PTPN12 (NM_001131009) Human Untagged Clone

CAT#: SC325257

PTPN12 (untagged)-Human protein tyrosine phosphatase, non-receptor type 12 (PTPN12), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPN12
• Synonyms: PTP-PEST; PTPG1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001131009, the custom clone sequence may differ by one or more nucleotides
  ATGGCCTGCCGAGAATTTGAGATGGGAAGGAAAAAATGTGAGCGCTATTGGCCTTTGTAT
  GGAGAAGACCCCATAACGTTTGCACCATTTAAAATTTCTTGTGAGGATGAACAAGCAAGA
  ACAGACTACTTCATCAGGACACTCTTACTTGAATTTCAAAATGAATCTCGTAGGCTGTAT
  CAGTTTCATTATGTGAACTGGCCAGACCATGATGTTCCTTCATCATTTGATTCTATTCTG
  GACATGATAAGCTTAATGAGGAAATATCAAGAACATGAAGATGTTCCTATTTGTATTCAT
  TGCAGTGCAGGCTGTGGAAGAACAGGTGCCATTTGTGCCATAGATTATACGTGGAATTTA
  CTAAAAGCTGGGAAAATACCAGAGGAATTTAATGTATTTAATTTAATACAAGAAATGAGA
  ACACAAAGGCATTCTGCAGTACAAACAAAGGAGCAATATGAACTTGTTCATAGAGCTATT
  GCCCAACTGTTTGAAAAACAGCTACAACTATATGAAATTCATGGAGCTCAGAAAATTGCT
  GATGGAGTGAATGAAATTAACACTGAAAACATGGTCAGCTCCATAGAGCCTGAAAAACAA
  GATTCTCCTCCTCCAAAACCACCAAGGACCCGCAGTTGCCTTGTTGAAGGGGATGCTAAA
  GAAGAAATACTGCAGCCACCGGAACCTCATCCAGTGCCACCCATCTTGACACCTTCTCCC
  CCTTCAGCTTTTCCAACAGTCACTACTGTGTGGCAGGACAATGATAGATACCATCCAAAG
  CCAGTGTTGCATATGGTTTCATCAGAACAACATTCAGCAGACCTCAACAGAAACTATAGT
  AAATCAACAGAACTTCCAGGGAAAAATGAATCAACAATTGAACAGATAGATAAAAAATTG
  GAACGAAATTTAAGTTTTGAGATTAAGAAGGTCCCTCTCCAAGAGGGACCAAAAAGTTTT
  GATGGGAACACACTTTTGAATAGGGGACATGCAATTAAAATTAAATCTGCTTCACCTTGT
  ATAGCTGATAAAATCTCTAAGCCACAGGAATTAAGTTCAGATCTAAATGTCGGTGATACT
  TCCCAGAATTCTTGTGTGGACTGCAGTGTAACACAATCAAACAAAGTTTCAGTTACTCCA
  CCAGAAGAATCCCAGAATTCAGACACACCTCCAAGGCCAGACCGCTTGCCTCTTGATGAG
  AAAGGACATGTAACGTGGTCATTTCATGGACCTGAAAATGCCATACCCATACCTGATTTA
  TCTGAAGGCAATTCCTCAGATATCAACTATCAAACTAGGAAAACTGTGAGTTTAACACCA
  AGTCCTACAACACAAGTTGAAACACCTGATCTTGTGGATCATGATAACACTTCACCACTC
  TTCAGAACACCCCTCAGTTTTACTAATCCACTTCACTCTGATGACTCAGACTCAGATGAA
  AGAAACTCTGATGGTGCTGTGACCCAGAATAAAACTAATATTTCAACAGCAAGTGCCACA
  GTTTCTGCTGCCACTAGTACTGAAAGCATTTCTACTAGGAAAGTATTGCCAATGTCCATT
  GCTAGACATAATATAGCAGGAACAACACATTCAGGTGCTGAAAAAGATGTTGATGTTAGT
  GAAGATTCACCTCCTCCCCTACCTGAAAGAACTCCTGAATCGTTTGTGTTAGCAAGTGAA
  CATAATACACCTGTAAGATCGGAATGGAGTGAACTTCAAAGTCAGGAACGATCTGAACAA
  AAAAAGTCTGAAGGCTTGATAACCTCTGAAAATGAGAAATGTGATCATCCAGCGGGAGGT
  ATTCACTATGAAATGTGCATAGAATGTCCACCTACTTTCAGTGACAAGAGAGAACAAATA
  TCAGAAAATCCAACAGAAGCCACAGATATTGGTTTTGGTAATCGATGTGGAAAACCCAAA
  GGACCAAGAGATCCACCTTCAGAATGGACA
  
• Restriction Sites: Please inquire
• ACCN: NM_001131009
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001131009.1, NP_001124481.1
• RefSeq Size: 3026 bp
• RefSeq ORF: 1953 bp
• Locus ID: 5782
• Cytogenetics: 7q11.23
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (3) contains a distinct 5' UTR, skips an internal exon, and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (3) has a shorter N-terminus with an incomplete PTPc domain when compared to isoform 1.