Ki67 (MKI67) (NM_001145966) Human Untagged Clone

CAT#: SC326651

MKI67 (untagged)-Human antigen identified by monoclonal antibody Ki-67 (MKI67), transcript variant 2, mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MKI67
• Synonyms: KIA; MIB-; MIB-1; PPP1R105
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145966, the custom clone sequence may differ by one or more nucleotides
  ATGTGGCCCACGAGACGCCTGGTTACTATCAAAAGGAGCGGGGTCGACGGTCCCCACTTT
  CCCCTGAGCCTCAGCACCTGCTTGTTTGGAAGGGGTATTGAATGTGACATCCGTATCCAG
  CTTCCTGTTGTGTCAAAACAACATTGCAAAATTGAAATCCATGAGCAGGAGGCAATATTA
  CATAATTTCAGTTCCACAAATCCAACACAAGTAAATGGGTCTGTTATTGATGAGCCTGTA
  CGGCTAAAACATGGAGATGTAATAACTATTATTGATCGTTCCTTCAGGTATGAAAATGAA
  AGTCTTCAGAATGGAAGGAAGTCAACTGAATTTCCAAGAAAAATACGTGAACAGGAGCCA
  GCACGTCGTGTCTCAAGATCTAGCTTCTCTTCTGACCCTGATGAGAGTGAGGGAATACCT
  TTGAAAAGAAGGCGTGTGTCCTTTGGTGGGCACCTAAGACCTGAACTATTTGATGAAAAC
  TTGCCTCCTAATACGCCTCTCAAAAGGGGAGAAGCCCCAACCAAAAGAAAGTCTCTGGTA
  ATGCACACTCCACCTGTCCTGAAGAAAATCATCAAGGAACAGCCTCAACCATCAGGAAAA
  CAAGAGTCAGGTTCAGAAATCCATGTGGAAGTGAAGGCACAAAGCTTGGTTATAAGCCCT
  CCAGCTCCTAGTCCTAGGAAAACTCCAGTTGCCAGTGATCAACGCCGTAGGTCCTGCAAA
  ACAGCCCCTGCTTCCAGCAGCAAATCTCAGACAGAGGTTCCTAAGAGAGGAGGGAGAAAG
  AGTGGCAACCTGCCTTCAAAGAGAGTGTCTATCAGCCGAAGTCAACATGATATTTTACAG
  ATGATATGTTCCAAAAGAAGAAGTGGTGCTTCGGAAGCAAATCTGATTGTTGCAAAATCA
  TGGGCAGATGTAGTAAAACTTGGTGCAAAACAAACACAAACTAAAGTCATAAAACATGGT
  CCTCAAAGGTCAATGAACAAAAGGCAAAGAAGACCTGCTACTCCAAAGAAGCCTGTGGGC
  GAAGTTCACAGTCAATTTAGTACAGGCCACGCAAACTCTCCTTGTACCATAATAATAGGG
  AAAGCTCATACTGAAAAAGTACATGTGCCTGCTCGACCCTACAGAGTGCTCAACAACTTC
  ATTTCCAACCAAAAAATGGACTTTAAGGAAGATCTTTCAGGAATAGCTGAAATGTTCAAG
  ACCCCAGTGAAGGAGCAACCGCAGTTGACAAGCACATGTCACATCGCTATTTCAAATTCA
  GAGAATTTGCTTGGAAAACAGTTTCAAGGAACTGATTCAGGAGAAGAACCTCTGCTCCCC
  ACCTCAGAGAGTTTTGGAGGAAATGTGTTCTTCAGTGCACAGAATGCAGCAAAACAGCCA
  TCTGATAAATGCTCTGCAAGCCCTCCCTTAAGACGGCAGTGTATTAGAGAAAATGGAAAC
  GTAGCAAAAACGCCCAGGAACACCTACAAAATGACTTCTCTGGAGACAAAAACTTCAGAT
