Choline Acetyltransferase (CHAT) (NM_001142933) Human Untagged Clone

CAT#: SC326156

CHAT (untagged)-Human choline O-acetyltransferase (CHAT), transcript variant S

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHAT
• Synonyms: CHOACTASE; CMS1A; CMS1A2; CMS6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001142933, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGCCGGAATGCAGAGATGAAGCACTGAGCACAGTAGGTCCACACCTCTGCATCCCTGCACCAGGAC
  TCACCAAGACGCCCATCCTGGAAAAGGTCCCCCGTAAGATGGCAGCAAAAACTCCCAGCAGTGAGGAGTC
  TGGGCTGCCCAAACTGCCCGTGCCCCCGCTGCAGCAGACCCTGGCCACGTACCTGCAGTGCATGCGACAC
  TTGGTGTCTGAGGAGCAGTTCAGGAAGAGCCAGGCCATTGTGCAGCAGTTTGGGGCCCCTGGTGGCCTCG
  GCGAGACCCTGCAGCAGAAACTCCTGGAGCGGCAGGAGAAGACAGCCAACTGGGTGTCTGAGTACTGGCT
  GAATGACATGTATCTCAACAACCGCCTGGCCCTGCCTGTCAACTCCAGCCCTGCCGTGATCTTTGCTCGG
  CAGCACTTCCCTGGCACCGATGACCAGCTGAGGTTTGCAGCCAGCCTCATCTCTGGTGTACTCAGCTACA
  AGGCCCTGCTGGACAGCCACTCCATTCCCACTGACTGTGCCAAAGGCCAGCTGTCAGGGCAGCCCCTTTG
  CATGAAGCAATACTATGGGCTCTTCTCCTCCTACCGGCTCCCCGGCCATACCCAGGACACGCTGGTGGCT
  CAGAACAGCAGCATCATGCCGGAGCCTGAGCACGTCATCGTAGCCTGCTGCAATCAGTTCTTTGTCTTGG
  ATGTTGTCATTAATTTCCGCCGTCTCAGTGAGGGGGATCTGTTCACTCAGTTGAGAAAGATAGTCAAAAT
  GGCTTCCAACGAGGACGAGCGTTTGCCTCCAATTGGCCTGCTGACGTCTGACGGGAGGAGCGAGTGGGCC
  GAGGCCAGGACGGTCCTCGTGAAAGACTCCACCAACCGGGACTCGCTGGACATGATTGAGCGCTGCATCT
  GCCTTGTATGCCTGGACGCGCCAGGAGGCGTGGAGCTCAGCGACACCCACAGGGCACTCCAGCTCCTTCA
  CGGCGGAGGCTACAGCAAGAACGGGGCCAATCGCTGGTACGACAAGTCCCTGCAGTTTGTGGTGGGCCGA
  GACGGCACCTGCGGTGTGGTGTGCGAACACTCCCCATTCGATGGCATCGTCCTGGTGCAGTGCACTGAGC
  ATCTGCTCAAGCACATGACGCAGAGCAGCAGGAAGCTGATCCGAGCAGACTCCGTCAGCGAGCTCCCCGC
  CCCCCGGAGGCTGCGGTGGAAATGCTCCCCGGAAATTCAAGGCCACTTAGCCTCCTCGGCAGAAAAACTT
  CAACGAATAGTAAAGAACCTTGACTTCATTGTCTATAAGTTTGACAACTATGGGAAAACATTCATTAAGA
  AGCAGAAATGCAGCCCTGATGCCTTCATCCAGGTGGCCCTCCAGCTGGCCTTCTACAGGCTCCATCGAAG
  ACTGGTGCCCACCTACGAGAGCGCGTCCATCCGCCGATTCCAGGAGGGACGCGTGGACAACATCAGATCG
  GCCACTCCAGAGGCACTGGCTTTTGTGAGAGCCGTGACTGACCACAAGGCTGCTGTGCCAGCTTCTGAGA
  AGCTTCTGCTCCTGAAGGATGCCATCCGTGCCCAGACTGCATACACAGTCATGGCCATAACAGGGATGGC
  CATTGACAACCACCTGCTGGCACTGCGGGAGCTGGCCCGGGCCATGTGCAAGGAGCTGCCCGAGATGTTC
  ATGGATGAAACCTACCTGATGAGCAACCGGTTTGTCCTCTCCACTAGCCAGGTGCCCACAACCACGGAGA
  TGTTCTGCTGCTATGGTCCTGTGGTCCCAAATGGGTATGGTGCCTGCTACAACCCCCAGCCAGAGACCAT
  CCTTTTCTGCATCTCTAGCTTTCACAGCTGCAAAGAGACTTCTTCTAGCAAGTTTGCAAAAGCTGTGGAA
  GAAAGCCTCATTGACATGAGAGACCTCTGCAGTCTGCTGCCGCCTACTGAGAGCAAGCCATTGGCAACAA
  AGGAAAAAGCCACGAGGCCCAGCCAGGGACACCAACCTTGA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001142933
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142933.1, NP_001136405.1
• RefSeq Size: 2528 bp
• RefSeq ORF: 2001 bp
• Locus ID: 1103
• Cytogenetics: 10q11.23
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism
• Gene Summary: 'This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (S) contains alternate 5' exons M and S in the 5' coding region, and uses an upstream start codon (PMID 11172068). The resulting protein (isoform 3) has a longer N-terminus compared to isoform 1.