGAGAGG
GTGAAAAAGTAAATGATGAAATTATAATTAAATGGGTCAATCAGACTCTTAAAAGTGCAAACAAAAAGAC
TTCTATTTCCAGCTTCAAGGATAAATCTATAAGCACAAGTTTACCTGTCCTAGATTTAATAGATGCCATT
GCACCAAATGCAGTTCGTCAAGAAATGATCAGGAGAGAAAACTTATCTGATGAGGACAAGCTGAACAATG
CTAAATACGCCATTTCAGTTGCTCGAAAGATCGGTGCCCGGATATATGCATTACCTGATGACCTCGTAGA
AGTGAAACCAAAGATGGTTATGACGGTGTTTGCATGCTTAATGGGAAAAGGACTGAACAGAATAAAATAA


Restriction Sites SgfI-MluI     
ACCN NM_001145319
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001145319.1, NP_001138791.1
RefSeq Size 3720 bp
RefSeq ORF 1890 bp
Locus ID 5357
Cytogenetics 3q23
Gene Summary 'Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Feb 2010]'
Transcript Variant: This variant (1) represents the more predominant transcript. Transcript variants 1, 2 and 3 encode the same protein.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.