  ACTGAGACAGAGCCTTCAAAAACAGTATCCACTGCAAACAGGTCAGGAAGGTCTACAGAG
  TTCAGGAATATACAGAAGCTACCTGTGGAAAGTAAGAGTGAAGAAACAAATACAGAAATT
  GTTGAGTGCATCCTAAAAAGAGGTCAGAAGGCAACACTACTACAACAAAGGAGAGAAGGA
  GAGATGAAGGAAATAGAAAGACCTTTTGAGACATATAAGGAAAATATTGAATTAAAAGAA
  AACGATGAAAAGATGAAAGCAATGAAGAGATCAAGAACTTGGGGGCAGAAATGTGCACCA
  ATGTCTGACCTGACAGACCTCAAGAGCTTGCCTGATACAGAACTCATGAAAGACACGGCA
  CGTGGCCAGAATCTCCTCCAAACCCAAGATCATGCCAAGGCACCAAAGAGTGAGAAAGGC
  AAAATCACTAAAATGCCCTGCCAGTCATTACAACCAGAACCAATAAACACCCCAACACAC
  ACAAAACAACAGTTGAAGGCATCCCTGGGGAAAGTAGGTGTGAAAGAAGAGCTCCTAGCA
  GTCGGCAAGTTCACACGGACGTCAGGGGAGACCACGCACACGCACAGAGAGCCAGCAGGA
  GATGGCAAGAGCATCAGAACGTTTAAGGAGTCTCCAAAGCAGATCCTGGACCCAGCAGCC
  CGTGTAACTGGAATGAAGAAGTGGCCAAGAACGCCTAAGGAAGAGGCCCAGTCACTAGAA
  GACCTGGCTGGCTTCAAAGAGCTCTTCCAGACACCAGGTCCCTCTGAGGAATCAATGACT
  GATGAGAAAACTACCAAAATAGCCTGCAAATCTCCACCACCAGAATCAGTGGACACTCCA
  ACAAGCACAAAGCAATGGCCTAAGAGAAGTCTCAGGAAAGCAGATGTAGAGGAAGAATTC
  TTAGCACTCAGGAAACTAACACCATCAGCAGGGAAAGCCATGCTTACGCCCAAACCAGCA
  GGAGGTGATGAGAAAGACATTAAAGCATTTATGGGAACTCCAGTGCAGAAACTGGACCTG
  GCAGGAACTTTACCTGGCAGCAAAAGACAGCTACAGACTCCTAAGGAAAAGGCCCAGGCT
  CTAGAAGACCTGGCTGGCTTTAAAGAGCTCTTCCAGACTCCTGGTCACACCGAGGAATTA
  GTGGCTGCTGGTAAAACCACTAAAATACCCTGCGACTCTCCACAGTCAGACCCAGTGGAC
  ACCCCAACAAGCACAAAGCAACGACCCAAGAGAAGTATCAGGAAAGCAGATGTAGAGGGA
  GAACTCTTAGCGTGCAGGAATCTAATGCCATCAGCAGGCAAAGCCATGCACACGCCTAAA
  CCATCAGTAGGTGAAGAGAAAGACATCATCATATTTGTGGGAACTCCAGTGCAGAAACTG
  GACCTGACAGAGAACTTAACCGGCAGCAAGAGACGGCCACAAACTCCTAAGGAAGAGGCC
  CAGGCTCTGGAAGACCTGACTGGCTTTAAAGAGCTCTTCCAGACCCCTGGTCATACTGAA
  GAAGCAGTGGCTGCTGGCAAAACTACTAAAATGCCCTGCGAATCTTCTCCACCAGAATCA
  GCAGACACCCCAACAAGCACAAGAAGGCAGCCCAAGACACCTTTGGAGAAAAGGGACGTA
  CAGAAGGAGCTCTCAGCCCTGAAGAAGCTCACACAGACATCAGGGGAAACCACACACACA
  GATAAAGTACCAGGAGGTGAGGATAAAAGCATCAACGCGTTTAGGGAAACTGCAAAACAG
  AAACTGGACCCAGCAGCAAGTGTAACTGGTAGCAAGAGGCACCCAAAAACTAAGGAAAAG
  GCCCAACCCCTAGAAGACCTGGCTGGCTTGAAAGAGCTCTTCCAGACACCAGTATGCACT
