Aminomethyltransferase (AMT) (NM_001164712) Human Untagged Clone

CAT#: SC326943

AMT (untagged)-Human aminomethyltransferase (AMT) nuclear gene encoding mitochondrial protein transcript variant 4


  "NM_001164712" in other vectors (4)

Reconstitution Protocol

USD 660.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol AMT
Synonyms GCE; GCST; GCVT; NKH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001164712, the custom clone sequence may differ by one or more nucleotides


ATGCAGAGGGCTGTAAGTGTGGTGGCCCGTCTGGGCTTTCGCCTGCAGGCATTCCCCCCGGCCTTGTGTC
GTCCACTTAGTTGCGCACAGGAGGTGCTCCGCAGGACACCGCTCTATGACTTCCACCTGGCCCACGGCGG
GAAAATGGTGGCGTTTGCGGGTTGGAGTCTGCCAGTGCAGTACCGGGACAGTCACACTGACTCGCACCTG
CACACACGCCAGCACTGCTCGCTCTTTGACGTGTCTCATATGCTGCAGACCAAGATACTTGGTAGTGACC
GGGTGAAGCTGATGGAGAGTCTAGTGGTTGGAGACATTGCAGAGCTAAGACCAAACCAGGGGACACTGTC
GCTGTTTACCAACGAGGCTGGAGGCATCTTAGATGACTTGATTGTAACCAATACTTCTGAGGGCCACCTG
TATGTGGTGTCCAACGCTGGCTGCTGGGAGAAAGATTTGGCCCTCATGCAGGACAAGGTCAGGGAGCTTC
AGAACCAGGGCAGAGATGTGGGCCTGGAGGTGTTGGATAATGCCCTGCTAGCTCTGCAAGGCCCCACTGC
AGCCCAGGTACTACAGGCCGGCGTGGCAGATGACCTGAGGAAACTGCCCTTCATGACCAGTGCTGTGATG
GAGGTGTTTGGCGTGTCTGGCTGCCGCGTGACCCGCTGTGGCTACACAGGAGAGGATGGTGTGGAGATCT
CGGTGCCGGTAGCGGGGGCAGTTCACCTGGCAACAGCTATTCTGAAAAACCCAGAGGTGAAGCTGGCAGG
GCTGGCAGCCAGGGACAGCCTGCGCCTGGAGGCAGGCCTCTGCCTGTATGGGAATGACATTGATGAACAC
ACTACACCTGTGGAGGGCAGCCTCAGTTGGACACTGGGGAAGCGCCGCCGAGCTGCTATGGACTTCCCTG
GAGCCAAGGTCATTGTTCCCCAGCTGAAGGGCAGGGTGCAGCGGAGGCGTGTGGGGTTGATGTGTGAGGG
GGCCCCCATGCGGGCACACAGTCCCATCCTGAACATGGAGGGTACCAAGATTGGTACTGTGACTAGTGGC
TGCCCCTCCCCCTCTCTGAAGAAGAATGTGGCGATGGGTTATGTGCCCTGCGAGTACAGTCGTCCAGGGA
CAATGCTGCTGGTAGAGCTTCCTTCAGGACCCTGCTTCTGA


Restriction Sites SgfI-MluI     
ACCN NM_001164712
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001164712.1, NP_001158184.1
RefSeq Size 1955 bp
RefSeq ORF 1161 bp
Locus ID 275
Cytogenetics 3p21.31
Protein Pathways Glycine, serine and threonine metabolism, Metabolic pathways, Nitrogen metabolism, One carbon pool by folate
Gene Summary 'This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]'
Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region that results in a frameshift compared to variant 1. The resulting protein (isoform 4) is shorter and has a distinct C-terminus compared to isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.