PRMT3 (NM_001145166) Human Untagged Clone

CAT#: SC325968

PRMT3 (untagged)-Human protein arginine methyltransferase 3 (PRMT3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRMT3
• Synonyms: HRMT1L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145166, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGCTCGTTAGCGTCAGGCGCTACCGGTTATTCACATCTGCTGAAGAAACATTTTCACACTGTAAGTC
  TGAGCATCAGTTTAATATTGACAGCATGGTTCATAAACATGAATCCTACAGTTGAGTACATGAATTCCAT
  ATACAACCCAGTGCCTTGGGAGAAAGAAGAGTATTTGAAGCCAGTATTAGAAGATGACCTTTTACTTCAA
  TTTGATGTAGAAGATCTTTATGAACCGGTGTCAGTACCCTTCTCATACCCCAATGGACTCAGTGAAAATA
  CATCTGTTGTTGAAAAATTGAAACATATGGAAGCCAGGGCACTGTCTGCTGAAGCCGCATTGGCCAGAGC
  ACGTGAGGATCTGCAAAAAATGAAACAATTTGCTCAGGATTTTGTGATGCACACAGATGTCAGAACCTGC
  TCGTCATCTACTAGTGTCATTGCGGACCTCCAGGAGGATGAGGATGGTGTTTATTTCAGCTCATACGGGC
  ATTATGGGATACATGAAGAAATGCTAAAGGACAAAATACGAACAGAAAGCTACCGAGATTTCATATACCA
  AAATCCACATATCTTCAAAGACAAGGTAGTTTTGGATGTTGGGTGTGGAACTGGAATTCTCTCTATGTTT
  GCTGCTAAAGCTGGGGCGAAGAAGGTTCTTGGAGTTGATCAATCTGAAATACTTTACCAGGCAATGGATA
  TTATAAGACTAAATAAACTTGAAGATACTATTACACTAATTAAAGGAAAGATTGAAGAAGTTCATCTTCC
  TGTAGAAAAAGTAGATGTTATCATATCTGAGTGGATGGGCTATTTTCTTCTGTTTGAGTCTATGTTAGAT
  TCTGTCCTTTATGCAAAGAACAAATACTTGGCAAAAGGAGGCTCGGTCTACCCTGACATTTGCACTATCA
  GCCTTGTAGCAGTGAGTGATGTGAATAAACATGCTGATAGAATTGCTTTTTGGGATGATGTCTATGGCTT
  CAAGATGTCCTGCATGAAGAAAGCAGTTATTCCAGAAGCTGTTGTGGAAGTTTTAGATCCGAAGACTCTT
  ATTTCAGAACCTTGTGGTATTAAGCATATAGATTGCCATACGACGTCTATCTCAGATTTGGAATTTTCAT
  CAGATTTTACCCTGAAAATCACAAGGACATCCATGTGCACGGCAATTGCTGGCTACTTTGATATATATTT
  TGAGAAGAATTGCCACAACAGGGTCGTGTTCTCTACGGGCCCTCAGAGCACCAAAACACACTGGAAACAA
  ACAGTATTTCTACTGGAAAAACCATTTTCAGTTAAAGCAGGTGAAGCCTTGAAAGGAAAGGTCACAGTTC
  ACAAGAATAAGAAAGATCCACGTTCTCTCACCGTGACCCTCACGTTGAATAATTCAACTCAAACTTATGG
  TCTCCAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145166
• ORF Size: 1410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001145166.1, NP_001138638.1
• RefSeq Size: 2557
• RefSeq ORF: 1410
• Locus ID: 10196
• Protein Families: Druggable Genome
• Gene Summary: This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (2) is missing two non-consecutive internal coding exons compared to transcript variant 1. It uses the same translation start site as variant 1, however, the first missing exon throws it out of frame, and the second missing exon puts it back in frame, resulting in a shorter isoform (2) with a novel 28 aa segment compared to isoform 1.