MYO5A (NM_001142495) Human Untagged Clone

CAT#: SC326405

MYO5A (untagged)-Human myosin VA (heavy chain 12, myoxin) (MYO5A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYO5A
• Synonyms: GS1; MYH12; MYO5; MYR12
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001142495, the custom clone sequence may differ by one or more nucleotides
  ATGGCTGCGTCGGAGCTCTACACAAAGTTTGCCAGGGTTTGGATACCTGATCCAGAGGAA
  GTCTGGAAGTCAGCAGAGCTGCTCAAAGATTATAAGCCAGGAGATAAAGTCCTCCTGCTT
  CACCTCGAGGAAGGAAAGGATTTGGAATACCATCTAGATCCAAAGACCAAGGAGCTGCCT
  CACTTACGAAATCCTGACATACTTGTTGGTGAAAATGACCTCACAGCCCTCAGCTATCTT
  CATGAGCCTGCTGTGCTCCATAATCTCAGAGTCCGCTTTATTGATTCCAAACTTATTTAT
  ACGTATTGTGGTATAGTCCTAGTAGCTATAAATCCCTATGAACAGCTGCCTATTTATGGA
  GAAGATATTATTAATGCATACAGTGGTCAGAACATGGGTGATATGGATCCACATATCTTT
  GCAGTAGCTGAAGAAGCTTACAAGCAAATGGCCAGAGATGAACGAAATCAGTCCATCATC
  GTAAGTGGAGAGTCTGGGGCAGGAAAAACAGTCTCAGCTAAGTATGCCATGCGATACTTT
  GCAACTGTGAGTGGTTCTGCCAGTGAGGCCAATGTGGAGGAAAAGGTCTTGGCCTCCAAC
  CCCATCATGGAGTCCATTGGAAATGCTAAAACAACCAGGAATGATAATAGCAGCCGTTTT
  GGGAAGTATATTGAGATTGGTTTTGATAAGAGATATCGAATCATTGGTGCCAATATGAGA
  ACTTATCTTTTAGAGAAATCCAGAGTGGTATTCCAGGCAGAAGAGGAGAGAAACTATCAT
  ATCTTCTATCAGCTTTGTGCCTCAGCAAAGTTACCTGAATTTAAAATGCTACGATTAGGA
  AATGCAGATAACTTTAATTACACAAAACAAGGAGGCAGTCCTGTGATTGAAGGAGTGGAT
  GATGCAAAGGAGATGGCACATACTAGGCAGGCCTGCACTTTGCTAGGAATTAGTGAATCT
  CATCAAATGGGAATTTTCCGAATACTTGCTGGCATCCTTCACTTAGGCAATGTTGGATTT
  ACATCCCGAGATGCAGACAGCTGCACAATACCTCCCAAGCATGAACCTCTCTGCATCTTC
  TGTGACCTCATGGGTGTGGACTATGAGGAGATGTGTCACTGGCTCTGCCATCGGAAACTG
  GCTACTGCCACAGAGACATACATCAAGCCCATCTCCAAGCTGCAGGCCACGAATGCCCGC
  GATGCTTTGGCCAAGCACATCTATGCCAAGCTCTTTAACTGGATTGTAGATAATGTCAAT
  CAGGCTCTCCATTCTGCTGTCAAACAGCACTCTTTTATTGGTGTGCTAGACATTTACGGA
  TTTGAAACATTTGAGATAAATAGTTTTGAACAGTTTTGCATAAATTATGCAAATGAAAAA
  CTACAGCAACAATTCAATATGCATGTCTTCAAATTGGAGCAAGAAGAATATATGAAGGAA
  CAAATTCCATGGACACTCATAGATTTTTATGATAATCAGCCTTGTATTAATCTTATAGAA
  TCAAAACTAGGCATTCTAGATTTACTGGATGAGGAATGCAAGATGCCTAAAGGCACAGAT
  GACACCTGGGCCCAAAAATTGTACAACACACATTTGAACAAATGTGCACTCTTTGAAAAG
  CCTCGTCTATCAAACAAAGCTTTCATCATCCAACATTTTGCTGACAAAGTGGAATACCAG
  TGTGAAGGATTTCTCGAAAAGAATAAAGACACCGTTTTTGAAGAACAAATTAAAGTTCTT
