DEPDC5 (NM_001136029) Human Untagged Clone

CAT#: SC326392

DEPDC5 (untagged)-Human DEP domain containing 5 (DEPDC5), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DEPDC5
• Synonyms: DEP.5; FFEVF; FFEVF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001136029, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGAACAACAAAGGTCTACAAACTCGTCATCCACAAGAAGGGCTTTGGGGGCAGTGATGATGAGCTAG
  TTGTGAACCCCAAAGTGTTCCCTCACATCAAGCTTGGAGACATTGTAGAGATTGCACACCCCAACGATGA
  ATACAGCCCTCTGCTTTTGCAGGTCAAGTCTCTTAAGGAAGATTTACAGAAGGAAACTATCAGTGTGGAC
  CAGACTGTGACTCAAGTGTTCCGGCTGAGACCTTATCAGGATGTCTATGTTAATGTCGTAGACCCTAAGG
  ATGTGACCCTTGACCTAGTGGAATTAACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGGCGACT
  AAAGAAAAGTTTGGTCAGCACATGTGCCTATATCACCCAGAAGGTGGAGTTTGCTGGCATCAGAGCACAG
  GCTGGTGAACTGTGGGTTAAGAATGAGAAGGTCATGTGTGGCTACATCAGTGAAGATACCAGGGTGGTGT
  TTCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGATTTTGATATTTA
  TGGGGATTTGTATTTTGAGAAAGCTGTGAATGGTTTCCTTGCTGATCTATTTACCAAGTGGAAGGAGAAG
  AACTGTAGTCATGAAGTGACAGTGGTCCTGTTTTCTAGAACTTTCTATGATGCAAAATCTGTTGATGAAT
  TTCCTGAAATAAACCGAGCCTCAATTCGACAGGATCACAAGGGGAGATTCTATGAAGACTTTTACAAAGT
  GGTGGTGCAGAATGAGAGAAGAGAAGAATGGACTTCACTTCTCGTAACCATTAAAAAACTCTTCATCCAG
  TATCCAGTGTTGGTGCGACTGGAACAGGCAGAGGGCTTTCCTCAAGGAGATAATTCTACCTCAGCACAAG
  GAAACTACCTGGAGGCCATCAATCTGTCATTCAATGTGTTTGATAAGCACTACATCAACCGCAACTTTGA
  CCGAACTGGGCAGATGTCAGTGGTGATCACGCCCGGGGTGGGTGTCTTTGAAGTGGACCGCCTACTCATG
  ATCCTGACCAAGCAGCGGATGATAGATAATGGAATTGGTGTGGATTTGGTGTGCATGGGAGAGCAACCGT
  TACATGCTGTCCCATTGTTCAAGCTCCATAATCGGAGTGCTCCCCGTGATTCTCGTCTGGGCGATGACTA
  TAATATCCCTCACTGGATAAACCACAGTTTCTACACATCCAAAAGCCAGCTCTTTTGTAATAGTTTCACC
  CCACGAATAAAACTGGCAGGAAAGAAGCCCGCCTCTGAGAAAGCAAAAAATGGCCGTGATACATCTCTCG
  GGAGTCCAAAAGAATCTGAGAACGCCCTTCCCATCCAAGTAGATTATGACGCCTATGACGCTCAAGTGTT
  CAGGCTGCCCGGCCCATCCCGGGCCCAGTGCCTCACCACCTGCAGATCTGTGCGAGAGCGAGAGAGTCAC
  AGTCGAAAGAGTGCCAGCTCCTGTGATGTTTCATCCAGCCCTTCCCTACCAAGCCGCACACTGCCCACTG
  AGGAAGTGAGGAGCCAGGCTTCTGACGACAGCTCCCTAGGCAAGAGTGCCAACATCCTGATGATCCCACA
  CCCCCACCTGCACCAGTATGAAGTCAGCAGCTCCTTGGGATACACCAGCACTCGAGATGTCCTGGAGAAC
  ATGATGGAGCCACCACAGCGAGACTCCAGTGCACCAGGGAGGTTTCACGTTGGCAGTGCAGAATCCATGC
  TGCATGTTCGACCTGGTGGATACACGCCCCAGAGAGCACTGATTAACCCCTTCGCTCCCTCTCGGATGCC
  CATGAAGCTTACGTCCAACAGAAGGCGCTGGATGCACACTTTTCCTGTGGGGCCATCCGGAGAAGCCATC
  CAGATCCACCACCAGACCCGACAGAATATGGCGGAGCTACAAGGCAGCGGGCAGAGGGATCCAACTCACT
  CCTCTGCAGAGCTGCTGGAGTTAGCATATCATGAAGCTGCTGGAAGGCACAGCAATTCCCGCCAGCCTGG
  TGACGGCATGTCCTTCTTGAACTTCAGTGGAACAGAGGAGCTTTCTGTCGGCCTGCTTAGCAACAGTGGT
  GCAGGTATGAATCCTAGGACCCAGAATAAGGATTCTCTAGAGGACAGTGTTTCTACCTCTCCAGACCCAA
  TGCCAGGCTTCTGTTGCACAGTTGGAGTGGACTGGAAGTCTCTCACTACTCCGGCGTGCCTCCCCCTTAC
  CACCGACTACTTCCCTGACCGCCAGGGCCTGCAGAATGACTACACAGAGGGCTGTTATGATCTCCTTCCA
  GAAGCAGACATCGACAGGAGGGACGAAGATGGTGTGCAGATGACAGCCCAGCAGGTATTTGAAGAGTTTA
  TTTGCCAACGTCTCATGCAGGGCTACCAAATCATAGTGCAGCCCAAGACACAGAAACCCAATCCTGCTGT
