TYW1B (NM_001145441) Human Untagged Clone

CAT#: SC326578

TYW1B (untagged)-Human tRNA-yW synthesizing protein 1 homolog B (non-protein coding) (TYW1B), transcript variant 2, mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TYW1B
• Synonyms: LINC00069; NCRNA00069; RSAFD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145441, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCCTTCTGCGGATACATGGGACCTCTCCTCACCTTTAATATCATTATGGATAAACAGGTTTTACA
  TTTATCTGGGCTTTGCTGTTAGCATTAGCCTTTGGATTTGTGTCCAGATTGTCATCGAGATGCAGGGATT
  TGCAACAGTTCTTGCTGAAGCAGTTACGTCCCTGGATCTGCCTGTGGCCATTATTAATCTAAAAGAATAT
  GATCCAGATGATCATCTGATAGAAGAGGAGGAAGAACCCTTCGAGAGCTCCAGTGAAGAAGAGTTTGGTG
  GTGAGGACCATCAGAGCCTAAATTCCATTGTTGATGTTGAAGATTTGGGCAAAATTATGGATCACGTGAA
  GAAAGAAAAGAGAGAAAAGGAACAGCAGGAAGAGAAGTCTGGTTTGTTCAGGAACATGGGGAGGAATGAA
  GATGGTGAAAGAAGAGCTATGATAACTCCTGCTCTCCGAGAAGCCCTTACTAAACAAGTCGATGCTCCGA
  GGGAGAGGAGCTTGTTACAAACACACATTCTATGGAATGAGAGCCATCGCTGCATGGAAACCACCCCGAG
  CTTGGCGTGTGCTAATAAATGTGTCTTCTGTTGGTGGCACCACAACAACCCTGTGGGCACTGAATGGTGG
  TGGAAGATGGACCAGCCTGAAATGATCTTGAAGGAAGCCATTGAAAACCATCAGAACATGATTAAGCAGT
  TTAAAGGAGTACCGGGCGTCAAAGCAGAACGCTTTGAAGAAGGAATGACGGTAAAGCACTGTGCATTGTC
  CCTCGTGGGAGAACCAATAATGTACCCAGAGATCAACAGGTTTTTGAAGCTACTCCACCAGTGTAAAATC
  TCCAGCTTCCTGGTCACAAATGCACAATTTCCTGCGGAAATCAGGAACCTCGAGCCAGTTACTCAGCTGT
  ATGTCAGTGTGGATGCCAGTACCAAAGACAGCCTGAAGAAAATCGACCGCCCACTCTTCAAGGATTTCTG
  GCAGCAATTCCTTGACAGTTTAAAAGCCTTGGCAGTCAAGCAACAACGAACTGTCTACAGACTGACGCTC
  GTGAAAGCATGGAACGTGGACGAGCTCCAGGCCTACGCGCAGCTCGTGTCCCTGGGGAATCCTGACTTCA
  TCGAAGTGAAGGGCGTTACCTACTGCAGAGAAAGTTCAGCAAGCAGTCTTACCATGGCCCATGTGCCCTG
  GCATGAGGAAGTGGTACAGTTTGTCCGCGAGCTGGTGGATCTGATCCCCGAATATGAAATTGCATGTGAA
  CACGAACACTCTAATTGCCTCCTGATAGCACACAGAAAGACAGTTTTAGGAGGGACACCAGGCCATGGAG
  TCACAGAAATCCAGTGGGAAGAGTGGATCAAGAAAAGACTAATCAGCCAGGCACGGTGGCTCATGTCTGT
  TATCCCAGCACATTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145441
• ORF Size: 1416 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001145441.1, NP_001138913.1
• RefSeq Size: 1603
• RefSeq ORF: 1416
• Locus ID: 441250
• Gene Summary: Wybutosine is a hypermodified guanosine found in phenylalanine tRNA. Wybutosine functions to stabilize codon-anticodon interactions during ribosome decoding and therefore supports the maintenance of the reading frame. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. The human genome contains two closely related genes that putatively function in wybutosine synthesis. The open reading frame of this locus is disrupted in some individuals. Thus, this locus appears to be an evolving pseudogene, but may still be functional in some members of the population. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 5' coding region and uses an alternate 3' coding exon, compared to variant 1. The resulting isoform (2) lacks an internal segment near the N-terminus and has a distinct C-terminus, compared to isoform 1.