TRPC4 (NM_001135955) Human Untagged Clone

CAT#: SC326275

TRPC4 (untagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant beta

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPC4
• Synonyms: HTRP-4; HTRP4; TRP4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001135955 edited
  TGCTCCAAAACTCAGCAGCGACTAAGGGAATTCCATTGGAATTTGCCGGGCGTGCTCTCA
  CCCCGCACGGCACCCGCGCCGTCAGTCCTCGGATCCCATCACTTCAGCCCGAAGATTGCA
  ACTTTGCAGAGACGAAGAAATAGCATGGCATGAAACATGGCTCAGTTCTATTACAAAAGA
  AATGTTAATGCTCCCTATAGAGACCGCATCCCTCTAAGGATAGTAAGAGCAGAATCAGAA
  CTCTCGCCATCAGAAAAAGCCTACTTGAATGCTGTGGAAAAGGGAGATTATGCCAGTGTC
  AAGAAATCCCTAGAGGAAGCTGAAATTTATTTTAAAATCAATATTAATTGCATTGATCCT
  CTCGGAAGAACTGCTCTCCTCATTGCAATTGAAAATGAGAACTTGGAGCTCATCGAACTA
  CTCTTAAGCTTTAATGTCTATGTTGGAGATGCTCTATTACATGCTATCAGAAAAGAAGTC
  GTCGGAGCTGTTGAGCTGTTATTGAACCACAAAAAACCTAGTGGAGAAAAACAGGTGCCT
  CCTATACTCCTTGATAAGCAGTTCTCTGAATTCACTCCAGACATTACACCAATCATTTTG
  GCAGCCCATACAAATAATTATGAGATAATAAAACTCTTGGTTCAGAAAGGAGTCTCAGTG
  CCTCGACCCCACGAGGTCCGCTGTAACTGTGTGGAATGCGTGTCCAGTTCAGATGTGGAC
  AGCCTCCGTCACTCACGCTCCAGACTCAACATCTACAAGGCCTTGGCCAGTCCCTCTCTC
  ATTGCACTGTCAAGCGAAGATCCTTTTCTCACAGCCTTTCAGTTAAGTTGGGAACTTCAG
  GAACTGAGCAAGGTGGAAAATGAATTCAAGTCGGAGTATGAAGAGCTGTCACGGCAGTGC
  AAACAATTTGCTAAGGACCTACTGGATCAGACGAGAAGTTCCAGAGAACTGGAAATCATT
  CTTAATTACCGAGATGACAATAGTCTCATAGAAGAACAAAGTGGAAATGATCTTGCAAGA
  CTAAAATTGGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCCCAGCCCAATTGTCAACAG
  CTGCTGGCATCTCGCTGGTACGATGAGTTTCCAGGCTGGAGGAGAAGACACTGGGCAGTG
  AAGATGGTGACATGTTTCATAATAGGACTTCTTTTTCCTGTCTTCTCTGTGTGCTACCTG
  ATAGCTCCCAAAAGCCCACTTGGACTGTTCATCAGGAAGCCATTTATCAAGTTTATCTGC
  CACACAGCCTCCTATTTGACTTTTTTGTTCCTGCTGCTGCTTGCCTCTCAGCACATCGAC
  AGGTCAGACTTGAACAGGCAAGGTCCACCACCAACCATCGTCGAGTGGATGATATTACCG
  TGGGTCCTGGGCTTCATATGGGGAGAAATTAAACAGATGTGGGATGGCGGACTTCAGGAC
  TACATCCATGATTGGTGGAATCTAATGGACTTTGTAATGAACTCCTTATATTTAGCAACA
  ATCTCCTTGAAAATTGTTGCATTTGTAAAGTACAGTGCCCTTAATCCACGAGAATCATGG
  GACATGTGGCATCCCACTCTGGTGGCAGAGGCTTTATTTGCTATTGCAAACATCTTCAGT
  TCTCTGCGTCTGATCTCACTGTTTACTGCAAATTCTCACCTGGGACCTCTGCAAATATCT
  CTGGGAAGAATGCTCCTGGACATTTTGAAGTTTCTATTCATATACTGCCTTGTGTTGCTA
  GCATTTGCAAATGGCCTAAATCAATTGTACTTCTATTATGAAGAAACGAAAGGGTTAACC
  TGCAAAGGCATAAGATGTGAAAAGCAGAATAATGCATTTTCAACGTTATTTGAGACACTG
  CAGTCCCTGTTTTGGTCAATATTTGGGCTCATCAATTTATATGTGACCAATGTCAAAGCA
  CAGCATGAATTTACTGAGTTTGTTGGTGCCACCATGTTTGGGACATACAATGTCATCTCT
  CTGGTTGTTCTACTCAACATGTTAATAGCTATGATGAATAATTCTTACCAACTGATTGCT
  GACCATGCAGATATAGAATGGAAATTTGCACGAACAAAGCTTTGGATGAGTTATTTTGAA
  GAAGGAGGTACTCTGCCTACTCCCTTCAATGTCATCCCGAGCCCCAAGTCTCTCTGGTAC
  CTGATCAAATGGATCTGGACACACTTGTGCAAGAAAAAGATGAGAAGAAAGCCAGAAAGT
  TTTGGAACAATAGGGAGGCGAGCTGCTGATAACTTGAGAAGACATCACCAATACCAAGAA
  GTTATGAGGAACCTGGTGAAGCGATACGTTGCTGCAATGATTAGAGATGCTAAAACTGAA
  GAAGGCCTGACCGAAGAGAACTTTAAGGAACTAAAGCAAGACATTTCTAGTTTCCGCTTT
  GAAGTCCTGGGATTACTAAGAGGAAGCAAACTTTCCACAATACAATCTGCGAATGCCTCG
  AAGGAGTCTTCAAATTCGGCAGACTCAGATGAAAAGAGTGATAGCGAAGAAGAAGTTGCT
  CGTCAACAGGCTGCAGGACCACTTGAGAGAAATATTCAACTGGAATCTCGAGGATTAGCT
  TCACGGGGTGACCTGAGCATTCCCGGTCTCAGTGAACAATGTGTGTTAGTAGACCATAGA
  GAAAGGAATACGGACACACTGGGGTTACAGGTAGGAAAGAGAGTGTGTCCATTCAAGTCA
  GAGAAGGTGGTGGTGGAGGACACGGTTCCTATAATACCAAAGGAGAAACATGCAAAAGAA
  GAGGACTCTAGTATAGACTATGATCTAAACCTCCCAGACACAGTCACCCACGAAGATTAC
  GTGACCACAAGATTGTGATACTTGAAGGAGGAAGCGTTTACCATACACATACGTATTTTC
  CGTAGTGCTCTGGGTGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_001135955
• Insert Size: 2900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001135955.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135955.1, NP_001129427.1
• RefSeq Size: 3282 bp
• RefSeq ORF: 2682 bp
• Locus ID: 7223
• Cytogenetics: 13q13.3
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: 'This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (2, also known as beta) lacks an in-frame segment in the 3' coding region, compared to variant 1 (alpha). The encoded isoform (beta) is shorter, compared to isoform alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.