  CCCGCCCCCGCTGAGCAGTAGCCCACTCTATAGCCGAGGCCTTGTGTCCCGAAACCGCCCTGAGGAGGAG
  GACCAGTATTGGCTGAGTATGGGCAGAACGTTCCACAAAGTGACGCTGAAGGATAAGATGATCACAGTGA
  CGCGATACCTTCCCAAGTATCCTTATGAATCTGCCCAGATCCACTACACCTACAGCCTCTGTCCTTCCCA
  CTCAGACTCAGAGTTCGTCTCCTGCTGGGTGGAATTCTCCCACGAACGGCTGGAGGAGTACAAGTGGAAT
  TACTTAGATCAGTATATCTGTTCTGCCGGCTCTGAAGACTTCAGCTTAATTGAGTCCCTGAAGTTCTGGA
  GGACCCGCTTCCTGCTGCTGCCAGCCTGTGTCACCGCCACCAAGCGCATCACGGAGGGGGAGGCCCACTG
  CGACATCTATGGGGACAGGCCCCGTGCAGACGAGGACGAGTGGCAACTCCTGGATGGTTTTGTCCGCTTT
  GTGGAGGGCTTGAATCGCATTCGCAGGCGGCATCGCTCGGATCGCATGATGCGGAAAGGGACCGCCATGA
  AAGGCTTGCAGATGACTGGGCCCATTTCCACGCATTCTCTGGAGTCAACTGCACCCCCAGTGGGGAAGAA
  GGGAACCTCAGCTCTCTCTGCCCTGTTGGAGATGGAGGCCAGTCAGAAGTGCCTGGGAGAACAGCAGGCA
  GCTGTGCATGGTGGGAAGAGCTCCGCCCAGTCAGCCGAGAGCAGCAGCGTTGCCATGACTCCCACCTACA
  TGGACAGCCCACGAAAGGACGGGGCCTTCTTTATGGAGTTTGTCCGCAGCCCACGCACAGCATCGTCCGC
  CTTCTACCCTCAGGTATCTGTGGACCAAACAGCCACTCCTATGTTGGACGGCACCAGTTTGGGCATATGC
  ACAGGCCAATCCATGGACAGAGGCAACAGCCAGACCTTTGGGAACTCCCAGAACATAGGAGAACAGGGCT
  ACTCCTCCACAAACTCCAGTGACAGCAGCTCTCAGCAGCTGGTGGCAAGCTCCTTGACCTCATCCTCTAC
  CCTGACAGAGATCCTGGAAGCCATGAAGCACCCCTCGACAGGAGTCCAGCTGCTCTCTGAACAGAAGGGC
  CTCTCACCGTACTGCTTCATCAGCGCGGAGGTGGTACACTGGTTGGTGAACCACGTGGAGGGGATCCAGA
  CACAGGCGATGGCCATTGACATCATGCAGAAAATGCTGGAAGAGCAGCTCATCACACATGCATCTGGCGA
  AGCCTGGCGGACCTTCATCTACGGCTTCTATTTCTACAAGATAGTAACGGACAAAGAGCCCGACCGAGTG
  GCCATGCAGCAGCCCGCCACCACCTGGCACACAGCAGGAGTGGACGACTTCGCCAGCTTCCAGCGCAAGT
  GGTTTGAGGTGGCCTTTGTGGCAGAAGAGCTCGTGCACTCTGAGATTCCTGCCTTTCTCCTGCCCTGGCT
  GCCTAGCCGGCCAGCCTCCTATGCAAGTAGGCACAGCTCCTTTAGCCGAAGTTTTGGAGGACGGAGCCAG
  GCGGCAGCACTTTTAGCTGCCACTGTCCCAGAGCAGAGGACTGTGACCCTGGATGTTGACGTGAACAACC
  GCACAGACCGGCTGGAGTGGTGCAGCTGTTATTACCATGGCAACTTTTCTCTGAATGCAGCCTTTGAGAT
  CAAGCTGCACTGGATGGCGGTGACCGCAGCAGTACTCTTCGAGATGGTCCAAGGTTGGCATCGGAAAGCC
  ACCTCCTGTGGCTTCTTGTTAGTCCCAGTTTTGGAGGGGCCTTTTGCACTGCCCAGTTACCTGTATGGCG
  ACCCCCTTCGTGCCCAGCTCTTCATCCCACTCAACATCAGCTGCTTGCTCAAGGAGGGCAGCGAGCACCT
  GTTTGATAGCTTTGAACCCGAAACGTACTGGGATCGAATGCACCTCTTCCAGGAAGCCATTGCACACAGG
  TTTGGGTTTGTACAAGATAAATATTCTGCCTCTGCTTTTAACTTCCCTGCTGAGAACAAGCCTCAGTATA
  TCCACGTTACAGGAACAGTGTTTCTGCAGCTGCCCTACTCCAAGCGCAAGTTCTCAGGGCAGCAGCGGCG
  GCGGCGGAACTCCACCAGCTCCACCAACCAGAACATGTTCTGCGAGGAGCGGGTCGGCTACAACTGGGCC
  TACAACACCATGCTCACCAAAACATGGCGCTCCAGCGCCACAGGGGATGAAAAGTTTGCTGATCGGCTGC
  TGAAGGACTTCACGGACTTCTGCATCAACCGTGACAACCGGCTGGTCACGTTCTGGACAAGTTGCCTGGA
  GAAGATGCATGCCAGTGCCCCGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001136029
• ORF Size: 4785 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001136029.2, NP_001129501.1
• RefSeq Size: 5382
• RefSeq ORF: 4785
• Locus ID: 9681
• Gene Summary: This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in an internal exon compared to variant 4. The encoded protein (isoform 3) is shorter than isoform 4.