ASAH2 (NM_001143974) Human Untagged Clone

CAT#: SC326211

ASAH2 (untagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASAH2
• Synonyms: BCDase; HNAC1; LCDase; N-CDase; NCDase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001143974, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAAACGCACCTTCTCTAACTTGGAGACATTCCTGATTTTCCTCCTTGTAATGATGAGTGCCATCA
  CAGTGGCCCTTCTCAGCCTCTTGTTTATCACCAGTGGGACCATTGAAAACCACAAAGATTTAGGAGGCCA
  TTTTTTTTCAACCACCCAAAGCCCTCCAGCCACCCAGGGCTCCACAGCTGCCCAACGCTCCACAGCCACC
  CAGCATTCCACAGCCACCCAGAGCTCCACAGCCACTCAAACTTCTCCAGTGCCTTTAACCCCAGAGTCTC
  CTCTATTTCAGAACTTCAGTGGCTACCATATTGGTGTTGGACGAGCTGACTGCACAGGACAAGTAGCAGA
  TATCAATTTGATGGGCTATGGCAAATCCGGCCAGAATGCACAGGGCATCCTCACCAGGCTATACAGTCGT
  GCCTTCATCATGGCAGAACCTGATGGGTCCAATCGAACAGTGTTTGTCAGCATCGACATAGGCATGGTAT
  CACAAAGGCTCAGGCTGGAGGTCCTGAACAGACTGCAGAGTAAATATGGCTCCCTGTACAGAAGAGATAA
  TGTCATCCTGAGTGGCACTCACACTCATTCAGGTCCTGCAGGATATTTCCAGTATACCGTGTTTGTAATT
  GCCAGTGAAGGATTTAGCAATCAAACTTTTCAGCACATGGTCACTGGTATCTTGAAGAGCATTGACATAG
  CACACACAAATATGAAACCAGGCAAAATCTTCATCAATAAAGGAAATGTGGATGGTGTGCAGATCAACAG
  AAGTCCGTATTCTTACCTTCAAAATCCGCAGTCAGAGAGAGCAAGGTATTCTTCAAATACAGACAAGGAA
  ATGATAGTTTTGAAAATGGTAGATTTGAATGGAGATGACTTGGGCCTTATCAGCTGGTTTGCCATCCACC
  CGGTCAGCATGAACAACAGTAACCATCTTGTAAACAGTGACAATGTGGGCTATGCATCTTACCTGCTTGA
  GCAAGAGAAGAACAAAGGATATCTACCTGGACAGGGGCCATTTGTAGCAGCCTTTGCTTCATCAAACCTA
  GGAGATGTGTCCCCCAACATTCTTGGACCACGTTGCATCAACACAGGAGAGTCCTGTGATAACGCCAATA
  GCACTTGTCCCATTGGTGGGCCTAGCATGTGCATTGCTAAGGGACCTGGACAGGATATGTTTGACAGCAC
  ACAAATTATAGGACGGGCCATGTATCAGAGAGCAAAGTCAAAAACATGTAAACCAGCATTGGGCTACAGT
  TTTGCAGCTGGCACTATTGATGGAGTTGGAGGCCTCAATTTTACACAGGGGAAAACAGAAGGGGATCCAT
  TTTGGGACACCATTCGGGACCAGATCCTGGGAAAGCCATCTGAAGAAATTAAAGAATGTCATAAACCAAA
  GCCCATCCTTCTTCACACCGGAGAACTATCAAAACCTCACCCCTGGCATCCAGACATTGTTGATGTTCAG
  ATTATTACCCTTGGGTCCTTGGCCATAACTGCCATCCCCGGGGAGTTTACGACCATGTCTGGACGAAGAC
  TTCGAGAGGCAGTTCAAGCAGAATTTGCATCTCATGGGATGCAGAACATGACTGTTGTTATTTCAGGTCT
  ATGCAACGTCTATACACATTACATTACCACTTATGAAGAATACCAGGCTCAGCGATATGAGGCAGCATCG
  ACAATTTATGGACCGCACACATTATCTGCTTACATTCAGCTCTTCAGAAACCTTGCTAAGGCTATTGCTA
  CGGACACGGTAGCCAACCTGAGCAGAGGTCCAGAACCTCCCTTTTTCAAACAATTAATAGTTCCATTAAT
  TCCTAGTATTGTGGATAGAGCACCAAAAGGCAGAACTTTCGGGGATGTCCTGCAGCCAGCAAAACCTGAA
  TACAGAGTGGGGGAAGTTGCTGAAGTTATATTTGTAGGTGCTAACCCGAAGAATTCAGTACAAAACCAGA
  CCCATCAGACCTTCCTCACTGTGGAGAAATATGAGGCTACTTCAACATCGTGGCAGATAGTGTGTAATGA
  TGCCTCCTGGGAGACTCGTTTTTATTGGCACAAGGGACTCCTGGGTCTGAGTAATGCAACAGTGGAATGG
  CATATTCCAGACACTGCCCAGCCTGGAATCTACAGAATAAGATATTTTGGACACAATCGGAAGCAGGACA
  TTCTGAAGCCTGCTGTCATACTTTCATTTGAAGGCACTTCCCCGGCTTTTGAAGTTGTAACTATTTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001143974
• ORF Size: 2238 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001143974.1, NP_001137446.1
• RefSeq Size: 2327
• RefSeq ORF: 2238
• Locus ID: 56624
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Gene Summary: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the internal coding region, compared to variant 1. The resulting isoform (b) is shorter, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.