  AAATCAAGCAAGTTTAAGATGCTACCAGAACTATTTCAAGATGATGAGAAGGCCATCAGT
  CCAACTTCAGCCACCTCCTCAGGGCGCACACCCCTCACACGAACTCCTGCAAAGCCCACC
  AAAGGCAGACCAGGCCAAATGGCCAAAGAGCACAAGAAAACAGTGGGGCATCAGTTCAGA
  AACTCCCTGCACCTGCTTATGGAGACACTCAATGCCACTACCCCTCACTATGTGCGCTGT
  ATCAAGCCTAATGACTTCAAGTTCCCATTCACGTTTGATGAGAAGAGGGCAGTGCAGCAG
  CTGAGAGCATGTGGTGTCCTGGAAACCATCCGAATCAGTGCGGCCGGTTTCCCCTCACGG
  TGGACTTACCAAGAATTTTTCAGCCGCTACCGTGTCCTAATGAAGCAGAAAGATGTGCTG
  AGTGACAGAAAGCAAACATGCAAGAATGTGTTAGAGAAACTGATACTGGACAAGGACAAA
  TACCAGTTTGGTAAGACAAAGATCTTTTTCCGTGCCGGTCAAGTGGCCTATCTAGAAAAA
  TTGAGAGCTGACAAACTGAGAGCTGCCTGCATCCGGATCCAGAAGACCATCCGAGGGTGG
  CTGCTGAGAAAGAAGTACCTACGCATGCGGAAGGCAGCCATCACCATGCAGAGATACGTG
  CGGGGCTACCAGGCCCGATGCTATGCTAAGTTTCTGCGCAGAACCAAGGCAGCAACCATC
  ATTCAAAAGTACTGGCGCATGTATGTGGTCCGCAGGAGGTACAAGATTAGACGAGCTGCC
  ACTATTGTTCTTCAGTCTTACTTGCGAGGCTTCTTGGCCAGAAATAGGTATCGCAAGATA
  CTCCGTGAGCACAAAGCAGTCATCATTCAGAAGCGAGTCCGGGGCTGGCTGGCCCGCACA
  CACTACAAGAGGAGCATGCATGCCATCATCTACCTTCAGTGCTGCTTCAGGCGGATGATG
  GCCAAGCGTGAGCTAAAGAAGCTCAAAATCGAGGCTCGCTCAGTGGAGCGCTATAAGAAG
  CTGCACATCGGCATGGAGAACAAGATCATGCAGCTGCAGCGCAAAGTTGATGAGCAGAAC
  AAAGACTACAAATGCCTTGTGGAGAAACTAACCAATCTGGAAGGAATATACAACTCTGAG
  ACTGAGAAACTACGAAGTGACTTAGAACGTCTTCAACTAAGTGAAGAGGAAGCGAAAGTT
  GCCACTGGGCGGGTCCTTAGTCTGCAGGAAGAAATTGCCAAGCTCCGGAAAGACCTGGAG
  CAAACTCGTTCAGAGAAAAAATGCATTGAGGAACATGCAGATCGATACAAACAAGAAACA
  GAGCAGCTGGTATCAAATCTGAAGGAAGAAAATACTTTGCTGAAGCAAGAAAAAGAAGCC
  CTCAATCACCGCATCGTGCAGCAGGCTAAGGAGATGACAGAAACTATGGAGAAGAAGTTA
  GTAGAAGAAACGAAACAACTGGAACTCGACCTTAATGATGAAAGGCTGAGATATCAGAAC
  CTTCTGAATGAGTTCAGTCGCCTGGAAGAAAGATATGATGACCTCAAGGAAGAGATGACC
  CTTATGGTGCATGTGCCTAAGCCTGGACACAAGAGAACAGACTCCACCCACAGCAGCAAC
  GAGTCTGAATATATCTTTAGCTCTGAAATTGCAGAAATGGAAGACATTCCATCAAGGACA
  GAGGAACCAAGTGAGAAGAAGGTACCTCTGGACATGTCATTGTTCCTTAAGCTCCAGAAG
  CGGGTCACAGAGCTGGAGCAGGAGAAGCAGGTGATGCAGGATGAGCTGGACCGCAAGGAG
  GAGCAGGTGCTCCGCAGCAAGGCCAAGGAAGAAGAAAGACCACAAATTAGAGGTGCAGAA
  CTGGAATATGAGTCACTCAAGCGTCAAGAACTAGAATCAGAAAACAAAAAACTGAAGAAT
  GAGCTAAATGAGTTGCGCAAGGCCCTCAGTGAGAAAAGTGCCCCAGAGGTGACCGCCCCA
  GGTGCACCTGCCTACCGTGTCCTCATGGAGCAGCTGACCTCTGTGAGCGAGGAGCTTGAT
