APLP2 (NM_001142276) Human Untagged Clone

CAT#: SC325316

APLP2 (untagged)-Human amyloid beta (A4) precursor-like protein 2 (APLP2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APLP2
• Synonyms: APLP-2; APPH; APPL2; CDEBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001142276, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCTGGTGGGGCTCA
  CGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGT
  TGCTGAGCCTCAAATCGCAATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGAAATGG
  GAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAGTTCTTCAGTACTGTCAGGAGA
  TGTATCCAGAGCTACAGATCACAAATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCG
  GAGGGACAAAAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTGGGTGAATTTGTAAGT
  GATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCACAAAGAGCGGATGGAGGTGTGTGAGAATCACC
  AGCACTGGCACACGGTAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTACGGCATGCT
  GCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATATGTGTGCTGCCCTCAGACAAAGATTATTGGA
  TCTGTGTCAAAAGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGACTATG
  ATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCTGTGGATGAGGA
  TGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGGAGGACCGAGATTACTACTATGACACCTTCAAAGGA
  GATGACTACAATGAGGAGAATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGACAAGGAAATTACTC
  ATGATGTCAAAGCTGTCTGCTCCCAGGAGGCGATGACGGGGCCCTGCCGGGCCGTGATGCCTCGTTGGTA
  CTTCGACCTCTCCAAGGGAAAGTGCGTGCGCTTTATATATGGTGGCTGCGGCGGCAACAGGAACAATTTT
  GAGTCTGAGGATTATTGTATGGCTGTGTGTAAAGCGATGATTCCTCCAACTCCTCTGCCAACCAATGATG
  TTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGCATGCTCGCTTCCAGAAGGCTAAGGAGCAGCT
  GGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGAGCTTCAAGCTAAG
  AACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATGGTTAAAGCTTTAGAGAAGG
  AAGCAGCCAGTGAGAAGCAGCAGCTGGTGGAGACCCACCTGGCCCGAGTGGAAGCTATGCTGAATGACCG
  CCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCCTCATCGCATTCTC
  CAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGTCATTACCAGCATG
  TGTTGGCTGTTGACCCAGAAAAGGCGGCCCAGATGAAATCCCAGGTGATGACACATCTCCACGTGATTGA
  AGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTAGCCCAAGAAATTCAAGAGGAA
  ATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTCACTGCCTCAATCTCAGAGACCCCTG
  TGGACGTCCGGGTGAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTTCCACCCCTTCCCAGC
  CCTACCTGAGAACGAAGGATCTGGAGTGGGAGAGCAGGATGGGGGACTGATCGGTGCCGAAGAGAAAGTG
  ATTAACAGTAAGAATAAAGTGGATGAAAACATGGTCATTGACGAGACTCTGGATGTTAAGGAAATGATTT
  TCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACTGCGGGAGGACTTCAG
  TCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCAGTGGCCATTGCCACGGTCATCGTCATCAGC
  CTGGTGATGCTGAGGAAGAGGCAGTATGGCACCATCAGCCACGGGATCGTGGAGGTTGATCCAATGCTCA
  CCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGCTATGAGAACCCCACCTACAAATACCTGGA
  GCAGATGCAGATTTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142276
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142276.1, NP_001135748.1
• RefSeq Size: 3791 bp
• RefSeq ORF: 2256 bp
• Locus ID: 334
• Cytogenetics: 11q24.3
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes amyloid precursor- like protein 2 (APLP2), which is a member of the APP (amyloid precursor protein) family including APP, APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 2 which lacks an internal segment in the C-terminal region, compared to isoform 1.