Nuclear Factor 1 (NFIA) (NM_001145512) Human Untagged Clone

CAT#: SC326590

NFIA (untagged)-Human nuclear factor I/A (NFIA), transcript variant 4, mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFIA
• Synonyms: BRMUTD; CTF; NF-I/A; NF1-A; NFI-A; NFI-L
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001145512 edited
  ATGCAAATGTGCCGCCCGGCCTCCTCCTCGGTTCTCTACGTGCCCACGCGGTGGCCCGGG
  GGGTGCGGGGCAACCTGGCAAAGTTGCCCAAGTCCTCCACCGAGACGCACACGCATACCC
  CAGCGCCCGGCAGTTATGTATTCTCCGCTCTGTCTCACCCAGGATGAATTTCATCCTTTC
  ATCGAAGCACTTCTGCCCCACGTCCGAGCCTTTGCCTACACATGGTTCAACCTGCAGGCC
  CGAAAACGAAAATACTTCAAAAAACATGAAAAGCGTATGTCAAAAGAAGAAGAGAGAGCC
  GTGAAGGATGAATTGCTAAGTGAAAAACCAGAGGTCAAGCAGAAGTGGGCATCTCGACTT
  CTGGCAAAGTTGCGGAAAGATATCCGACCCGAATATCGAGAGGATTTTGTTCTTACAGTT
  ACAGGGAAAAAACCTCCATGTTGTGTTCTTTCCAACCCAGACCAGAAAGGCAAGATGCGA
  AGAATTGACTGCCTCCGCCAGGCAGATAAAGTCTGGAGGTTGGACCTTGTTATGGTGATT
  TTGTTTAAAGGTATTCCGCTGGAAAGTACTGATGGCGAGCGCCTTGTAAAGTCCCCACAA
  TGCTCTAATCCAGGGCTCTGTGTCCAACCCCATCACATAGGGGTTTCTGTTAAGGAACTC
  GATTTATATTTGGCATACTTTGTGCATGCAGCAGATTCAAGTCAATCTGAAAGTCCCAGC
  CAGCCAAGTGACGCTGACATTAAGGACCAGCCAGAAAATGGACATTTGGGCTTCCAGGAC
  AGTTTTGTCACATCAGGTGTTTTTAGTGTCACTGAGCTAGTAAGAGTGTCACAGACACCA
  ATAGCTGCAGGAACTGGCCCAAATTTTTCTCTCTCAGATTTGGAAAGTTCTTCATACTAC
  AGCATGAGTCCAGGAGCAATGAGGAGGTCTTTACCCAGCACATCCTCTACGAGCTCCACA
  AAGCGCCTCAAGTCTGTGGAGGATGAAATGGACAGTCCTGGTGAGGAGCCATTTTATACA
  GGCCAAGGGCGCTCCCCAGGAAGTGGCAGTCAGTCAAGTGGATGGCATGAAGTGGAGCCA
  GGAATGCCATCTCCAACCACACTGAAGAAGTCGGAGAAGTCTGGTTTCAGCAGCCCCTCC
  CCTTCACAGACCTCCTCCCTGGGAACGGCGTTCACACAGCATCACCGACCTGTCATTACA
  GGACCCAGAGCAAGTCCGCATGCAACACCATCGACTCTTCATTTCCCGACATCACCCATT
  ATCCAGCAGCCTGGGCCTTACTTCTCACACCCAGCCATCCGCTATCACCCTCAGGAGACG
  CTGAAAGAATTTGTCCAACTTGTCTGCCCTGATGCTGGTCAGCAGGCTGGACAGGTGGGG
  TTCCTCAATCCCAATGGGAGCAGCCAAGGCAAGGTGCACAACCCATTCCTTCCCACCCCA
  ATGTTGCCACCGCCACCGCCACCACCGATGGCCAGGCCTGTGCCTCTGCCGGTGCCAGAC
  ACAAAGCCTCCAACCACGTCAACAGAAGGAGGTGCAGCCTCCCCCACGTCACCAACCTAC
  TCGACACCCAGCACCTCCCCCGCAAACCGATTCGTCAGTGTTGGACCACGGGATCCAAGC
  TTTGTAAATATCCCTCAACAGACACAGTCCTGGTACCTGGGATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001145512
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145512.1, NP_001138984.1
• RefSeq Size: 9228 bp
• RefSeq ORF: 1665 bp
• Locus ID: 4774
• Cytogenetics: 1p31.3
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (4) differs in the 5' UTR and coding region compared to variant 1. The resulting protein (isoform 4) has a longer and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.