HMGCR (NM_001130996) Human Untagged Clone

CAT#: SC325352

HMGCR (untagged)-Human 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HMGCR
• Synonyms: LDLCQ3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC325352 sequence for NM_001130996 edited (data generated by NextGen Sequencing)
  
  
  ATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCCATCCCTGGGAAGTCATAGTGGGGA
  CAGTGACACTGACCATCTGCATGATGTCCATGAACATGTTTACTGGTAACAATAAGATCTGTGGTTGGAA
  TTATGAATGTCCAAAGTTTGAAGAGGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGC
  ATAGCCATCCTGTATATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTGGGTA
  TTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCACTTCTTAGACAAAGA
  ATTGACAGGCTTGAATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTA
  GCAAAGTTTGCCCTCAGTTCCAACTCACAGGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTT
  TAGGTCCTACGTTTACCCTCGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCAGGGGT
  ACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTACTTCGTGTTCATGACT
  TTCTTCCCAGCTTGTGTGTCCTTGGTATTAGAGCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGC
  AGCTCAGCCATTTTGCCCGAGTTTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAA
  GATGATTATGTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCAA
  AACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAGAATTGAACCAA
  GTGTTTCCCTCTGGCAGTTTTATCTCTCTAAAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAG
  TTTAGCTCTCCTTCTGGCTGTCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTA
  AAAAACCCTATCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTA
  TGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAGAAAGAAAAGT
  TGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTGGTTGGTAACTCC
  TCCTTACTCGATACTTCATCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGC
  CTAATGAAGAATGTCTACAGATACTTGGGAATGCAGAGAAAGGTGCAAAATTCCTTAGTGATGCTGAGAT
  CATCCAGTTAGTCAATGCTAAGCATATCCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGT
  GGTGTATCTATTCGCCGACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTT
  ACAGGGATTATAATTACTCCTTGCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCG
  TGGCCCAGTTGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAA
  GGGTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCAGATTTGCACGTCTACAGAAACTTCATACAA
  GTATAGCTGGACGCAACCTTTATATCCGTTTCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGAT
  TTCAAAGGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGCCGTT
  AGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAGAGGAAAATCTGTTG
  TTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGAAGTATTAAAGACTACCACAGAGGCTATGATTGA
  GGTCAACATTAACAAGAATTTAGTGGGCTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCA
  GCAAACATTGTCACCGCCATCTACATTGCCTGTGGACAGGATGCAGCACAGAATGTTGGTAGTTCAAACT
  GTATTACTTTAATGGAAGCAAGTGGTCCCACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTAT
  AGAGATAGGAACGGTGGGTGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCAGATGCTAGGTGTT
  CAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGCCCGAATTGTGTGTGGGACCGTAA
  TGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGCAGGACATCTTGTCAAAAGTCACATGATTCACAA
  CAGGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGA
  
  Clone variation with respect to NM_001130996.1
  
• Restriction Sites: Please inquire
• ACCN: NM_001130996
• ORF Size: 2508 bp
• Insert Size: 3800
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001130996.1.

Reference Data

• RefSeq: NM_001130996.1, NP_001124468.1
• RefSeq Size: 4430
• RefSeq ORF: 2508
• Locus ID: 3156
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Terpenoid backbone biosynthesis
• Gene Summary: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
  Transcript Variant: This variant (2) lacks an in-frame coding exon in the middle region, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1.