CD133 (PROM1) (NM_001145847) Human Untagged Clone

CAT#: SC326502

PROM1 (untagged)-Human prominin 1 (PROM1), transcript variant 2, mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PROM1
• Synonyms: AC133; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145847, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCTCGTACTCGGCTCCCTGTTGCTGCTGGGGCTGTGCGGGAACTCCTTTTCAGGAGGGCAGCCTT
  CATCCACAGATGCTCCTAAGGCTTGGAATTATGAATTGCCTGCAACAAATTATGAGACCCAAGACTCCCA
  TAAAGCTGGACCCATTGGCATTCTCTTTGAACTAGTGCATATCTTTCTCTATGTGGTACAGCCGCGTGAT
  TTCCCAGAAGATACTTTGAGAAAATTCTTACAGAAGGCATATGAATCCAAAATTGATTATGACAAGATTG
  TCTACTATGAAGCAGGGATTATTCTATGCTGTGTCCTGGGGCTGCTGTTTATTATTCTGATGCCTCTGGT
  GGGGTATTTCTTTTGTATGTGTCGTTGCTGTAACAAATGTGGTGGAGAAATGCACCAGCGACAGAAGGAA
  AATGGGCCCTTCCTGAGGAAATGCTTTGCAATCTCCCTGTTGGTGATTTGTATAATAATAAGCATTGGCA
  TCTTCTATGGTTTTGTGGCAAATCACCAGGTAAGAACCCGGATCAAAAGGAGTCGGAAACTGGCAGATAG
  CAATTTCAAGGACTTGCGAACTCTCTTGAATGAAACTCCAGAGCAAATCAAATATATATTGGCCCAGTAC
  AACACTACCAAGGACAAGGCGTTCACAGATCTGAACAGTATCAATTCAGTGCTAGGAGGCGGAATTCTTG
  ACCGACTGAGACCCAACATCATCCCTGTTCTTGATGAGATTAAGTCCATGGCAACAGCGATCAAGGAGAC
  CAAAGAGGCGTTGGAGAACATGAACAGCACCTTGAAGAGCTTGCACCAACAAAGTACACAGCTTAGCAGC
  AGTCTGACCAGCGTGAAAACTAGCCTGCGGTCATCTCTCAATGACCCTCTGTGCTTGGTGCATCCATCAA
  GTGAAACCTGCAACAGCATCAGATTGTCTCTAAGCCAGCTGAATAGCAACCCTGAACTGAGGCAGCTTCC
  ACCCGTGGATGCAGAACTTGACAACGTTAATAACGTTCTTAGGACAGATTTGGATGGCCTGGTCCAACAG
  GGCTATCAATCCCTTAATGATATACCTGACAGAGTACAACGCCAAACCACGACTGTCGTAGCAGGTATCA
  AAAGGGTCTTGAATTCCATTGGTTCAGATATCGACAATGTAACTCAGCGTCTTCCTATTCAGGATATACT
  CTCAGCATTCTCTGTTTATGTTAATAACACTGAAAGTTACATCCACAGAAATTTACCTACATTGGAAGAG
  TATGATTCATACTGGTGGCTGGGTGGCCTGGTCATCTGCTCTCTGCTGACCCTCATCGTGATTTTTTACT
  ACCTGGGCTTACTGTGTGGCGTGTGCGGCTATGACAGGCATGCCACCCCGACCACCCGAGGCTGTGTCTC
  CAACACCGGAGGCGTCTTCCTCATGGTTGGAGTTGGATTAAGTTTCCTCTTTTGCTGGATATTGATGATC
  ATTGTGGTTCTTACCTTTGTCTTTGGTGCAAATGTGGAAAAACTGATCTGTGAACCTTACACGAGCAAGG
  AATTATTCCGGGTTTTGGATACACCCTACTTACTAAATGAAGACTGGGAATACTATCTCTCTGGGAAGCT
  ATTTAATAAATCAAAAATGAAGCTCACTTTTGAACAAGTTTACAGTGACTGCAAAAAAAATAGAGGCACT
  TACGGCACTCTTCACCTGCAGAACAGCTTCAATATCAGTGAACATCTCAACATTAATGAGCATACTGGAA
  GCATAAGCAGTGAATTGGAAAGTCTGAAGGTAAATCTTAATATCTTTCTGTTGGGTGCAGCAGGAAGAAA
  AAACCTTCAGGATTTTGCTGCTTGTGGAATAGACAGAATGAATTATGACAGCTACTTGGCTCAGACTGGT
  AAATCCCCCGCAGGAGTGAATCTTTTATCATTTGCATATGATCTAGAAGCAAAAGCAAACAGTTTGCCCC
  CAGGAAATTTGAGGAACTCCCTGAAAAGAGATGCACAAACTATTAAAACAATTCACCAGCAACGAGTCCT
  TCCTATAGAACAATCACTGAGCACTCTATACCAAAGCGTCAAGATACTTCAACGCACAGGGAATGGATTG
  TTGGAGAGAGTAACTAGGATTCTAGCTTCTCTGGATTTTGCTCAGAACTTCATCACAAACAATACTTCCT
  CTGTTATTATTGAGGAAACTAAGAAGTATGGGAGAACAATAATAGGATATTTTGAACATTATCTGCAGTG
  GATCGAGTTCTCTATCAGTGAGAAAGTGGCATCGTGCAAACCTGTGGCCACCGCTCTAGATACTGCTGTT
  GATGTCTTTCTGTGTAGCTACATTATCGACCCCTTGAATTTGTTTTGGTTTGGCATAGGAAAAGCTACTG
  TATTTTTACTTCCGGCTCTAATTTTTGCGGTAAAACTGGCTAAGTACTATCGTCGAATGGATTCGGAGGA
  CGTGTACGATGATGTTGAAACTATACCCATGAAAAATATGGAAAATGGTAATAATGGTTATCATAAAGAT
  CATGTATATGGTATTCACAATCCTGTTATGACAAGCCCATCACAACATTGA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_001145847
• ORF Size: 2571 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001145847.1, NP_001139319.1
• RefSeq Size: 4257
• RefSeq ORF: 2571
• Locus ID: 8842
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (2) represents one of several alternative promoters and 5' UTRs, and lacks an alternative in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (2), also known as s1, lacks a 9 aa segment near the N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.