  GTCCGCAAGGAGGAAGTCCTCATCTTAAGGTCTCAACTGGTGAGCCAGAAAGAGGCCATC
  CAACCCAAGGATGACAAGAATACAATGACAGATTCCACAATACTTTTGGAAGATGTACAA
  AAAATGAAAGATAAAGGTGAAATAGCACAAGCATACATTGGTTTGAAAGAAACAAATAGG
  CTCCTGGAATCCCAGCTGCAGTCACAGAAGAGGAGCCATGAGAATGAGGCCGAGGCCCTC
  CGTGGGGAGATCCAGAGCCTGAAGGAGGAGAACAACCGACAGCAGCAGCTGCTGGCCCAG
  AACCTGCAGCTGCCCCCAGAGGCCCGCATTGAGGCCAGCCTGCAGCACGAGATCACCCGG
  CTGACCAACGAAAACTTGGATTTGATGGAACAACTTGAAAAACAGGATAAGACGGTCCGT
  AAACTGAAAAAACAACTGAAAGTATTTGCCAAAAAAATTGGCGAACTAGAAGTGGGCCAG
  ATGGAGAACATATCCCCAGGACAGATCATTGATGAACCCATCCGACCAGTCAACATTCCC
  AGGAAAGAAAAGGATTTCCAAGGGATGCTGGAATACAAGAAGGAGGATGAGCAAAAACTT
  GTTAAGAACCTGATTCTGGAACTGAAGCCACGTGGTGTAGCAGTCAATTTGATTCCAGGA
  TTACCGGCATATATCCTGTTCATGTGTGTTCGACATGCTGACTACCTGAATGATGATCAG
  AAAGTAAGGTCGTTGCTAACATCAACAATTAACAGCATCAAAAAAGTATTGAAGAAAAGA
  GGTGATGATTTTGAAACCGTCTCCTTCTGGCTCTCTAACACATGCCGATTTTTGCACTGC
  TTGAAACAGTACAGTGGAGAAGAGGGCTTTATGAAGCACAACACATCTCGCCAGAATGAA
  CACTGCCTCACCAATTTTGACCTGGCTGAGTATCGGCAGGTGCTGAGTGACTTGGCCATT
  CAGATCTACCAGCAGCTCGTGCGGGTGTTAGAGAACATCCTTCAGCCAATGATTGTCTCA
  GGCATGCTGGAACATGAAACGATTCAGGGCGTGTCTGGGGTGAAGCCCACAGGGTTGAGA
  AAGCGAACCTCCAGTATCGCCGATGAGGGCACCTACACACTGGACTCCATCCTCCGGCAG
  CTCAACTCCTTCCACTCGGTCATGTGTCAGCATGGCATGGACCCTGAACTGATCAAGCAG
  GTGGTCAAGCAGATGTTCTACATCATAGGGGCCATCACCCTGAACAACCTTCTCCTGCGG
  AAGGACATGTGCTCCTGGAGTAAAGGCATGCAGATCAGGTACAATGTCAGTCAACTGGAA
  GAATGGCTGCGTGACAAGAATCTGATGAATAGTGGGGCTAAAGAAACCCTGGAACCTCTC
  ATTCAGGCTGCTCAACTTTTGCAAGTGAAAAAGAAAACAGATGATGATGCAGAAGCCATT
  TGTTCTATGTGCAATGCTTTAACTACTGCCCAGATTGTGAAAGTGTTGAATTTGTATACT
  CCAGTTAATGAGTTTGAAGAAAGAGTCTCTGTGTCGTTCATTCGTACTATACAGATGCGT
  TTACGAGACAGGAAAGACTCTCCCCAGCTGCTCATGGATGCTAAACACATCTTTCCTGTC
  ACCTTTCCTTTCAACCCATCTTCCCTCGCACTAGAAACCATCCAGATTCCAGCCAGCCTC
  GGCCTGGGCTTCATTTCACGGGTC
  
• Restriction Sites: Please inquire
• ACCN: NM_001142495
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142495.1, NP_001135967.1
• RefSeq Size: 12157 bp
• RefSeq ORF: 5487 bp
• Locus ID: 4644
• Cytogenetics: 15q21.2
• Gene Summary: 'This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the CDS, resulting in a shorter isoform (2), as compared to variant 1.