  GACAAGCCCACGACTCACGAGAAAACTACCAAAATAGCCTGCAGATCACAACCAGACCCA
  GTGGACACACCAACAAGCTCCAAGCCACAGTCCAAGAGAAGTCTCAGGAAAGTGGACGTA
  GAAGAAGAATTCTTCGCACTCAGGAAACGAACACCATCAGCAGGCAAAGCCATGCACACA
  CCCAAACCAGCAGTAAGTGGTGAGAAAAACATCTACGCATTTATGGGAACTCCAGTGCAG
  AAACTGGACCTGACAGAGAACTTAACTGGCAGCAAGAGACGGCTACAAACTCCTAAGGAA
  AAGGCCCAGGCTCTAGAAGACCTGGCTGGCTTTAAAGAGCTCTTCCAGACACGAGGTCAC
  ACTGAGGAATCAATGACTAACGATAAAACTGCCAAAGTAGCCTGCAAATCTTCACAACCA
  GACCCAGACAAAAACCCAGCAAGCTCCAAGCGACGGCTCAAGACATCCCTGGGGAAAGTG
  GGCGTGAAAGAAGAGCTCCTAGCAGTTGGCAAGCTCACACAGACATCAGGAGAGACTACA
  CACACACACACAGAGCCAACAGGAGATGGTAAGAGCATGAAAGCATTTATGGAGTCTCCA
  AAGCAGATCTTAGACTCAGCAGCAAGTCTAACTGGCAGCAAGAGGCAGCTGAGAACTCCT
  AAGGGAAAGTCTGAAGTCCCTGAAGACCTGGCCGGCTTCATCGAGCTCTTCCAGACACCA
  AGTCACACTAAGGAATCAATGACTAACGAAAAAACTACCAAAGTATCCTACAGAGCTTCA
  CAGCCAGACCTAGTGGACACCCCAACAAGCTCCAAGCCACAGCCCAAGAGAAGTCTCAGG
  AAAGCAGACACTGAAGAAGAATTTTTAGCATTTAGGAAACAAACGCCATCAGCAGGCAAA
  GCCATGCACACACCCAAACCAGCAGTAGGTGAAGAGAAAGACATCAACACGTTTTTGGGA
  ACTCCAGTGCAGAAACTGGACCAGCCAGGAAATTTACCTGGCAGCAATAGACGGCTACAA
  ACTCGTAAGGAAAAGGCCCAGGCTCTAGAAGAACTGACTGGCTTCAGAGAGCTTTTCCAG
  ACACCATGCACTGATAACCCCACGACTGATGAGAAAACTACCAAAAAAATACTCTGCAAA
  TCTCCGCAATCAGACCCAGCGGACACCCCAACAAACACAAAGCAACGGCCCAAGAGAAGC
  CTCAAGAAAGCAGACGTAGAGGAAGAATTTTTAGCATTCAGGAAACTAACACCATCAGCA
  GGCAAAGCCATGCACACGCCTAAAGCAGCAGTAGGTGAAGAGAAAGACATCAACACATTT
  GTGGGGACTCCAGTGGAGAAACTGGACCTGCTAGGAAATTTACCTGGCAGCAAGAGACGG
  CCACAAACTCCTAAAGAAAAGGCCAAGGCTCTAGAAGATCTGGCTGGCTTCAAAGAGCTC
  TTCCAGACACCAGGTCACACTGAGGAATCAATGACCGATGACAAAATCACAGAAGTATCC
  TGCAAATCTCCACAACCAGACCCAGTCAAAACCCCAACAAGCTCCAAGCAACGACTCAAG
  ATATCCTTGGGGAAAGTAGGTGTGAAAGAAGAGGTCCTACCAGTCGGCAAGCTCACACAG
  ACGTCAGGGAAGACCACACAGACACACAGAGAGACAGCAGGAGATGGAAAGAGCATCAAA
  GCGTTTAAGGAATCTGCAAAGCAGATGCTGGACCCAGCAAACTATGGAACTGGGATGGAG
  AGGTGGCCAAGAACACCTAAGGAAGAGGCCCAATCACTAGAAGACCTGGCCGGCTTCAAA
  GAGCTCTTCCAGACACCAGACCACACTGAGGAATCAACAACTGATGACAAAACTACCAAA
  ATAGCCTGCAAATCTCCACCACCAGAATCAATGGACACTCCAACAAGCACAAGGAGGCGG
