Aminomethyltransferase (AMT) (NM_001164711) Human Untagged Clone

CAT#: SC326911

AMT (untagged)-Human aminomethyltransferase (AMT) nuclear gene encoding mitochondrial protein transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMT
• Synonyms: GCE; GCST; GCVT; NKH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164711, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGAGGGCTGTAAGTGTGGTGGCCCGTCTGGGCTTTCGCCTGCAGGCATTCCCCCCGGCCTTGTGTC
  GTCCACTTAGTTGCGCACAGACCAAGATACTTGGTAGTGACCGGGTGAAGCTGATGGAGAGTCTAGTGGT
  TGGAGACATTGCAGAGCTAAGACCAAACCAGGGGACACTGTCGCTGTTTACCAACGAGGCTGGAGGCATC
  TTAGATGACTTGATTGTAACCAATACTTCTGAGGGCCACCTGTATGTGGTGTCCAACGCTGGCTGCTGGG
  AGAAAGATTTGGCCCTCATGCAGGACAAGGTCAGGGAGCTTCAGAACCAGGGCAGAGATGTGGGCCTGGA
  GGTGTTGGATAATGCCCTGCTAGCTCTGCAAGGCCCCACTGCAGCCCAGGTACTACAGGCCGGCGTGGCA
  GATGACCTGAGGAAACTGCCCTTCATGACCAGTGCTGTGATGGAGGTGTTTGGCGTGTCTGGCTGCCGCG
  TGACCCGCTGTGGCTACACAGGAGAGGATGGTGTGGAGATCTCGGTGCCGGTAGCGGGGGCAGTTCACCT
  GGCAACAGCTATTCTGAAAAACCCAGAGGTGAAGCTGGCAGGGCTGGCAGCCAGGGACAGCCTGCGCCTG
  GAGGCAGGCCTCTGCCTGTATGGGAATGACATTGATGAACACACTACACCTGTGGAGGGCAGCCTCAGTT
  GGACACTGGGGAAGCGCCGCCGAGCTGCTATGGACTTCCCTGGAGCCAAGGTCATTGTTCCCCAGCTGAA
  GGGCAGGGTGCAGCGGAGGCGTGTGGGGTTGATGTGTGAGGGGGCCCCCATGCGGGCACACAGTCCCATC
  CTGAACATGGAGGGTACCAAGATTGGTACTGTGACTAGTGGCTGCCCCTCCCCCTCTCTGAAGAAGAATG
  TGGCGATGGGTTATGTGCCCTGCGAGTACAGTCGTCCAGGGACAATGCTGCTGGTAGAGGTGCGGCGGAA
  GCAGCAGATGGCTGTAGTCAGCAAGATGCCCTTTGTGCCCACAAACTACTATACCCTCAAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164711
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164711.1, NP_001158183.1
• RefSeq Size: 2047 bp
• RefSeq ORF: 1044 bp
• Locus ID: 275
• Cytogenetics: 3p21.31
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways, Nitrogen metabolism, One carbon pool by folate
• Gene Summary: 'This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (3) lacks an in-frame exon compared to variant 1. The resulting protein (isoform 3) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.