GLB1 (NM_001135602) Human Untagged Clone

CAT#: SC326059

GLB1 (untagged)-Human galactosidase, beta 1 (GLB1), transcript variant 3


  "NM_001135602" in other vectors (4)

Reconstitution Protocol

USD 920.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GLB1
Synonyms EBP; ELNR1; MPS4B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001135602 edited
CCCTCGTGCCGAATTCGGCACGAGGGTCAAGTGACGCGAAGCGGCCGGCCTGGGCGCCGA
CTGCAGAGCCGGGAGGCTGGTGGTCATGCCGGGGTTCCTGGTTCGCATCCTCCCTCTGCT
GCTGGTTCTGCTGCTTCTGGGCCCTACGCGCGGCTTGCGCAATGCCACCCAGAGGATGTT
TGAAATTGACTATAGCCGGGACTCCTTCCTCAAGGATGGCCAGCCATTTCGCTACATCTC
AGGAAGCATTCACTACTCCCGTGTGCCCCGCTTCTACTGGAAGGACCGGCTGCTGAAGAT
GAAGATGGCTGGGCTGAACGCCATCCAGACATTACCTGGCAGCTGTGGACAAGTGGTTGG
GAGTCCTTCTGCCCAAGATGAAGCCTCTCCTCTATCAGAATGGAGGGCCAGTTATAACAG
TGCAGGCAGCAACATCACAGATGCTTTCCTAAGCCAGAGGAAGTGTGAGCCCAAAGGACC
CTTGATCAATTCTGAATTCTATACTGGCTGGCTAGATCACTGGGGCCAACCTCACTCCAC
AATCAAGACCGAAGCAGTGGCTTCCTCCCTCTATGATATACTTGCCCGTGGGGCGAGTGT
GAACTTGTACATGTTTATAGGTGGGACCAATTTTGCCTATTGGAATGGGGCCAACTCACC
CTATGCAGCACAGCCCACCAGCTACGACTATGATGCCCCACTGAGTGAGGCTGGGGACCT
CACTGAGAAGTATTTTGCTCTGCGAAACATCATCCAGAAGTTTGAAAAAGTACCAGAAGG
TCCTATCCCTCCATCTACACCAAAGTTTGCATATGGAAAGGTCACTTTGGAAAAGTTAAA
GACAGTGGGAGCAGCTCTGGACATTCTGTGTCCCTCTGGGCCCATCAAAAGCCTTTATCC
CTTGACATTTATCCAGGTGAAACAGCATTATGGGTTTGTGCTGTACCGGACAACACTTCC
TCAAGATTGCAGCAACCCAGCACCTCTCTCTTCACCCCTCAATGGAGTCCACGATCGAGC
ATATGTTGCTGTGGATGGGATCCCCCAGGGAGTCCTTGAGCGAAACAATGTGATCACTCT
GAACATAACAGGGAAAGCTGGAGCCACTCTGGACCTTCTGGTAGAGAACATGGGACGTGT
GAACTATGGTGCATATATCAACGATTTTAAGGGTTTGGTTTCTAACCTGACTCTCAGTTC
CAATATCCTCACGGACTGGACGATCTTTCCACTGGACACTGAGGATGCAGTGCGCAGCCA
CCTGGGGGGCTGGGGACACCGTGACAGTGGCCACCATGATGAAGCCTGGGCCCACAACTC
ATCCAACTACACGCTCCCGGCCTTTTATATGGGGAACTTCTCCATTCCCAGTGGGATCCC
AGACTTGCCCCAGGACACCTTTATCCAGTTTCCTGGATGGACCAAGGGCCAGGTCTGGAT
TAATGGCTTTAACCTTGGCCGCTATTGGCCAGCCCGGGGCCCTCAGTTGACCTTGTTTGT
GCCCCAGCACATCCTGATGACCTCGGCCCCAAACACCATCACCGTGCTGGAACTGGAGTG
GGCACCCTGCAGCAGTGATGATCCAGAACTATGTGCTGTGACGTTCGTGGACAGGCCAGT
TATTGGCTCATCTGTGACCTACGATCATCCCTCCAAACCTGTTGAAAAAAGACTCATGCC
CCCACCCCCGCAAAAAAACAAAGATTCATGGCTGGACCATGTATGATGATGAAAGCCTGT
GTCTTTGAGGGATTCTACCCTGAACATACCTCACAGATCCTCCCTGTCATGCCACATTTC
ACTGATTGGAATGTGGAAATGGAAAAGGAATTTAGGATGTGCATTTTCACCTGAGGTTTC
CCTGCATCCCTGCAGTGCCAAAGCCCCACCTTCAGGGACCACCTGGAATGTGTGAGGGGC
TGACAGCACAGTAACGTGCATACATATCTGCAGGGCTGGAATGGAAGCTTTAAAGGTGGT
AGTGATTTTTATTTTGGAAGAATCATGTTACCTTTTTGTTAAATAAAATTTGTACTCAAA
AAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_001135602
Insert Size 2100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001135602.1, NP_001129074.1
RefSeq Size 2195 bp
RefSeq ORF 1641 bp
Locus ID 2720
Cytogenetics 3p22.3
Protein Families Druggable Genome
Protein Pathways Galactose metabolism, Glycosaminoglycan degradation, Glycosphingolipid biosynthesis - ganglio series, Lysosome, Metabolic pathways, Other glycan degradation, Sphingolipid metabolism
Gene Summary 'This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome. [provided by RefSeq, Nov 2015]'
Transcript Variant: This variant (3) lacks three alternate exons in the coding region compared to variant 1. The encoded isoform (c) is shorter than isoform a. This isoform (c) may undergo proteolytic processing similar to isoform a.

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