  CCCAAAACACCTTTGGGGAAAAGGGATATAGTGGAAGAGCTCTCAGCCCTGAAGCAGCTC
  ACACAGACCACACACACAGACAAAGTACCAGGAGATGAGGATAAAGGCATCAACGTGTTC
  AGGGAAACTGCAAAACAGAAACTGGACCCAGCAGCAAGTGTAACTGGTAGCAAGAGGCAG
  CCAAGAACTCCTAAGGGAAAAGCCCAACCCCTAGAAGACTTGGCTGGCTTGAAAGAGCTC
  TTCCAGACACCAATATGCACTGACAAGCCCACGACTCATGAGAAAACTACCAAAATAGCC
  TGCAGATCTCCACAACCAGACCCAGTGGGTACCCCAACAATCTTCAAGCCACAGTCCAAG
  AGAAGTCTCAGGAAAGCAGACGTAGAGGAAGAATCCTTAGCACTCAGGAAACGAACACCA
  TCAGTAGGGAAAGCTATGGACACACCCAAACCAGCAGGAGGTGATGAGAAAGACATGAAA
  GCATTTATGGGAACTCCAGTGCAGAAATTGGACCTGCCAGGAAATTTACCTGGCAGCAAA
  AGATGGCCACAAACTCCTAAGGAAAAGGCCCAGGCTCTAGAAGACCTGGCTGGCTTCAAA
  GAGCTCTTCCAGACACCAGGCACTGACAAGCCCACGACTGATGAGAAAACTACCAAAATA
  GCCTGCAAATCTCCACAACCAGACCCAGTGGACACCCCAGCAAGCACAAAGCAACGGCCC
  AAGAGAAACCTCAGGAAAGCAGACGTAGAGGAAGAATTTTTAGCACTCAGGAAACGAACA
  CCATCAGCAGGCAAAGCCATGGACACACCAAAACCAGCAGTAAGTGATGAGAAAAATATC
  AACACATTTGTGGAAACTCCAGTGCAGAAACTGGACCTGCTAGGAAATTTACCTGGCAGC
  AAGAGACAGCCACAGACTCCTAAGGAAAAGGCTGAGGCTCTAGAGGACCTGGTTGGCTTC
  AAAGAACTCTTCCAGACACCAGGTCACACTGAGGAATCAATGACTGATGACAAAATCACA
  GAAGTATCCTGTAAATCTCCACAGCCAGAGTCATTCAAAACCTCAAGAAGCTCCAAGCAA
  AGGCTCAAGATACCCCTGGTGAAAGTGGACATGAAAGAAGAGCCCCTAGCAGTCAGCAAG
  CTCACACGGACATCAGGGGAGACTACGCAAACACACACAGAGCCAACAGGAGATAGTAAG
  AGCATCAAAGCGTTTAAGGAGTCTCCAAAGCAGATCCTGGACCCAGCAGCAAGTGTAACT
  GGTAGCAGGAGGCAGCTGAGAACTCGTAAGGAAAAGGCCCGTGCTCTAGAAGACCTGGTT
  GACTTCAAAGAGCTCTTCTCAGCACCAGGTCACACTGAAGAGTCAATGACTATTGACAAA
  AACACAAAAATTCCCTGCAAATCTCCCCCACCAGAACTAACAGACACTGCCACGAGCACA
  AAGAGATGCCCCAAGACACGTCCCAGGAAAGAAGTAAAAGAGGAGCTCTCAGCAGTTGAG
  AGGCTCACGCAAACATCAGGGCAAAGCACACACACACACAAAGAACCAGCAAGCGGTGAT
  GAGGGCATCAAAGTATTGAAGCAACGTGCAAAGAAGAAACCAAACCCAGTAGAAGAGGAA
  CCCAGCAGGAGAAGGCCAAGAGCACCTAAGGAAAAGGCCCAACCCCTGGAAGACCTGGCC
  GGCTTCACAGAGCTCTCTGAAACATCAGGTCACACTCAGGAATCACTGACTGCTGGCAAA
  GCCACTAAAATACCCTGCGAATCTCCCCCACTAGAAGTGGTAGACACCACAGCAAGCACA
  AAGAGGCATCTCAGGACACGTGTGCAGAAGGTACAAGTAAAAGAAGAGCCTTCAGCAGTC
