WFS1 (NM_001145853) Human Untagged Clone

CAT#: SC326504

WFS1 (untagged)-Human Wolfram syndrome 1 (wolframin) (WFS1), transcript variant 2, mRNA


  "NM_001145853" in other vectors (6)

Reconstitution Protocol

USD 760.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol WFS1
Synonyms CTRCT41; WFRS; WFS; WFSL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001145853, the custom clone sequence may differ by one or more nucleotides


ATGGACTCCAACACTGCTCCGCTGGGCCCCTCCTGCCCACAGCCCCCGCCAGCACCGCAGCCCCAGGCGC
GTTCCCGACTCAATGCCACAGCCTCGTTGGAGCAGGAGAGGAGCGAAAGGCCCCGAGCACCCGGACCCCA
GGCTGGCCCTGGCCCTGGTGTTAGAGACGCAGCGGCCCCCGCTGAACCCCAGGCCCAGCATACCAGGAGC
CGGGAAAGAGCAGACGGCACCGGGCCTACAAAGGGAGACATGGAAATCCCCTTTGAAGAAGTCCTGGAGA
GGGCCAAGGCCGGGGACCCCAAGGCACAGACTGAGGTGGGGAAGCACTACCTGCAGTTGGCCGGCGACAC
GGATGAAGAACTCAACAGCTGCACCGCTGTGGACTGGCTGGTCCTCGCCGCGAAGCAGGGCCGTCGCGAG
GCTGTGAAGCTGCTTCGCCGGTGCTTGGCGGACAGAAGAGGCATCACGTCCGAGAACGAACGGGAGGTGA
GGCAGCTCTCCTCCGAGACCGACCTGGAGAGGGCCGTGCGCAAGGCAGCCCTGGTCATGTACTGGAAGCT
CAACCCCAAGAAGAAGAAGCAGGTGGCCGTGGCGGAGCTGCTGGAGAATGTCGGCCAGGTCAACGAGCAC
GATGGAGGGGCGCAGCCAGGCCCCGTGCCCAAGTCCCTGCAGAAGCAGAGGCGCATGCTGGAGCGCCTGG
TCAGCAGCGAGTCCAAGAACTACATCGCGCTGGATGACTTTGTGGAGATCACTAAGAAGTACGCCAAGGG
CGTCATCCCCAGCAGCCTGTTCCTGCAGGACGACGAAGATGATGACGAGCTGGCGGGGAAGAGCCCTGAG
GACCTGCCACTGCGTCTGAAGGTGGTCAAGTACCCCCTGCACGCCATCATGGAGATCAAGGAGTACCTGA
TTGACATGGCCTCCAGGGCAGGCATGCACTGGCTGTCCACCATCATCCCCACGCACCACATCAACGCGCT
CATCTTCTTCTTCATCGTCAGCAACCTCACCATCGACTTCTTCGCCTTCTTCATCCCGCTGGTCATCTTC
TACCTGTCCTTCATCTCCATGGTGATCTGCACCCTCAAGGTGTTCCAGGACAGCAAGGCCTGGGAGAACT
TCCGCACCCTCACCGACCTGCTGCTGCGCTTCGAGCCCAACCTGGATGTGGAGCAGGCCGAGGTCAACTT
CGGCTGGAACCACCTGGAGCCCTATGCCCATTTCCTGCTCTCTGTCTTCTTCGTCATCTTCTCCTTCCCC
ATCGCCAGCAAGGACTGCATCCCCTGCTCGGAGCTGGCTGTCATCACCGGCTTCTTTACCGTGACCAGCT
ACCTGAGCCTGAGCACCCATGCAGAGCCCTACACGCGCAGGGCCCTGGCCACCGAGGTCACCGCCGGCCT
GCTATCGCTGCTGCCCTCCATGCCCTTGAATTGGCCCTACCTGAAGGTCCTTGGCCAGACCTTCATCACC
GTGCCTGTCGGCCACCTGGTCGTCCTCAACGTCAGCGTCCCGTGCCTGCTCTATGTCTACCTGCTCTATC
TCTTCTTCCGCATGGCACAGCTGAGGAATTTCAAGGGCACCTACTGCTACCTTGTGCCCTACCTGGTGTG
CTTCATGTGGTGTGAGCTCTCCGTGGTCATCCTGCTGGAGTCCACCGGCCTGGGGCTGCTCCGCGCCTCC
ATCGGCTACTTCCTCTTCCTCTTTGCCCTCCCCATCCTGGTGGCCGGCCTGGCCCTGGTGGGCGTGCTGC
AGTTCGCCCGGTGGTTCACGTCTCTGGAGCTCACCAAGATCGCAGTCACCGTGGCGGTCTGTAGTGTGCC
CCTGCTGTTGCGCTGGTGGACCAAGGCCAGCTTCTCTGTGGTGGGGATGGTGAAGTCCCTGACGCGGAGC
TCCATGGTCAAGCTCATCCTGGTGTGGCTCACGGCCATCGTGCTGTTCTGCTGGTTCTATGTGTACCGCT
CAGAGGGCATGAAGGTCTACAACTCCACACTGACCTGGCAGCAGTATGGTGCGCTGTGCGGGCCACGCGC
CTGGAAGGAGACCAACATGGCGCGCACCCAGATCCTCTGCAGCCACCTGGAGGGCCACAGGGTCACGTGG
ACCGGCCGCTTCAAGTACGTCCGCGTGACTGACATCGACAACAGCGCCGAGTCTGCCATCAACATGCTCC
CGTTCTTCATCGGCGACTGGATGCGCTGCCTCTACGGCGAGGCCTACCCTGCCTGCAGCCCTGGCAACAC
CTCCACGGCCGAGGAGGAGCTCTGTCGCCTTAAGCTGCTGGCCAAGCACCCCTGCCACATCAAGAAGTTC
GACCGCTACAAGTTTGAGATTACCGTGGGCATGCCATTCAGCAGCGGCGCTGACGGCTCGCGCAGCCGCG
AGGAGGACGACGTCACCAAGGACATCGTGCTGCGGGCCAGCAGCGAGTTCAAGAGCGTGCTGCTCAGCCT
GCGCCAGGGCAGCCTCATCGAGTTCAGCACCATCCTGGAGGGCCGCCTGGGCAGCAAGTGGCCTGTCTTC
GAGCTCAAGGCCATCAGCTGCCTCAACTGCATGGCCCAGCTCTCACCCACCAGGCGGCACGTGAAGATCG
AGCACGACTGGCGCAGCACCGTGCATGGCGCCGTGAAGTTCGCCTTCGACTTCTTTTTCTTCCCATTCCT
GTCGGCGGCCTGA


Restriction Sites SgfI-MluI     
ACCN NM_001145853
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001145853.1, NP_001139325.1
RefSeq Size 3636 bp
RefSeq ORF 2673 bp
Locus ID 7466
Cytogenetics 4p16.1
Protein Families Druggable Genome, Transmembrane
Gene Summary 'This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]'
Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, resulting in a slightly shorter transcript, as compared to variant 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.