SnoN (SKIL) (NM_001145097) Human Untagged Clone

CAT#: SC326131

SKIL (untagged)-Human SKI-like oncogene (SKIL), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SKIL
• Synonyms: SNO; SnoA; SnoI; SnoN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145097, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAAACCTCCAGACAAATTTCTCCTTGGTTCAGGGCTCAACTAAAAAACTGAATGGGATGGGAGATG
  ATGGCAGCCCCCCAGCGAAAAAAATGATAACGGACATTCATGCAAATGGAAAAACGATAAACAAGGTGCC
  AACAGTTAAGAAGGAACACTTGGATGACTATGGAGAAGCACCAGTGGAAACTGATGGAGAGCATGTTAAG
  CGAACCTGTACTTCTGTTCCTGAAACTTTGCATTTAAATCCCAGTTTGAAACACACATTGGCACAATTCC
  ATTTAAGTAGTCAGAGCTCGCTGGGTGGACCAGCAGCATTTTCTGCTCGGCATTCCCAAGAAAGCATGTC
  GCCTACTGTATTTCTGCCTCTTCCATCACCTCAGGTTCTTCCTGGCCCATTGCTCATCCCTTCAGATAGC
  TCCACAGAACTCACTCAGACTGTGTTGGAAGGGGAATCTATTTCTTGTTTTCAAGTTGGAGGAGAAAAGA
  GACTCTGTTTGCCCCAAGTCTTAAATTCTGTTCTCCGAGAATTTACACTCCAGCAAATAAATACAGTGTG
  TGATGAACTGTACATATATTGTTCAAGGTGTACTTCAGACCAGCTTCATATCTTAAAGGTACTGGGCATA
  CTTCCATTCAATGCCCCATCCTGTGGGCTGATTACATTAACTGATGCACAAAGATTATGTAATGCTTTAT
  TGCGGCCACGAACTTTTCCTCAAAATGGTAGCGTACTTCCTGCTAAAAGCTCATTGGCCCAGTTAAAGGA
  AACTGGCAGTGCCTTTGAAGTGGAGCATGAATGCCTAGGCAAATGTCAGGGTTTATTTGCACCCCAGTTT
  TATGTTCAGCCTGATGCTCCGTGTATTCAATGTCTGGAGTGTTGTGGAATGTTTGCACCCCAGACGTTTG
  TGATGCATTCTCACAGATCACCTGACAAAAGAACTTGCCACTGGGGCTTTGAATCAGCTAAATGGCATTG
  CTATCTTCATGTGAACCAAAAATACTTAGGAACACCTGAAGAAAAGAAACTGAAGATAATTTTAGAAGAA
  ATGAAGGAGAAGTTTAGCATGAGAAGTGGAAAGAGAAATCAATCCAAGACAGATGCACCATCAGGAATGG
  AATTACAGTCATGGTATCCTGTTATAAAGCAGGAAGGTGACCATGTTTCTCAGACACATTCATTTTTACA
  CCCCAGCTACTACTTATACATGTGTGATAAAGTGGTTGCCCCAAATGTGTCACTTACTTCTGCTGTATCC
  CAGTCTAAAGAGCTCACAAAGACAGAGGCAAATGCATCAATCTCAAATAATTCTACAAGTAAAAGGAAAT
  CTGAGTCTGCCACTTGCAACTTAGTCAGAGACATAAACAAAGTGGGAATTGGCCTTGTTGCTGCCGCTTC
  ATCTCCGCTTCTTGTGAAAGATGTCATTTGTGAGGATGATAAGGGAAAAATCATGGAAGAAGTAATGAGA
  ACTTATTTAAAACAACAGGAAAAACTAAACTTGATTTTGCAAAAGAAGCAACAACTTCAGATGGAAGTAA
  AAATGTTGAGTAGTTCAAAATCTATGAAGGAACTCACTGAAGAACAGCAGAATTTACAGAAAGAGCTTGA
  ATCTTTGCAGAATGAACATGCTCAAAGAATGGAAGAATTTTATGTTGAACAGAAAGACTTAGAGAAAAAA
  TTGGAGCAGATAATGAAGCAAAAATGTACCTGTGACTCAAATTTAGAAAAAGACAAAGAGGCTGAATATG
  CAGGACAGTTGGCAGAACTGAGGCAGAGATTGGACCATGCTGAGGCCGATAGGCAAGAACTCCAAGATGA
  ACTCAGACAGGAACGGGAAGCAAGACAGAAGTTAGAGATGATGATAAAAGAGCTAAAGCTGCAAATTCTG
  AAATCATCAAAGACTGCTAAAGAATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145097
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145097.2, NP_001138569.1
• RefSeq Size: 7061 bp
• RefSeq ORF: 1917 bp
• Locus ID: 6498
• Cytogenetics: 3q26.2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (2, also known as SnoN2) differs in the 5' UTR and uses an alternate in-frame splice site in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.