  AAGTTCACACAAACATCAGGGGAAACCACGGATGCAGACAAAGAACCAGCAGGTGAAGAT
  AAAGGCATCAAAGCATTGAAGGAATCTGCAAAACAGACACCGGCTCCAGCAGCAAGTGTA
  ACTGGCAGCAGGAGACGGCCAAGAGCACCCAGGGAAAGTGCCCAAGCCATAGAAGACCTA
  GCTGGCTTCAAAGACCCAGCAGCAGGTCACACTGAAGAATCAATGACTGATGACAAAACC
  ACTAAAATACCCTGCAAATCATCACCAGAACTAGAAGACACCGCAACAAGCTCAAAGAGA
  CGGCCCAGGACACGTGCCCAGAAAGTAGAAGTGAAGGAGGAGCTGTTAGCAGTTGGCAAG
  CTCACACAAACCTCAGGGGAGACCACGCACACCGACAAAGAGCCGGTAGGTGAGGGCAAA
  GGCACGAAAGCATTTAAGCAACCTGCAAAGCGGAAGCTGGACGCAGAAGATGTAATTGGC
  AGCAGGAGACAGCCAAGAGCACCTAAGGAAAAGGCCCAACCCCTGGAAGATCTGGCCAGC
  TTCCAAGAGCTCTCTCAAACACCAGGCCACACTGAGGAACTGGCAAATGGTGCTGCTGAT
  AGCTTTACAAGCGCTCCAAAGCAAACACCTGACAGTGGAAAACCTCTAAAAATATCCAGA
  AGAGTTCTTCGGGCCCCTAAAGTAGAACCCGTGGGAGACGTGGTAAGCACCAGAGACCCT
  GTAAAATCACAAAGCAAAAGCAACACTTCCCTGCCCCCACTGCCCTTCAAGAGGGGAGGT
  GGCAAAGATGGAAGCGTCACGGGAACCAAGAGGCTGCGCTGCATGCCAGCACCAGAGGAA
  ATTGTGGAGGAGCTGCCAGCCAGCAAGAAGCAGAGGGTTGCTCCCAGGGCAAGAGGCAAA
  TCATCCGAACCCGTGGTCATCATGAAGAGAAGTTTGAGGACTTCTGCAAAAAGAATTGAA
  CCTGCGGAAGAGCTGAACAGCAACGACATGAAAACCAACAAAGAGGAACACAAATTACAA
  GACTCGGTCCCTGAAAATAAGGGAATATCCCTGCGCTCCAGACGCCAAAATAAGACTGAG
  GCAGAACAGCAAATAACTGAGGTCTTTGTATTAGCAGAAAGAATAGAAATAAACAGAAAT
  GAAAAGAAGCCCATGAAGACCTCCCCAGAGATGGACATTCAGAATCCAGATGATGGAGCC
  CGGAAACCCATACCTAGAGACAAAGTCACTGAGAACAAAAGGTGCTTGAGGTCTGCTAGA
  CAGAATGAGAGCTCCCAGCCTAAGGTGGCAGAGGAGAGCGGAGGGCAGAAGAGTGCGAAG
  GTTCTCATGCAGAATCAGAAAGGGAAAGGAGAAGCAGGAAATTCAGACTCCATGTGCCTG
  AGATCAAGAAAGACAAAAAGCCAGCCTGCAGCAAGCACTTTGGAGAGCAAATCTGTGCAG
  AGAGTAACGCGGAGTGTCAAGAGGTGTGCAGAAAATCCAAAGAAGGCTGAGGACAATGTG
  TGTGTCAAGAAAATAAGAACCAGAAGTCATAGGGACAGTGAAGATATT
  
• Restriction Sites: Please inquire
• ACCN: NM_001145966
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145966.1, NP_001139438.1
• RefSeq Size: 11427 bp
• RefSeq ORF: 8691 bp
• Locus ID: 4288
• Cytogenetics: 10q26.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Gene Summary: 'This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.