Myosin Phosphatase (PPP1R12A) (NM_001143885) Human Untagged Clone

CAT#: SC326318

PPP1R12A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1R12A
• Synonyms: M130; MBS; MYPT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001143885, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAACGCTGGATCGGCTCCGAGACGGACC
  TCGAGCCTCCGGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCTGC
  TTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAAT
  GTGGACGGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTGGTAG
  AAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTACATGCAGCAGCTTCCTGTGG
  ATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCACATGTAGGGGCTGTCAACAGTGAAGGAGAT
  ACACCTTTAGATATTGCGGAGGAGGAGGCAATGGAAGAGCTACTTCAAAATGAAGTTAATCGGCAAGGGG
  TTGATATAGAAGCAGCTCGAAAGGAAGAAGAACGGATCATGCTTAGAGATGCCAGGCAGTGGCTAAATAG
  TGGTCATATAAATGATGTCCGGCATGCAAAATCTGGAGGTACAGCACTTCACGTTGCAGCTGCTAAAGGC
  TATACGGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGACTATGATGGCTGGA
  CACCTCTTCATGCTGCAGCTCATTGGGGTAAAGAAGAAGCATGTCGAATTTTAGTGGACAATCTGTGTGA
  TATGGAGATGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATTTTAGGATATTTA
  GAAGAGTTGCAAAAGAAACAAAATCTGCTCCATAGTGAAAAACGGGACAAGAAATCTCCACTAATTGAAT
  CAACAGCAAATATGGACAATAATCAGTCACAGAAGACCTTTAAAAACAAAGAGACGTTGATTATTGAACC
  AGAGAAAAATGCATCCCGTATTGAATCTCTGGAACAAGAAAAGGTTGATGAAGAAGAAGAAGGAAAGAAG
  GATGAGTCTAGCTGCTCTAGTGAAGAAGATGAGGAAGATGACTCGGAATCAGAAGCTGAAACAGATAAGA
  CAAAACCCCTGGCTTCTGTAACTAATGCCAACACTTCTAGTACACAAGCAGCTCCTGTAGCTGTTACAAC
  ACCTACTGTGTCATCAGGTCAAGCAACACCTACATCACCTATTAAAAAGTTTCCAACCACAGCTACAAAA
  ATTTCTCCCAAAGAAGAAGAGAGAAAAGATGAGTCTCCTGCAACTTGGAGGTTAGGACTTAGAAAGACGG
  GCAGCTATGGTGCACTTGCTGAAATCACAGCATCTAAAGAGGGTCAGAAAGAAAAAGATACTGCAGGTGT
  TACACGTTCAGCTTCAAGTCCCAGACTTTCCTCCTCTTTGGATAATAAAGAAAAGGAGAAAGATAGTAAA
  GGAACTAGGCTTGCATATGTTGCACCTACAATACCAAGACGACTAGCCAGTACATCTGACATTGAAGAGA
  AAGAAAACAGAGATTCTTCAAGTTTGCGAACAAGTAGTTCATATACAAGGAGAAAATGGGAAGATGATCT
  TAAAAAAAATAGCTCAGTTAATGAAGGATCAACGTATCATAAAAGTTGCTCCTTTGGTAGAAGACAAGAT
  GATTTGATTAGTTCTAGTGTTCCAAGCACCACATCAACACCAACAGTTACCTCTGCAGCTGGGCTTCAGA
  AAAGCCTGCTTTCCAGCACAAGCACTACTACAAAGATTACAACGGGTTCTTCCTCAGCAGGCACACAAAG
  CAGTACCTCAAATCGTTTGTGGGCTGAGGATAGTACTGAGAAAGAAAAGGACAGTGTTCCTACGGCAGTG
  ACCATTCCTGTTGCTCCAACTGTTGTAAATGCTGCAGCTTCTACCACAACCCTGACTACAACTACTGCTG
  GCACTGTCTCCTCCACAACAGAGGTCAGGGAGAGACGCAGATCATACCTCACTCCTGTTAGGGATGAAGA
  GTCTGAATCCCAAAGAAAAGCAAGATCTAGACAAGCAAGACAATCTAGAAGATCAACACAGGGAGTGACA
  TTAACTGATCTTCAAGAAGCTGAGAAAACAATAGGAAGAAGTCGTTCTACCCGAACCAGAGAACAAGAAA
  ATGAAGAAAAAGAAAAAGAGGAAAAAGAGAAACAAGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGA
  AACATCTAGAGAAGATGAATATAAACAAAAGTACTCCAGAACGTATGATGAGACTTACCAGCGTTATAGG
  CCAGTATCAACTTCAAGTTCAACCACTCCATCCTCTTCACTTTCTACTATGAGCAGTTCACTGTATGCTT
  CAAGTCAACTAAACAGGCCAAATAGTCTTGTAGGCATAACTTCTGCTTACTCCAGAGGAATAACAAAAGA
  AAATGAAAGAGAGGGAGAAAAAAGAGAAGAGGAGAAAGAAGGAGAAGATAAATCACAACCTAAATCAATC
  AGAGAACGACGACGACCAAGAGAGAAAAGAAGATCTACAGGAGTTTCATTTTGGACACAAGATAGTGATG
  AAAATGAACAAGAACAACAATCAGACACAGAAGAGGGATCCAATAAGAAAGAAACTCAGACGGATTCCAT
  TTCTAGATATGAAACCAGTTCTACATCAGCTGGTGATCGATATGATTCCTTGCTGGGTCGCTCTGGATCA
  TACAGTTACTTAGAAGAAAGAAAACCTTACAGCAGCAGGCTAGAAAAGGATGACTCAACTGACTTTAAAA
  AGCTTTATGAACAAATTCTAGCTGAAAATGAAAAGCTGAAGGCACAGCTACATGATACAAATATGGAACT
  AACAGATCTTAAATTACAGTTGGAAAAGGCCACCCAGAGACAAGAAAGATTTGCTGATAGATCACTGTTG
  GAAATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGAAGAATATCTGAAATGGAAGAAGAGCTCAAAATGT
  TACCAGACCTAAAAGCAGACAACCAGAGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAA
  ACTTTCCAAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001143885
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001143885.1, NP_001137357.1
• RefSeq Size: 5582 bp
• RefSeq ORF: 3093 bp
• Locus ID: 4659
• Cytogenetics: 12q21.2-q21.31
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: 'Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.