NFAT1 (NFATC2) (NM_001136021) Human Untagged Clone

CAT#: SC326282

NFATC2 (untagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 (NFATC2), transcript variant D

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFATC2
• Synonyms: NFAT1; NFATP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001136021, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGAGAGAGGCTGCGTTCAGACTGGGGCACTGCCATCCCCTCCGCATCATGGGGTCTGTGGACCAAG
  AAGAGCCGAATGCACATAAGGTCGCCAGCCCACCCTCCGGACCCGCATACCCCGATGATGTCCTGGACTA
  TGGCCTCAAGCCATACAGCCCCCTTGCTAGTCTCTCTGGCGAGCCCCCCGGCCGATTCGGAGAGCCGGAT
  AGGGTAGGGCCGCAGAAGTTTCTGAGCGCGGCCAAGCCAGCAGGGGCCTCGGGCCTGAGCCCTCGGATCG
  AGATCACTCCGTCCCACGAACTGATCCAGGCAGTGGGGCCCCTCCGCATGAGAGACGCGGGCCTCCTGGT
  GGAGCAGCCGCCCCTGGCCGGGGTGGCCGCCAGCCCGAGGTTCACCCTGCCCGTGCCCGGCTTCGAGGGC
  TACCGCGAGCCGCTTTGCTTGAGCCCCGCTAGCAGCGGCTCCTCTGCCAGCTTCATTTCTGACACCTTCT
  CCCCCTACACCTCGCCCTGCGTCTCGCCCAATAACGGCGGGCCCGACGACCTGTGTCCGCAGTTTCAAAA
  CATCCCTGCTCATTATTCCCCCAGAACCTCGCCAATAATGTCACCTCGAACCAGCCTCGCCGAGGACAGC
  TGCCTGGGCCGCCACTCGCCCGTGCCCCGTCCGGCCTCCCGCTCCTCATCGCCTGGTGCCAAGCGGAGGC
  ATTCGTGCGCCGAGGCCTTGGTTGCCCTGCCGCCCGGAGCCTCACCCCAGCGCTCCCGGAGCCCCTCGCC
  GCAGCCCTCATCTCACGTGGCACCCCAGGACCACGGCTCCCCGGCTGGGTACCCCCCTGTGGCTGGCTCT
  GCCGTGATCATGGATGCCCTGAACAGCCTCGCCACGGACTCGCCTTGTGGGATCCCCCCCAAGATGTGGA
  AGACCAGCCCTGACCCCTCGCCGGTGTCTGCCGCCCCATCCAAGGCCGGCCTGCCTCGCCACATCTACCC
  GGCCGTGGAGTTCCTGGGGCCCTGCGAGCAGGGCGAGAGGAGAAACTCGGCTCCAGAATCCATCCTGCTG
  GTTCCGCCCACTTGGCCCAAGCCGCTGGTGCCTGCCATTCCCATCTGCAGCATCCCAGTGACTGCATCCC
  TCCCTCCACTTGAGTGGCCGCTGTCCAGTCAGTCAGGCTCTTACGAGCTGCGGATCGAGGTGCAGCCCAA
  GCCACATCACCGGGCCCACTATGAGACAGAAGGCAGCCGAGGGGCTGTCAAAGCTCCAACTGGAGGCCAC
  CCTGTGGTTCAGCTCCATGGCTACATGGAAAACAAGCCTCTGGGACTTCAGATCTTCATTGGGACAGCTG
  ATGAGCGGATCCTTAAGCCGCACGCCTTCTACCAGGTGCACCGAATCACGGGGAAAACTGTCACCACCAC
  CAGCTATGAGAAGATAGTGGGCAACACCAAAGTCCTGGAGATACCCTTGGAGCCCAAAAACAACATGAGG
  GCAACCATCGACTGTGCGGGGATCTTGAAGCTTAGAAACGCCGACATTGAGCTGCGGAAAGGCGAGACGG
  ACATTGGAAGAAAGAACACGCGGGTGAGACTGGTTTTCCGAGTTCACATCCCAGAGTCCAGTGGCAGAAT
  CGTCTCTTTACAGACTGCATCTAACCCCATCGAGTGCTCCCAGCGATCTGCTCACGAGCTGCCCATGGTT
  GAAAGACAAGACACAGACAGCTGCCTGGTCTATGGCGGCCAGCAAATGATCCTCACGGGGCAGAACTTTA
  CATCCGAGTCCAAAGTTGTGTTTACTGAGAAGACCACAGATGGACAGCAAATTTGGGAGATGGAAGCCAC
  GGTGGATAAGGACAAGAGCCAGCCCAACATGCTTTTTGTTGAGATCCCTGAATATCGGAACAAGCATATC
  CGCACACCTGTAAAAGTGAACTTCTACGTCATCAATGGGAAGAGAAAACGAAGTCAGCCTCAGCACTTTA
  CCTACCACCCAGTCCCAGCCATCAAGACGGAGCCCACGGATGAATATGACCCCACTCTGATCTGCAGCCC
  CACCCATGGAGGCCTGGGGAGCCAGCCTTACTACCCCCAGCACCCGATGGTGGCCGAGTCCCCCTCCTGC
  CTCGTGGCCACCATGGCTCCCTGCCAGCAGTTCCGCACGGGGCTCTCATCCCCTGACGCCCGCTACCAGC
  AACAGAACCCAGCGGCCGTACTCTACCAGCGGAGCAAGAGCCTGAGCCCCAGCCTGCTGGGCTATCAGCA
  GCCGGCCCTCATGGCCGCCCCGCTGTCCCTTGCGGACGCTCACCGCTCTGTGCTGGTGCACGCCGGCTCC
  CAGGGCCAGAGCTCAGCCCTGCTCCACCCCTCTCCGACCAACCAGCAGGCCTCGCCTGTGATCCACTACT
  CACCCACCAACCAGCAGCTGCGCTGCGGAAGCCACCAGGAGTTCCAGCACATCATGTACTGCGAGAATTT
  CGCACCAGGCACCACCAGACCTGGCCCGCCCCCGGTCAGTCAAGGTCAGAGGCTGAGCCCGGGTTCCTAC
  CCCACAGTCATTCAGCAGCAGAATGCCACGAGCCAAAGAGCCGCCAAAAACGGACCCCCGGTCAGTGACC
  AAAAGGAAGTATTACCTGCGGGGGTGACCATTAAACAGGAGCAGAACTTGGACCAGACCTACTTGGATGA
  TGAGCTGATAGACACACACCTTAGCTGGATACAAAACATATTATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001136021
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136021.2, NP_001129493.1
• RefSeq Size: 7452 bp
• RefSeq ORF: 2706 bp
• Locus ID: 4773
• Cytogenetics: 20q13.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: 'This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]'
  Transcript Variant: This variant (3) uses an alternate first exon and contains an alternate exon in the 3' end compared to variant 2, that causes a frameshift. The resulting isoform (D) has shorter and distinct N- and C-termini compared to isoform C. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.