GUCY1A1 (NM_001130682) Human Untagged Clone

CAT#: SC325280

GUCY1A3 (untagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GUCY1A1
• Synonyms: GC-SA3; GUC1A3; GUCA3; GUCSA3; GUCY1A3; MYMY6
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001130682, the custom clone sequence may differ by one or more nucleotides
  ATGTTCTGCACGAAGCTCAAGGATCTCAAGATCACAGGAGAGTGTCCTTTCTCCTTACTG
  GCACCAGGTCAAGTTCCTAACGAGTCTTCAGAGGAGGCAGCAGGAAGCTCAGAGAGCTGC
  AAAGCAACCGTGCCCATCTGTCAAGACATTCCTGAGAAGAACATACAAGAAAGTCTTCCT
  CAAAGAAAAACCAGTCGGAGCCGAGTCTATCTTCACACTTTGGCAGAGAGTATTTGCAAA
  CTGATTTTCCCAGAGTTTGAACGGCTGAATGTTGCACTTCAGAGAACATTGGCAAAGCAC
  AAAATAAAAGAAAGCAGGAAATCTTTGGAAAGAGAAGACTTTGAAAAAACAATTGCAGAG
  CAAGCAGTTGCAGCAGGAGTTCCAGTGGAGGTTATCAAAGAATCTCTTGGTGAAGAGGTT
  TTTAAAATATGTTACGAGGAAGATGAAAACATCCTTGGGGTGGTTGGAGGCACCCTTAAA
  GATTTTTTAAACAGCTTCAGTACCCTTCTGAAACAGAGCAGCCATTGCCAAGAAGCAGGA
  AAAAGGGGCAGGCTTGAGGACGCCTCCATTCTATGCCTGGATAAGGAGGATGATTTTCTA
  CATGTTTACTACTTCTTCCCTAAGAGAACCACCTCCCTGATTCTTCCCGGCATCATAAAG
  GCAGCTGCTCACGTATTATATGAAACGGAAGTGGAAGTGTCGTTAATGCCTCCCTGCTTC
  CATAATGATTGCAGCGAGTTTGTGAATCAGCCCTACTTGTTGTACTCCGTTCACATGAAA
  AGCACCAAGCCATCCCTGTCCCCCAGCAAACCCCAGTCCTCGCTGGTGATTCCCACATCG
  CTATTCTGCAAGACATTTCCATTCCATTTCATGTTTGACAAAGATATGACAATTCTGCAA
  TTTGGCAATGGCATCAGAAGGCTGATGAACAGGAGAGACTTTCAAGGAAAGCCTAATTTT
  GAAGAATACTTTGAAATTCTGACTCCAAAAATCAACCAGACGTTTAGCGGGATCATGACT
  ATGTTGAATATGCAGTTTGTTGTACGAGTGAGGAGATGGGACAACTCTGTGAAAAAATCT
  TCAAGGGTTATGGACCTCAAAGGCCAAATGATCTACATTGTTGAATCCAGTGCAATCTTG
  TTTTTGGGGTCACCCTGTGTGGACAGATTAGAAGATTTTACAGGACGAGGGCTCTACCTC
  TCAGACATCCCAATTCACAATGCACTGAGGGATGTGGTCTTAATAGGGGAACAAGCCCGA
  GCTCAAGATGGCCTGAAGAAGAGGCTGGGGAAGCTGAAGGCTACCCTTGAGCAAGCCCAC
  CAAGCCCTGGAGGAGGAGAAGAAAAAGACAGTAGACCTTCTGTGCTCCATATTTCCCTGT
  GAGGTTGCTCAGCAGCTGTGGCAAGGGCAAGTTGTGCAAGCCAAGAAGTTCAGTAATGTC
  ACCATGCTCTTCTCAGACATCGTTGGGTTCACTGCCATCTGCTCCCAGTGCTCACCGCTG
  CAGGTCATCACCATGCTCAATGCACTGTACACTCGCTTCGACCAGCAGTGTGGAGAGCTG
  GATGTCTACAAGGTGGAGACCATTGGCGATGCCTATTGTGTAGCTGGGGGATTACACAAA
  GAGAGTGATACTCATGCTGTTCAGATAGCGCTGATGGCCCTGAAGATGATGGAGCTCTCT
  GATGAAGTTATGTCTCCCCATGGAGAACCTATCAAGATGCGAATTGGACTGCACTCTGGA
  TCAGTTTTTGCTGGCGTCGTTGGAGTTAAAATGCCCCGTTACTGTCTTTTTGGAAACAAT
  GTCACTCTGGCTAACAAATTTGAGTCCTGCAGTGTACCACGAAAAATCAATGTCAGCCCA
  ACAACTTACAGATTACTCAAAGACTGTCCTGGTTTCGTGTTTACCCCTCGATCAAGGGAG
  GAACTTCCACCAAACTTCCCTAGTGAAATCCCCGGAATCTGCCATTTTCTGGATGCTTAC
  CAACAAGGAACAAACTCAAAACCATGCTTCCAAAAGAAAGATGTGGAAGATGGCAATGCC
  AATTTTTTAGGCAAAGCATCAGGAATAGAT
  
• Restriction Sites: Please inquire
• ACCN: NM_001130682
• Insert Size: 9360 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130682.1, NP_001124154.1
• RefSeq Size: 9360 bp
• RefSeq ORF: 9360 bp
• Locus ID: 2982
• Cytogenetics: 4q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
• Gene Summary: 'Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1, and encodes isoform A. Variants 1, 2, 3, 4, and 8 all encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.